ZOU Hui,LIN Jiangbo,DAI Yimin,et al.Effects of Endophyte on the Drought Resistance of Dendrobium officinale Under Drought Stress[J].Northern Horticulture,2020,44(06):119-125.[doi:10.11937/bfyy.20192414]
干旱胁迫下内生真菌对铁皮石斛抗旱性的影响
- Title:
- Effects of Endophyte on the Drought Resistance of Dendrobium officinale Under Drought Stress
- Keywords:
- Dendrobium officinale; Epulorhiza sp.; drought stress
- 文献标志码:
- A
- 摘要:
- 以连城冠豸山的铁皮石斛(Dendrobium officinale)为试材，把内生真菌（瘤菌根菌属）菌液浇灌到铁皮石斛盆栽苗中，并共同培养6个月后进行干旱胁迫，研究了干旱胁迫后铁皮石斛的表型、存活率以及生理生化指标的变化，以期为揭示干旱胁迫下内生真菌（瘤菌根菌属）与铁皮石斛抗旱的关系提供参考依据。结果表明：接菌共培养后的石斛苗长势较好，干旱胁迫后存活力高，与未接菌的对照呈显著差异；接菌后的石斛叶片相对含水量（RWC）、游离脯氨酸（Pro）含量及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性都显著高于对照，而质膜相对透性（PMP）和丙二醛（MDA）含量显著下降，这表明了接菌能够降低膜脂过氧化的伤害，提高了铁皮石斛的存活率和抗旱能力。
- Abstract:
- Dendrobium officinale seedlings from Liancheng Guanzhi Mountain were used as raw material.The fungi (Epulorhiza sp.) were irrigated into the plug seedlings of D.officinale and co-cultivated for 6 months，and subjected to drought stress.The changes of phenotype，survival rate，and physiological and biochemical indexes were assayed respectedly，which would provide a theoretical basis for revealing the relationship between endophytic fungi and D. officinale under drought stress.The results showed that the growth of seedlings has been enhanced by inoculating and co-cultivating with Epulorhiza sp.，and survival rate has been improved highly under drought stress，which was significantly different from the control without inoculation.Relative water content (RWC)，free proline content and the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly higher than those of the control，while the relative permeability of the plasma membrane (PMP) and malondialdehyde (MDA) were decreased significantly，indicating that the Epulorhiza sp.could reduce the damage of membrane lipid peroxidation，improve the survival rate and drought resistance.These results would provide a reference for the drought resistance mechanism，cultivation，production and application of D.officinale.
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备注/Memo
第一作者简介：邹晖(1982-),男，本科，研究实习员，现主要从事植物组织培养等研究工作。E-mail:21880338@qq.com.责任作者：王伟英(1980-)，女，博士，助理研究员，现主要从事植物逆境生理和分子生物学等研究工作。E-mail：weiyingwang178@126.com.基金项目：福建省农业科学院科技创新资助项目（PC2018-13）；福建省属公益类科研院所基本科研专项资助项目（2016R1012-5）。收稿日期：2019-09-26