RAO Junfeng,LYU Weide,SUN Junwei.Biochemical and Physiological Effect of Plant Growth Regulators and Fertilizer on Crocus sativus L.[J].Northern Horticulture,2020,44(18):122-126.[doi:10.11937/bfyy.20194444]
植物生长调节物质和肥料对番红花生理生化的影响
- Title:
- Biochemical and Physiological Effect of Plant Growth Regulators and Fertilizer on Crocus sativus L.
- Keywords:
- saffron (Crocus sativus L.); plant growth regulators; commercial fertilizer; antioxidant system; corm yield
- 文献标志码:
- A
- 摘要:
- 以番红花为试材，采用大田试验的方法，研究了植物生长调节物质（DTA-6、芸薹素内酯）和商品肥（杜高活力钙、杜高活力藻+高力金）喷施后叶片生理生化的变化，以期阐明番红花产量改变的机理。结果表明：喷施植物生长调节剂和商品肥后，叶绿素a(Chl a)、叶绿素b(Chl b)、叶绿素总量(Chl)、类胡萝卜素（Car）含量、可溶性蛋白质含量和可溶性糖含量分别增加了-1.3%~21.8%、21.3%~56.9%、5.2%~22.5%、33.2%~59.5%、0.5%~2.2%、8.2%~21.3%、子球茎产量增加了6.8%~13.9%，而超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性分别提高了24.7%~41.2%、2.6%~22.2%、0.3%~37.5%，膜脂过氧化产物丙二醛（MDA）含量降低了3.9%~32.0%。喷施植物生长调节物质的效果要优于商品肥，而以喷施芸薹素内酯的效果最佳。推测子球茎产量的升高是由于喷施植物生长调节物质和商品肥提高了叶片抗氧化系统清除活性氧的能力，从而使叶绿素含量和光合作用得以提高，延缓叶片的衰老所致。
- Abstract:
- Taking saffron (Crocus sativus L.) as test material,a field experimant was conducted to study the physiological and biochemical changes of its leaves after spraying plant growth regulators (DTA-6,brassinolide) and commercial fertilizers (Dugao-active calcium,Dugao-active algae+Gaolijin) were investigated to clarify the mechanisms of its yield variation.The results showed that after spraying plant growth regulators and commercial fertilizers,compared with control,Chl a content,Chl b content,Car content,soluble protein content and soluble sugar content increased by -1.3%-21.8%,21.3%-56.9%,5.2%-22.5%,33.2%-59.5%,0.5%-2.2%,8.2%-21.3% respectively,and corm yield increased by 6.8%-13.9%.Meanwhile,the activities of antioxidant enzymes SOD,POD and CAT increased by 24.7%-41.2%,2.6%-22.2%,0.3%-37.5% respectively,and the content of MDA,a product of membrane lipid peroxidation,decreased by 3.9%-32.0%.The effect of spraying plant growth regulators was better than commercial fertilizer with the best by spraying brassinolide.It is speculated that the increase of corm yield is due to the improvement of the ability of scavenging reactive oxygen species in antioxidant system (SOD,POD,CAT activities and Car content) of leaves by spraying treatment,thus increasing chlorophyll content and photosynthesis,and delaying the senescence of leaves.
