ZHANG Xuesong,LIU Huanying,XIE Chunqin,et al.Inhibition of Glycosidase by Water Extracts of Rare Edible-medicinal Fungi[J].Northern Horticulture,2020,44(06):126-133.[doi:10.11937/bfyy.20192291]
珍稀食药用菌水提取物对糖苷酶的抑制
- Title:
- Inhibition of Glycosidase by Water Extracts of Rare Edible-medicinal Fungi
- 文献标志码:
- A
- 摘要:
- 以5种食药用菌水提取物为试材，采用测定糖苷酶抑制率的方法，研究了5种珍稀食药用菌水提取物对糖苷酶的抑制作用，以期为进一步开发降血糖药物提供参考依据。结果表明：羊肚菌、桑黄、灵芝、蝉花、虫草水提取物对4种糖苷酶均有不同程度的抑制，且差异显著（P<0.05）。5种水提物中，桑黄水提物对蔗糖酶的抑制率最高（74.8%），略低于常用的降糖药阿卡波糖以及米格列醇，虫草水提物对蔗糖酶的抑制率最低（35.9%）。虫草水提物对α-淀粉酶的抑制能力最高（9.7%）且显著高于米格列醇（P<0.05），羊肚菌水提物对α-淀粉酶的抑制最低（1.1%）。羊肚菌水提物对α-葡萄糖苷酶显示出较好的抑制能力（15.4%），显著高于阿卡波糖和米格列醇（P<0.05），而灵芝水提物对α-葡萄糖苷酶的抑制水平最低（11.2%）。灵芝水提物对麦芽糖酶显示出较高的抑制能力（10.2%），显著高于米格列醇（P<0.05），而与阿卡波糖相当，桑黄水提物对麦芽糖酶抑制最差(4.6%）。5种水提物在20~60 ℃以及pH 6~8 时对4种糖苷酶仍然保持较好的抑制能力。
- Abstract:
- Five kinds of water extracts of rare edible-medicinal fungi were used as material,and inhibition rates of glucosidase were detected to compare their inhibitory effects,in order to provide the basis for development of hypoglycemic drugs.The results showed that the water extracts of morchella,phellinus igniarius,ganoderma lucidum,cordyceps cicadae,and cordyceps militaris had different degrees of inhibition on the four glycosidase,and the difference was significant (P<0.05).Among the five water extracts,the extract of phellinus igniarius had the highest inhibition rate (74.8%),slightly lower than the commonly used hypoglycemic drugs acarbose and miglitol,and the extract of cordyceps militaris of the inhibition rate was the lowest (35.9%).The water extract of cordyceps militaris had the highest inhibition ability of α-amylase (9.7%) and was significantly higher than that of miglitol (P<0.05).The water extract of morchella had the lowest inhibition of α-amylase (1.1%).The water extract of morchella showed good inhibition ability to α-glucosidase (15.4%),which was significantly higher than that of acarbose and miglitol (P<0.05),while the extract of ganoderma lucidum had the lowest level of α-glucosidase inhibition (11.2%).Ganoderma lucidum water extract showed higher inhibition ability (10.2%) of maltolase,which was significantly higher than that of miglitol (P<0.05),but same to acarbose.The water extract of Phellinus chinensis had the lowest inhibition rate of maltase (4.6%).The five aqueous extracts still maintained good inhibition of the four glycosidase enzymes at 20-60 ℃ and pH 6-8.
参考文献/References:
[1]王谦,贾震.食药用真菌的药理作用研究进展[J].医学研究与教育,2010,27(5):67-70.[2]康洁,陈若芸.近10年药用真菌成分及活性研究进展[J].中国药学杂志,2005(2):9-12.[3]MANISH K P,BHAKTI T,AVINASH M,et al.Physicochemical characterization,antioxidant and anti-proliferative activities of a polysaccharide extracted from psyllium (P.ovata) leaves[J].International Journal of Biological Macromolecules,2018,118：976-987.[4]贾少杰,陈文强,解修超,等.秦巴山区猪苓多糖的提取及其脂质体生产工艺的优化[J].安徽农业大学学报,2018,45(4):715-722.[5]XIAO C,WU Q P,ZHANG J M,et al.Antidiabetic activity of Ganoderma lucidum polysaccharides F31 down-regulated hepatic glucose regulatory enzymes in diabetic mice[J].Journal of Ethnopharmacology,2017,196：47-57.[6]张晨.杏鲍菇菌丝体多糖的分离纯化及抗衰老、抗糖尿病活性分析[D].泰安:山东农业大学,2018.