YAN Yanhua.Effects of Different Exogenous Hormones on Seed Germination and Seedling Growth of Datura stramonium[J].Northern Horticulture,2020,44(23):115-122.[doi:10.11937/bfyy.20200350]
不同外源激素处理对曼陀罗种子萌发及幼苗生长的影响
- Title:
- Effects of Different Exogenous Hormones on Seed Germination and Seedling Growth of Datura stramonium
- Keywords:
- Datura stramonium L.; hormones; seed germination; seedling growth
- 文献标志码:
- A
- 摘要:
- 以栽培和野生曼陀罗种子为试材，采用浸种发芽试验，研究吲哚乙酸(IAA)、赤霉素(GA3)、萘乙酸(NAA)、2,4-二氯苯氧乙酸(2,4-D)4种激素对曼陀罗种子萌发及幼苗生长的影响。结果表明：90 mg?L-1的GA3处理24 h后，栽培种和野生种曼陀罗种子的发芽率和幼苗的鲜质量、干质量、根长以及根表面积均显著提高。NAA处理后，低浓度（10-6~10-8 mol?L-1）时促进2种曼陀罗种子的发芽以及幼苗的生长，高浓度（10-5~10-4 mol?L-1）时抑制2种曼陀罗种子的发芽和幼苗的生长。当2,4-D浓度为1.0 mg?L-1〖JP+2〗时，野生种曼陀罗种子的发芽率和幼苗长势大幅度提高，而栽培种曼陀罗则在浓度为5.0 mg?L-1时发芽率和幼苗长势最佳。IAA处理后栽培种和野生种曼陀罗种子的发芽率和幼苗各项指标均在浓度为25 mg?L-1时达到最大值。综上所述，栽培种曼陀罗种子在NAA浓度为10-8 mol?L-1的处理下发芽率最高，在IAA浓度为25 mg?L-1的处理下幼苗长势最好；野生种曼陀罗种子的发芽率最高以及幼苗长势最好的IAA浓度为25 mg?L-1。该研究为曼陀罗的种植和推广提供了参考依据。
- Abstract:
- The cultivated and wild seeds of Datura stramonium were used as materials to explore the effect of different concentrations of GA3,NAA,2,4-D and IAA on seeds germination and seedling growth by seed soaking.The results showed that after soaking seeds with GA3 at 90 mg?L-1 for 24 hours,the germination rate and fresh weight,dry weight,root length and root surface area of the cultivated and wild seeds were significantly increased.After soaking seeds with NAA,the seed germination and seedling growth of two kinds of seeds were promoted at low concentration(10-6-10-8 mol?L-1),while the germination and seedling growth were inhibited at high concentration(10-5-10-4 mol?L-1).When the concentration of 2,4-D was 1.0 mg?L-1,the germination rate and seedling growth of wild seeds increased significantly,while that of cultivated seeds was the best at 5.0 mg?L-1.After soaking seeds with IAA,the germination rate and seedling growth of the cultivated and wild seeds reached the maximum at 25 mg?L-1.In a word,the germination rate of cultivated seeds was the highest under 10-8 mol?L-1 NAA treatment,while the seedling growth was the best under 25 mg?L-1 IAA treatment,and 25 mg?L-1 IAA was the best treatment for germination rate and seedling growth of wild seeds.This study would provide a theoretical basis for planting and popularizing Datura stramonium L..
参考文献/References:
[1]高春智,何炎红,田有亮,等.不同浓度赤霉素浸种对樟子松种子萌发的影响[J].内蒙古农业大学学报(自然科学版),2012(3):67-72.[2]邹竣竹.激素浸种对野牛草种子发芽与幼苗生长生理的影响[D].北京:中国林业科学研究院,2017.[3]李志,叶青雷,张跃辉,等.不同浓度赤霉素对解除秋雨桑种子休眠的影响[J].种子世界,2015(6):34-35.[4]李欣欣,赵静,廖红.吲哚乙酸、吲哚丁酸和萘乙酸对大豆幼根生长的影响[J].植物生理学报,2013,49(6):572-578.[5]卿纯,张卉灵,林毅青,等.生长素吲哚乙酸对铜绿微囊藻生理生化及产毒特性的影响[J].水生生物学报,2018,42(4):832-838.[6]潘瑞帜,董愚得.植物生理学[M].3版.北京:高等教育出版社,1995.[7]沈同,王镜岩.生物化学[M].2版.北京:高等教育出版社,1990.[8]周淼平,黄益洪,朱作为,等.低浓度2,4-D及外源激素诱导小麦体细胞快速成苗[J].江苏农业学报,2000(3):139-142.[9]中国科学院中国植物志编辑委员会编.中国植物志[M].北京:科学出版社,1980.[10]宋立人.中华本草[M].上海:上海科学技术出版社,1999.[11]邓朝晖,罗充,刘彬,等.曼陀罗药用价值的开发和利用[J].现代生物医学进展,2011,11(7):1394-1398.[12]徐美勤,孙正明,季建林.东莨菪碱和氯丙嗪联合戒毒的疗效分析[J].临床精神医学杂志,2001(5):261-262.[13]ELBADRI G A,LEE D W,PARK J C,et al.Evaluation of various plant extracts for their nematicidal efficacies against juveniles of Meloidogyne incognita[J].Journal of Asia-pacific Entomology,2008,11(2):99-102.[14]邢维贺,阮成江,李贺.5种能源植物种子含油量与脂肪酸组成[J].可再生能源,2010,28(2):62-66.
