[1]孙叶芳,郑琪,孙骏威,等.枳椇叶片再生体系的优化研究[J].北方园艺,2013,37(03):112-115.
　SUN Ye-fang,ZHENG Qi,SUN Jun-wei,et al.Study on Optimum for Regeneration System of Leaves in Hovenia acerba[J].Northern Horticulture,2013,37(03):112-115.
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枳椇叶片再生体系的优化研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年03期 页码: 112-115 栏目: 出版日期: 2013-03-30
Title:
Study on Optimum for Regeneration System of Leaves in Hovenia acerba
文章编号:
1001-0009(2013)03-0112-04
作者:
孙叶芳1; 郑琪1; 孙骏威2; 陈珍3; 周丹2
1.绍兴市农业科学研究院，浙江 绍兴 312003；
2.中国计量学院 生命科学学院生物系，浙江 杭州 310018；
3.台州学院 生命科学学院，浙江 台州 318000
Author(s):
SUN Ye-fang1; ZHENG Qi1; SUN Jun-wei2; CHEN Zhen3; ZHOU Dan2
1.Shaoxing Academy of Agriculture Sciences，Shaoxing，Zhejiang 312003；
2.Department of Life Sciences，China Jiliang University，Hangzhou，Zhejiang 310018；
3.School of Life Sciences，Taizhou University，Taizhou，Zhejiang 318000
关键词:
枳椇; 植物生长调节剂; 再生苗叶片; 不定芽诱导; 生根诱导
Keywords:
Hovenia acerba; plant growth regulator; leaf disc of regeneration plants; adventitious buds induction; rooting induction
分类号:
S 718.3；Q 813.1
文献标志码:
A
摘要:
以枳椇组培再生苗的叶片为外植体，进行离体培养植株再生研究。结果表明：外植体宜选择叶色浓绿的成熟叶片，诱导愈伤组织和不定芽的最佳培养基为1/2MS+BA 2.0 mg/L+IAA 1.0 mg/L，诱导率可超过85%，增殖率可超过6.0；最佳生根培养基为1/4MS+NAA 0.1 mg/L，添加2%蔗糖，生根率可达95%以上。
Abstract:
A regeneration protocol for Hovenia acerba were set up via organogenesis from callus derived from leaf disc of regeneration Hovenia acerba plants in vitro.The results showed that the best explants were mature leaves with dark green color，and the best placement way of explants was face-up；the most appropriate medium for the induction of callus and adventitious buds was 1/2MS+BA 2.0 mg/L+IAA 1.0 mg/L，with the induction rate more than 85% and proliferation rate more than 6.0；the most appropriate medium for the rooting was 1/4MS+NAA 0.1 mg/L，with addition of 2% sucrose，in which the rooting rate exceeded 95%.

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备注/Memo

第一作者简介：孙叶芳(1978-)，女，硕士，农艺师，研究方向为经济植物的组织培养。E-mail:yefang524@163.com.

责任作者：孙骏威(1978-)，男，硕士，副教授，硕士生导师，研究方向为经济植物的组织培养。E-mail:juville@cjlu.edu.cn.

更新日期/Last Update: 2014-08-23