SUN Xiaohua,WANG Xiaobo,DU Xian,et al.Response on Growth,Photosynthetic Performance in Salt-sensitive Melon Seedling Under Salinity Stress[J].Northern Horticulture,2019,43(24):43-50.[doi:10.11937/bfyy.20191604]
盐敏感甜瓜幼苗生长及生理特性对盐胁迫的响应
- Title:
- Response on Growth,Photosynthetic Performance in Salt-sensitive Melon Seedling Under Salinity Stress
- Keywords:
- salinity stress; photosynthesis; chlorophyll fluorescence; antioxidant
- 文献标志码:
- A
- 摘要:
- 以盐敏感甜瓜品种‘羊角酥’为试验材料,利用不同浓度NaCl(12.5、25.0、37.5、50.0、62.5、75.0 mmol?L-1)进行梯度胁迫处理,探讨盐胁迫对甜瓜生长发育、光合作用、叶绿素荧光、脂质过氧化和抗氧化酶活性的影响,旨在为甜瓜盐胁迫的适应性机制提供参考依据。结果表明:不同浓度NaCl(12.5~75.0 mmol?L-1)处理显著降低株高、叶面积,对茎粗无显著影响;与对照相比,盐胁迫下甜瓜幼苗气孔导度(Gs)和蒸腾速率(Tr)显著降低;随着NaCl浓度增加,丙二醛(MDA)含量显著增加;在62.5、75.0 mmol?L-1浓度下,叶绿素(Chl)含量和电子传递速率(ETR)显著降低,而其它叶绿素荧光参数,如开放PSⅡ中心的激发捕获效率(Fv′/Fm′)、光化学淬灭系数(qP)和非光化学淬灭系数(qN)则略有降低,但差异不显著;盐胁迫下甜瓜叶片中超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的变化趋势与盐浓度无相关性。盐敏感甜瓜品种受低盐浓度(<75.0mmol?L-1)的影响主要表现为幼苗生长势和光合效率的降低。此外,盐浓度过高时(75.0 mmol?L-1),由盐胁迫引起的脂质过氧化很难被酶抗氧化剂完全抵消。
- Abstract:
- The objective of this study was to investigate the effect of salinity stress on the growth,photosynthesis,chlorophyll fluorescence,lipid peroxidation and antioxidative enzyme activity of salt-sensitive melon (Cucumis melo L.) cultivar,‘Horn Crisp’ melon.The results showed that different NaCl concentrations (from 12.5 mmol?L-1 to 75.0 mmol?L-1) significantly reduced the growth attributes including plant height and leaf area,but no significant change on stalk diameter.Salt-stressed melon seedling demonstrated a significant decrease in stomatal gsuctance (Gs) and transpiration rate (Tr) compared to control.Meanwhile,a significant increase in malondialdehye (MDA) was exhibited with increasing of NaCl concentration.Under 62.5 mmol?L-1 and 75.0 mmol?L-1 NaCl,chlorophyll (Chl) content and electron transport rate (ETR) had significantly reduced,while other chlorophyll fluorescence parameters,such as efficiency of excitation capture of open PSⅡ centers (Fv′/Fm′),photochemical quenching (qP) and non-photochemical quenching (qN) emerged a slight reduction but no significance.It was noteworthy that the change trends of antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD) activities in melon seedling under salt stress revealed no correlation with salt concentration.These results demonstrated that salt-sensitive melon variety was affected by a low saline concentration range (<75.0 mmol?L-1),melon seedling respond to salinity stress rely on reducing growth potential and photosynthetic efficiency.Moreover,lipid peroxidation induced by salt stress was difficult to offset completely by enzymatic antioxidants when NaCl concentration exceeded a certain range.
