LIU Junjuan.Effects of High Temperature and Drought Stress on PSII Function and Light Distribution in Peony Leaves With Different Resistance[J].Northern Horticulture,2019,43(11):72-79.[doi:10.11937/bfyy.20184261]
高温干旱胁迫对牡丹叶PSII功能及光能分配的影响
- Title:
- Effects of High Temperature and Drought Stress on PSII Function and Light Distribution in Peony Leaves With Different Resistance
- Keywords:
- Paeonia suffruticosa; high temperature and drought stress; PSII function; light distribution
- 文献标志码:
- A
- 摘要:
- 以耐旱的“胡红”和对干旱敏感的“洛阳红” 2个不同抗性的牡丹品种为试材，研究了高温干旱协同胁迫下的牡丹叶片PSII功能及光能分配特性，以期为提高干旱胁迫下的牡丹抗性提供理论依据和实践支持。结果表明：高温干旱胁迫引起了2种牡丹叶片PSⅡ初始荧光(F0)、光下叶片初始荧光(F0′)、非光化学猝灭系数(qN)、天线热耗散百分率（Hd）、反应中心非光化学耗散百分率（Ex）的增加和最大荧光(Fm)、可变荧光（Fv）、暗适应最大光化学效率(Fv/Fm)、PSⅡ实际光能转换效率(Fv′/Fm′)、叶绿素荧光光化学猝灭系数(qP)、表观光合电子传递速率(ETR)的降低。胁迫前4 d 2个牡丹品种具有一定的光合保护机制，PSⅡ叶绿素荧光能量分配与对照无显著差异，PSⅡ功能没受到影响。但6 d后光合保护机制受损严重，叶绿素荧光能量分配变化显著，Hd逐渐增加第12天时“胡红”和“洛阳红”分别比对照增加118.9%、153.0%。Pc和ETR逐渐降低，第12天时“胡红”Pc、ETR和“洛阳红”Pc、ETR分别比对照降低70.7%、73.8%和84.9%、97.2%。说明进入PSⅡ反应中心的能量减少，通过天线热耗散的能量增多，PSⅡ功能受抑。胁迫中后期2个牡丹品种间叶绿素荧光能量分配差异显著，抗性较差的“洛阳红”Hd和Ex百分率高于抗性较强的“胡红”，Ex增加造成光合机构更严重的可逆失活及破坏。表明抗性较强的“胡红”比抗性较弱的“洛阳红”，遭受高温干旱胁迫时光抑制程度更低，仍能维持相对较高的PSⅡ实际光能转换效率。
- Abstract:
- Two peony cultivars ‘Huhong’(drought-toleran) and ‘Luoyanghong’(drought susceptible) with different resistance were used to study the PSII function and light distribution characteristics of peony leaves under high temperature and drought stress in order to provide theoretical basis and practical support for improving peony resistance to drought.The results showed that high temperature and drought stress caused increase of PSII initial fluorescence (F0),light-induced leaf initial fluorescence (F0′),non-photochemical quenching coefficient (qN),antenna thermal dissipation percentage (Hd),non-photochemical dissipation percentage (Ex) and decrease of maximum fluorescence (Fm),variable fluorescence (Fv),dark adaptation maximum photochemistry efficiency (Fv/Fm),PSII actual light energy conversion efficiency (Fv′/Fm′),chlorophyll fluorescence photochemistry quenching coefficient (qP),apparent photosynthetic electron transfer rate (ETR).Four days before stress,the two peony had a certain photosynthetic protection mechanism.There were no significant differences in the distribution of chlorophyll fluorescence energy of PSII between two peony cultivars and control.Functions of PSII were not affected.However,after 6 days,photosynthetic protection mechanism was severely damaged,chlorophyll fluorescence energy distribution changed significantly.On the 12th day when Hd increased gradually,‘Huhong’ and ‘Luoyanghong’ increased 118.9% and 153.0% respectively compared with the control.Pc and ETR decreased gradually.On the 12th day,Pc,and ETR of ‘Huhong’ decreased by 70.7%,73.8%.And those of ‘Luoyanghong’ were 84.9% and 97.2% respectively.Thus it could be seen energy entering PSII reaction center decreased,energy dissipation through antenna increased,and PSII function was inhibited.There were significant differences in chlorophyll fluorescence energy distribution between two peony cultivars in the middle and late stages of stress.The percentage of Hd and Ex in ‘Luoyanghong’ cultivars with weak resistance was higher than that in ‘Huhong’ cultivars with strong resistance.The increase of Ex could cause more serious reversible inactivation or even destruction of photosynthetic apparatus.The results showed that ‘Huhong’ with stronger resistance had lower inhibition degree than ‘Luoyang hong’ with weaker resistance under high temperature and drought stress,and could still maintain relatively high PSII actual light energy conversion efficiency.
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备注/Memo
作者简介：刘俊娟（1979-），女，河南汝阳人，硕士，讲师，现主要从事园林植物与观赏园艺教学等研究工作。E-mail：jimiapple@126.com.基金项目：国家自然科学基金资助项目（31101536）；国家重点实验室开放课题资助项目(20120630）；河南科技大学博士科研基金资助项目(09001473)。收稿日期：2019-02-14〖HT〗〖FQ）〗