参考文献/References:
[1]《中华本草》编委会.中华本草[M].上海：上海科学技术出版社,1999.[2]ZANIER R,CAIOLA M G.Self incompatibility mechanisms in the Crocus sativus aggregate (Iridaceae)：A preliminary investigation[J].Annali Di Botanica,2011,58(1):83-90.[3]FERNNDEZ J A,PANDALAI S.Biology,biotechnology and biomedicine of saffron[J].Recent Res Dev Plant Sci,2004,2:127-159.[4]饶君凤,吕伟德,王根法.浙江省西红花商品种球繁育与立体栽培技术[J].林业实用科技,2012(7)：23-25.[5]张莹莹,陈亚光,郑东鸽,等.种球大小和叶面肥对西红花开花及其产量的影响[J].农业科技通讯,2017(10):114-119.[6]金伟萍,邵泱峰,徐永清.单季稻接茬西红花高效栽培技术[J].上海蔬菜,2012(1):42-43.[7]沈晓霞,姚金富,王志安,等.施肥与覆草方式对西红花生长及其产量的影响[C].杭州：中药材规范化种植研究研讨会，2004.[8]沈晓霞,姚金富,王志安,等.种植密度和氮肥施用量对西红花球茎产量的影响[J].时珍国医国药,2005,16(1):85-86.[9]朱波,施林妹,徐象华,等.不同遮盖方式对番红花生长发育的影响[J].江苏农业科学,2013,41(8):232-233.[10]陈明明,张红瑞,高致明.栽培措施对西红花生理特性及种球产量的影响[J].浙江农业科学,2016,57(3):386-388.[11]庞阿土,薛小红.提高番红花产量的几项试验[J].中草药,1980(11):510-511.[12]王春健,饶君凤.不同培育基质对西红花种球产量和质量的影响[J].江西农业,2018(20):85-85.[13]元玉碧,张红瑞,邹小双,等.不同基质对西红花生理生化代谢和繁殖系数的影响[J].江苏农业科学,2014,42(5):202-204.[14]薛小红,庞阿土,沈立荣.油菜内酯对番红花产量影响的研究[J].中药材,1991(12):6-7.[15]柯爱萍,薛小红.油菜素内酯对番红花的增产效果[J].中草药,2000,31(9):708-709.[16]施林妹,徐象华,朱波,等.西红花主要活性成分含量变异规律[J].中国实验方剂学杂志,2014,20(13):113-116.[17]张衡锋,韦庆翠,魏欣,等.氮磷钾配施对番红花品质和产量的影响[J].江苏农业科学,2018,46(24):126-129.[18]关力,李楠,王高伟,等.西红花专用肥对西红花球茎产量的影响研究[J].黑龙江医药,2011(4):578-579.[19]郁海金,黄雷.颗粒商品有机肥施肥量对西红花产量的影响[J].江北农业科学,2010,14(9):61-62.[20]王一丹,朱培民,赵伟成.叶面肥对培育西红花大球茎提高花蕊产量的影响[J].上海农业科技,2008(4):101-101.[21]蔡冲.植物生物学实验[M].北京：北京师范大学出版社.2013.[22]许大全,张玉忠,张荣铣.植物光合作用的光抑制[J].植物生理学通讯,1992,28(4):237-243.[23]杜研,杨文忠,孙林琦,等.不同施肥处理对核桃叶片光合作用和叶绿素荧光特性的影响[J].甘肃农业大学学报,2015,50(4):97-102.[24]王惠群,萧浪涛,李合松,等.矮壮素对马铃薯中薯号光合特征和磷素营养的影响[J].植物营养与肥料学报,2007,13(6):1143-1147.[25]赵晶晶,冯乃杰,郑殿,等.植物生长调节剂对马铃薯叶片生理代谢及产量品质的影响[J].干旱地区农业研究,2017,36(6):154-158.[26]孙骏威,翁晓燕,李峤,等.缺钾对水稻不同品种光合和能量耗散的影响[J].植物营养与肥料学报,2007,13(4):577-584.[27]张楚,张永清,路之娟,等.低氮胁迫对不同苦荞品种苗期生长和根系生理特征的影响[J].西北植物学报,2017,37(7):1331-1339.[28]天宏,沈秀瑛,杨德光,等.水分胁迫及复水对玉米地片叶绿素含量和光合作用的影响[J].杂粮作物,2003,23(1):33-35.[29]唐婧文,梁文斌,邹辉,等.土壤水分胁迫对多花黄精光合使用及叶绿素荧光参数的影响[J].中南林业科技大学学报,2019,39(5):110-118.[30]崔波,程邵丽,袁秀云,等.低温胁迫对白及光合作用及叶绿素荧光参数的影响[J].热带作物学报,2019,40(5):891-897.[31]魏永阳,林云弟,巩素霞,等.胁迫对丁香“瓷蓝”光合作用及叶绿素荧光的影响[J].中国农学通报,2016,32(16):93-97.[32]岳寿松,余松烈.小麦旗叶衰老期间光合作用与叶肉细胞超微结构变化[J].沈阳大学学报,1998,29(1):1-5.[33]赵黎明,郑殿峰,冯乃杰,等.植物生长调节剂对大豆叶片光合特性及糖分积累的影响[J].大豆科学,2008,27(3):442-446.[34]孙骏威.水稻根系、光合和抗氧化酶对干旱胁迫的反应[D].杭州：浙江大学，2004.
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备注/Memo
第一作者简介：饶君凤（1969-），女，硕士，教授，现主要从事中药资源应用与品质评价等研究工作。E-mail:356259284@qq.com.基金项目：〖JP4〗杭州市农业科研主动设计资助项目(20172015A06)。收稿日期：2019-11-27