参考文献/References:
[1]MACHADO R M A,SERRALHEIRO R P.Soil salinity:Effect on vegetable crop growth.Management practices to prevent and mitigate soil salinization[J].Horticulturae,2017(3):1-13.[2]KAWANAB S,ZHU T.Degeneration and conservational trial of Aneurolepidium chinense grassland in Northern China[J].Journal of the Japanese Society for Grassland Science,1991,39:91-99.[3]WANG H,LIN X,CAO S,et al.Alkali tolerance in rice(Oryza sativa L.):Growth,photosynthesis,nitrogen metabolism,and ion homeostasis[J].Photosynthetica,2015,53:55-65.[4]SLAMA I,ABDELLY C,BOUCHEREAU A,et al.Diversity,distribution and roles of osmoprotective compounds accumulated in halophytes under abiotic stress[J].Annals of Botany,2015,115:433-447.[5]MUNNS R,TESTER M.Mechanisms of salinity tolerance[J].Annu Review of Plant Biology,2008,59:651-681.[6]LI J,LIU J,WANG G,et al.A chaperone function of NO CATALASE ACTIVITY1 is required to maintain catalase activity and for multiple stress responses in Arabidopsis[J].Plant Cell,2015,27:908-925.[7]GENISEL M,ERDAL S,KIZILKAYA M.The mitigating effect of cysteine on growth inhibition in salt-stressed barley seeds is related to its own reducing capacity rather than its effects on antioxidant system[J].Plant Growth Regulation,2015,75:187-197.[8]AMOR N B,MEGDICHE W,JIMENEZ A,et al.The effect of calcium on the antioxidant systems in the halophyte Cakile maritima under salt stress[J].Acta Physiologiae Plantarum,2009,32:453-461.[9]HERNANDEZ J A,FERRER M A,JIMENEZ A,et al.Antioxidant systems and O?[TX-*4]2/H2O2 production in the apoplast of pea leaves.Its relation with salt-induced necrotic lesions in minor veins[J].Plant Physiology,2001,127:817-831.[10]WITZEL K,MATROS A,STRICKERT M,et al.Salinity stress in roots of contrasting barley genotypes reveals time-distinct and genotype-specific patterns for defined proteins[J].Molecular Plant,2014,7:336-355.[11]YANG J C,ZHANG J H,WANG Z Q,et al.Involvement of abscisic acid and cytokinins in the senescence and remobilization of carbon reserves in wheat subjected to water stress during grain filling[J].Plant Cell and Environment,2003,26:1621-1631.[12]KARIMI G,GHORBANLI M,HEIDARI H,et al.The effects of NaCl on growth,water relations,osmolytes and ion content in Kochia prostrata[J].Biologia Plantarum,2005,49:301-304.[13]DASNEVES J P C,FERREIRA L F P,VAZ M M,et al.Gas exchange in the salt marsh species Atriplex portulacoides L.and Limoniastrum monopetalum L.in southern Portugal[J].Acta Physiologiae Plantarum,2008,30:91-97.[14]ZHANG Y Q,KAISER E,ZHANG Y T,et al.Short-term salt stress strongly affects dynamic photosynthesis,but not steady-state photosynthesis,in tomato(Solanum lycopersicum)[J].Environmental and Experimental Botany,2018,149:109-119.[15]RHODES D,NADOLSKA-ORCZYK A,RICH P J.Salinity,osmolytes and compatible solutes[M].Netherlands:Kluwer Academic Publishers,2002:181-204.[16]MUCHATE N S,NIKALJE G C,RAJURKAR N S,et al.Plant salt stress:Adaptive responses,tolerance mechanism and bioengineering for salt tolerance[J].Botanical Review,2016,82:371-406.[17]GARCIA-MAS J,BENJAK A,SANSEVERINO W,et al.The genome of melon (Cucumis melo L.)[J].PNAS,2012,109:11872-11877.[18]WEI S W,GAO L W,ZHANG Y D,et al.Genome-wide investigation of the NAC transcription factor family in melon (Cucumis melo L.) and their expression analysis under salt stress[J].Plant Cell Reports,2016,35:1827-1839.[19]DEINLEIN U,STEPHAN A B,HORIE T,et al.Plant salt-tolerance mechanisms[J].Trends in Plant Science,2014,19:371-379.[20]STAVRIDOU E,HASTINGS A,WEBSTER R J,et al.The impact of soil salinity on the yield,composition and physiology of the bioenergy grass Miscanthus × giganteus[J].Global Change Biology Bioenergy,2017(9):92-104.[21]ACOSTA-MOTOS J R,LVAREZ S,HERNNDEZ J A,et al.Irrigation of Myrtus communis plants with reclaimed water:Morphological and physiological responses to different levels of salinity[J].The Journal of Horticultural Science & Biotechnology,2014,89:487-494.[22]DELAMOR F M,RUIZ-SANCHEZ M C,MARTINEZ V,et al.Gas exchange,water relations,and ion concentrations of salt-stressed tomato and melon plants[J].Journal of Plant Nutrition,2000,23:1315-1325.