[7]RENATA N,NATALIA NJ,MAREK J,et al.The preliminary study of prebiotic potential of Polish wild mushroom polysaccharides:The stimulation effect on Lactobacillus strains growth[J].European Journal of Nutrition,2018,57(4)：1511-1521.[8]陈蒙蒙.三种野生食药用真菌的驯化和培养特性研究[D].烟台:鲁东大学,2017.[9]梁雪,倪秀珍,汉丽萍.食药用真菌多糖生物活性的研究进展[J].长春师范大学学报,2018,37(10):82-85.[10]ZHANG M,CUI S W,CHEUNG P C K,et al.Antitumor polysaccharides from mushrooms:review on their isolation process,structural characteristics and antitumor activity[J].Trends in FoodScience & Technology,2006,18(1):4-19.[11]XIAO C,WU Q P,CAI W,et al.Hypoglycemic effects of Ganoderma lucidum polysaccharides in type 2 diabetic mice[J].Archives of Pharmacal Research,2012,35(10):1793-1801.[12]杨健华.草菇子实体粗多糖免疫功能调节、抗氧化及抗炎作用研究[D].长春:吉林农业大学,2017.[13]尚红梅.小刺猴头菌发酵浸膏多糖的分析及降脂活性研究[D].长春:吉林农业大学,2015.[14]李英,张兰,杨万山,等.α-葡萄糖苷酶抑制剂的筛选和初步研究[J].上海大学学报(自然科学版),2000(2):129-132.[15]朱运平,李秀婷,李里特.天然α-葡萄糖苷酶抑制剂来源及应用研究现状[J].中国食品学报,2011,11(4):154-160.[16]黄聪亮,郑佳俐,李凤林,等.茯苓多糖对Ⅱ型糖尿病小鼠降糖作用研究[J].食品研究与开发,2016,37(4):21-25.[17]尹红力.硫酸酯化黑木耳酸性多糠Cr（Ⅲ）螯合物制备及降血糖功能研究[D].哈尔滨:东北林业大学,2015.[18]RAFAEL M F,VICTOR M R G,RUBEN F G,et al.Effect of different drying procedures on the bioactive polysaccharide acemannan from Aloe vera (Aloe barbadensis Miller)[J].Carbohydrate Polymers,2017，168:327-336.[19]张芳,郭盛,钱大玮,等.枸杞多糖的提取纯化与分子结构研究进展及产业化开发现状与前景分析[J].中草药,2017,48(3):424-432.[20]潘文慧,刘晓东,邓赟,等.麻竹竹叶多糖分离纯化及相对分子质量测定[J].食品科技,2016,41(7):189-191.[21]运立媛,张民,朱振元.不同产地黄芪多糖降血糖活性的比较研究[J].食品研究与开发,2018,39(19):20-25.[22]LIU M,MAO X Q,WU Y,et al.Astragalus polysaccharide improves insulin sensitivity in KKAy mice:Regulation of PKB/GLUT4 signaling in skeletal muscle[J].Journal of Ethnopharmacology,2010,127(1):32-37.[23]刘奕辰.水提醇沉提取银杏叶多糖工艺浸提温度的试验研究[J].山东畜牧兽医,2019,40(2):10-11.[24]陈金龙.化学修饰羊肚菌多糖生物活性研究[D].重庆:西南大学,2017.[25]吴彬彬.具有抑制麦芽糖酶活性的乳酸菌筛选及其特性研究[D].无锡:江南大学,2009.[26]让一峰.白芸豆α-淀粉酶抑制剂的制备及其在低GI方便粥中的应用[D].无锡:江南大学,2016.[27]王璐瑶,吕晓玲,李静,等.紫甘薯花青素在模拟消化道条件下对蔗糖酶酶活性的抑制作用[J].中国食品添加剂,2017(12):119-123.[28]陈海君,马尚智,江敏,等.超滤亲和-液质联用和分子对接技术筛选毛菊苣根中α-葡萄糖苷酶抑制剂[J].中草药,2019,50(2):344-351.[29]WANG Y F,PENG Y H,WEI X L,et al.Sulfation of tea polysaccharides:Synthesis,characterization and hypoglycemic activity[J].International Journal of Biological Macromole Cules,2009,46(2)：270-274.[30]LI S Q,SHAH N P.Antioxidant and antibacterial activities of sulphated polysaccharides from Pleurotus eryngii and Streptococcus thermophilus ASCC 1275[J].Food hemistry,2014,165：262-270.
相似文献/References:
[1]陈世通,白建波,李梦杰,等.食药用菌多糖的药理作用及开发利用研究[J].北方园艺,2012,36(17):195.
CHEN Shi-tong,BAI Jian-bo,LI Meng-jie,et al.Study on Exploitation and Pharmacological Effect on Edible and Pharmaceutical Fungus Polysaccharides[J].Northern Horticulture,2012,36(06):195.
[2]汤昕明,冯云利,郭相,等.我国食药用菌栽培现状分析及展望[J].北方园艺,2019,43(08):148.[doi:10.11937/bfyy.20182324]
TANG Xinming,FENG Yunli,GUO Xiang,et al.Current Situation and Development Trend of Edible Medicinal Fungi in China[J].Northern Horticulture,2019,43(06):148.[doi:10.11937/bfyy.20182324]
备注/Memo
第一作者简介：张雪松（1979-），男，博士，副教授，研究方向为天然产物开发与应用。E-mail:shelz@sina.com.责任作者：谢春芹（1976-），女，硕士，副教授，研究方向为食用菌。E-mail:1106667996@qq.com.基金项目：江苏农林职业技术学院产业创新团队资助项目 (2018kj04)；句容市农业委员会农业课题资助项目（JRNW[2018]06）。收稿日期：2019-09-09