相似文献/References:
[1]马玲玲,王 鹏,王淑安,等.取材时间和激素对“豫楸1号”腋芽诱导的影响[J].北方园艺,2014,38(13):84.
MA Ling-ling,WANG Peng,WANG Shu-an,et al.Effect of Sampling Time and Hormones on Axillary Buds Induction of ‘Yuqiu No.1’[J].Northern Horticulture,2014,38(23):84.
[2]鲍林文,陈哲皓,钟丹,等.不同激素组合对黄瓜“露地二号”离体子叶再生芽诱导的影响[J].北方园艺,2013,37(13):100.
BAO Lin-wen,CHEN Zhe-hao,ZHONG Dan,et al.Effects of Different Hormones on Buds Regeneration Induction from ?Cotyledons in Cucumber ‘Ludi No.2’[J].Northern Horticulture,2013,37(23):100.
[3]黄俊轩,刘艳军,李建科,等.以花椰菜花球为外植体的离体再生体系的建立[J].北方园艺,2014,38(05):88.
HUANG Jun-xuan,LIU Yan-jun,LI Jian-ke,et al.Regeneration of Cauliflower by in vitro Flower Bulb[J].Northern Horticulture,2014,38(23):88.
[4]韦善君,武运芳,杨林,等.新疆雪莲叶片高效再生植株体系的建立[J].北方园艺,2014,38(05):98.
WEI Shan-jun,WU Yun-fang,YANG Lin,et al.Establishment of High Frequency Plant Regeneration System From the Leaf of Saussurea involucrate[J].Northern Horticulture,2014,38(23):98.
[5]郭朋伟,高晔华,高日,等.“贝吉佳”草莓脱毒苗增殖的研究[J].北方园艺,2013,37(10):110.
GUO Peng-wei,GAO Ye-hua,GAO Ri,et al.Study on Proliferation of Virusfree Strawberry ‘Bechika’[J].Northern Horticulture,2013,37(23):110.
[6]高 婷.桂花嫩枝扦插繁殖技术[J].北方园艺,2013,37(18):78.
GAO Ting.Technology of Softwood Cuttings Propagation in Osmanthus fragrans (Thunb) Lour.[J].Northern Horticulture,2013,37(23):78.
[7]邵元健,周蒋陈.一品红组培苗快繁技术的研究[J].北方园艺,2012,36(02):127.
SHAO Yuan-jian,ZHOU Jiang-chen.Study on the Tissue Culture Technology of the Seedling Propagation in Euphorbia pulcherrima Wild[J].Northern Horticulture,2012,36(23):127.
[8]金亚征,姚太梅,丁丽梅,等.果树花芽分化机理研究进展[J].北方园艺,2013,37(07):193.
JIN Ya-zheng,YAO Tai-mei,DING Li-mei,et al.Research Progress on the Mechanism of Flower Bud Differentiation of Fruit Trees[J].Northern Horticulture,2013,37(23):193.
[9]高晔华,郭朋伟,高 日,等.黑果腺肋花楸组培苗生根培养及驯化的研究[J].北方园艺,2013,37(09):105.
GAO Ye-hua,GUO Peng-wei,GAO Ri,et al.Study on Rooting and Acclimatization in the Process of Tissue Culture of Aronia melanocarpa [J].Northern Horticulture,2013,37(23):105.
[10]高晔华,郭朋伟,吴荣哲.黑果腺肋花楸组培苗增殖的初步研究[J].北方园艺,2012,36(17):119.
GAO Ye-hua,GUO Peng-wei,WU Rong-zhe.Primary Study on Tissue Culture Proliferation of Aronia melanocarpa[J].Northern Horticulture,2012,36(23):119.
备注/Memo
作者简介：闫艳华（1985-），女，硕士，讲师，现主要从事植物生长与发育指标等研究工作。E-mail:yanhua19852008@163.com.基金项目：山西省高等学校科技创新计划资助项目（2020L0692）；山西省重点实验室计划资助项目（201805D111012）；山西省重点研发计划资助项目（201803D221011-6）；吕梁市引进高层次科技人才专业科技创新平台建设资助项目（2018-21-11）。收稿日期：2020-01-31