[23]OLIVEIRA F A,OLIVEIRA M K T,LIMA L A,et al.Desenvolvimento inicial do maxixeiro irrigado com águas de diferentes salinidades[J].Agropecuária Científica,2012(8):22-28.[24]HU K L,ZHANG L,WANG J T,et al.Influence of selenium on growth,lipid peroxidation and antioxidative enzyme activity in melon (Cucumis melo L.) seedlings under salt stress[J].Acta Societatis Botanicorum Poloniae,2013,82:193-197.[25]YAO X,CHU J,WANG G.Effects of selenium on wheat seedlings under drought stress[J].Biological Trace Element Research,2009,130:283-290.[26]ASHRAF M,HARRIS P J C.Photosynthesis under stressful environments:An overview[J].Photosynthetica,2013,51:163-190.[27]MANAA A,AHMED H B,VALOT B,et al.Salt and genotype impact on plant physiology and root proteome variations in tomato[J].Journal of Experimental Botany,2011,62:2797-2813.[28]AKLADIOUS S A,MOHAMED H I.Ameliorative effects of calcium nitrate and humic acid on the growth,yield component and biochemical attribute of pepper (Capsicum annuum) plants grown under salt stress[J].Scientia Horticulturae,2018,236:244-250.[29]BOLUS S T,EL-SHOURBARY M N,MISSAK N L.Studies on the effect of salinity on the epidermis and mesophyll tissues of some Ricinus communis L.varieties leaves[J].Desert Research Institute,1972,22:421-452.[30]LUO J,HUANG C H,PENG F,et al.Effect of salt stress on photosynthesis and related physiological characteristics of Lycium ruthenicum Murr[J].Acta Agriculturae Scandinavica Section B-Soil and Plant Science,2017,67:1-13.[31]SETTER T L,BRUN W A,BRENNER M L.Stomatal closure and photosynthetic inhibition in soybean leaves induced by petiole girdling and pod removal[J].Plant Physiology,1980,65:884-887.[32]CARMO-SILVA A E,SALVUCCI M E.The regulatory properties of Rubisco activase differ among species and affect photosynthetic induction during light transitions[J].Plant Physiology,2013,161:1645-1655.[33]KAISER E,KROMDIJK J,HARBINSON J,et al.Photosynthetic induction and its diffusional,carboxylation and electron transport processes as affected by CO2 partial pressure,temperature,air humidity and blue irradiance[J].Annals of Botany,2017,119:191-205.[34]KALAJI H M,SCHANSKER G,BRESTIC M,et al.Frequently asked questions about chlorophyll fluorescence,the sequel[J].Photosynthesis Research,2017,132:13-66.[35]PARIDA A K,DAS A B.Salt tolerance and salinity effects on plants:A review[J].Ecotoxicology and Environmental Safety,2005,60:324-349.[36]KIANI-POUYA A,RASOULI F.The potential of leaf chlorophyll content to screen bread-wheat genotypes in saline Gsition[J].Photosynthetica,2014,52:288-300.[37]AZIZOV I V,KHANISHEVA M A.Pigment content and activity of chloroplasts of wheat genotypes grown under saline environment[J].P Azerbaijan National Academy Science Biology Science,2010,65:96-98.[38]CUIN T A,PARSONS D,SHABALA S.Wheat cultivars can be screened for NaCl salinity tolerance by measuring leaf chlorophyll content and shoot sap potassium[J].Functional Plant Biology,2010,37:656-664.[39]GOMES M A C,SUZUKI M S,CUNHA M D,et al.Effect of salt stress on nutrient concentration,photosynthetic pigments,proline and foliar morphology of Salvinia auriculata Aubl[J].Acta Limnologica Brasilliensia,2011,23:164-176.[40]CECCARELLI S,GRANDO S,MAATOUGUI M,et al.Plant breeding and climate changes[J].Journal of Agricultural Science,2010,148:627-637.[41]EYIDOGAN F,OZ M T.Effect of salinity on antioxidant responses of chickpea seedlings[J].Acta Physiology Plant,2007,29:485-493.[42]WU F B,ZHANG G P,DOMINY P.Four barley genotypes respond differently to cadmium:Lipid peroxidation and activities of antioxidant capacity[J].Environmental and Experimental Botany,2003,50:67-78.[43]LI J J,MA J J,GUO H L,et al.Growth and physiological responses of two phenotypically distinct accessions of centipedegrass[Eremochloa ophiuroides (Munro) Hack.]to salt stress[J].Plant Physiology and Biochemistry,2018,126:1-10.
相似文献/References:
[1]单守明,平吉成,刘晶,等.6-BA对染卷叶病毒葡萄光合作用和果实品质的影响[J].北方园艺,2013,37(04):5.
[2]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[3]石秋梅,邓翻云,吴敏言,等.罗布麻和大叶白麻种子萌发及幼苗生长耐盐性研究[J].北方园艺,2014,38(12):128.
SHI Qiu-mei,DENG Fan-yun,WU Min-yan,et al. Study on Salt Tolerance of Apocynum venetum Linn.and Poacynum hendersonii(Hook.f.) Woodson at Stages of Seed Germination and Seedlings Growth[J].Northern Horticulture,2014,38(24):128.
[4]张 芬,张 波,田丽萍,等.盐胁迫对番茄幼苗叶片光合特性及叶绿素和β-胡萝卜素含量的影响[J].北方园艺,2014,38(11):15.
ZHANG Fen,ZHANG Bo,TIAN Li-ping,et al.Effect of Salt Stress on Photosynthetic Characteristic and the Content of Chlorophyll and β-carotene in Tomato Seedling Leaves[J].Northern Horticulture,2014,38(24):15.
[5]常青山,张利霞,郑轶琦,等.盐胁迫下钙离子对苜蓿种子萌发的影响[J].北方园艺,2014,38(10):64.
CHANG Qing-shan,ZHANG Li-xia,ZHENG Yi-qi,et al.Effects of Ca2+?on Seed Germination Characteristics of Medicago sativa Under Salt Stress[J].Northern Horticulture,2014,38(24):64.
[6]杨 凯,孙迎坤,谭 雯,等.盐胁迫对香石竹种子萌发及幼苗生长的影响[J].北方园艺,2013,37(22):86.
YANG Kai,SUN Ying-kun,TAN Wen,et al.Effects of Salt Stress on Seeds Germination and Seedling Growth of Carnation[J].Northern Horticulture,2013,37(24):86.
[7]张 娇,张大治,马 艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014,38(14):65.
ZHANG Jiao,ZHANG Da-zhi,MA Yan,et al.Effect of NaCl Simulation of Salt Stress on Seed Germination and Seedling Growth of Ammopiptanthus mongolicus[J].Northern Horticulture,2014,38(24):65.
[8]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(24):1.
[9]童丽丽,王哲宇,吴世光,等.模拟淹水对黄山栾树幼苗的光合特性及部分抗氧化保护酶活性的影响[J].北方园艺,2014,38(01):68.
TONG Li-li,WANG Zhe-yu,WU Shi-guang,et al.Effects of Simulated Waterlogging on Photosynthetic Characteristics and Some Antioxidant Enzymes Activity of Koelreuteria integrifolia[J].Northern Horticulture,2014,38(24):68.
[10]韩春梅,李春龙,叶少平,等.生姜水浸液对生姜幼苗形态和光合指标的影响 [J].北方园艺,2013,37(17):1.
HAN Chun-mei,LI Chun-long,YE Shao-ping,et al.Effects of Ginger Aqueous Extracts on Morphology and Photosynthesis Indexes of Ginger Seedling[J].Northern Horticulture,2013,37(24):1.
[11]鲁少尉,齐飞,李天来.NaCl胁迫对番茄叶片光合特性及蔗糖代谢的影响[J].北方园艺,2012,36(09):14.
LU Shao-wei,QI Fei,LI Tian-lai.Effect of NaCl Stress on Photosynthetic Characteristics and Sucrose Metabolizing in Tomato Leaf[J].Northern Horticulture,2012,36(24):14.
[12]马帅,冯金朝,李熙盟,等.土壤盐胁迫对葡萄光合作用特性的影响[J].北方园艺,2012,36(12):4.
MAShuai,FENGJin-chao,LIXi-meng,et al.ThePhotosynthesisCharacteristicsofGrapeUndertheStressofSoilSalinity[J].Northern Horticulture,2012,36(24):4.
[13]艾楷棋,苏华,周海将,等.不同红光与远红光对盐胁迫下番茄叶绿素合成的影响[J].北方园艺,2019,43(01):14.[doi:10.11937/bfyy.20181267]
AI Kaiqi,SU Hua,ZHOU Haijiang,et al.Effects of Different R∶FR Ratio on Chlorophyll Biosynthesis in Tomato Leaves Under Salt Stress[J].Northern Horticulture,2019,43(24):14.[doi:10.11937/bfyy.20181267]
备注/Memo
第一作者简介:孙晓华(1981-),女,博士,讲师,研究方向为设施园艺作物栽培。E-mail:sungod0819@163.com.责任作者:李晓静(1974-),女,博士,副教授,研究方向为设施作物栽培生理。E-mail:lxjdcg@163.com.基金项目:内蒙古自治区高等学校科学技术研究资助项目(NJZY20047)。收稿日期:2019-07-15