HAN Shuang,MA Li,PEI Dongli.Study on Tissue Culture Technology of Opisthopappus taihangensis[J].Northern Horticulture,2019,43(06):83-88.[doi:10.11937/bfyy.20182615]
太行菊组织培养技术研究
- Title:
- Study on Tissue Culture Technology of Opisthopappus taihangensis
- Keywords:
- Opisthopappus taihangensis; callus; tissue culture
- 文献标志码:
- A
- 摘要:
- 以太行菊叶片、叶柄、茎尖和茎段为试材，采用MS培养基，在其中添加不同的激素对太行菊的不同外植体进行培养，研究了濒临灭绝的太行菊再生植株的方法。结果表明：太行菊的叶片、叶柄作为外植体诱导出愈伤组织的诱导率分别为44.44%和44.11%，所需时间较长，而茎尖、茎段作为外植体诱导愈伤组织诱导率分别为48.44%和68.75%，培养时间较短。茎段最适合作为诱导愈伤组织的繁殖材料。为了使茎段能更好的诱导出愈伤组织，课题组又对诱导茎段愈伤组织的激素浓度组合进行了研究，A组：MS+6-BA 1.0 mg?L-1+NAA 0.5 mg?L-1+2,4-D 0.1 mg?L-1，B组：MS+6-BA 1.0 mg?L-1+NAA 1.0 mg?L-1+2,4-D 0.1 mg?L-1，C组：MS+6-BA 2.0 mg?L-1+NAA 0.5 mg?L-1+2,4-D 0.1 mg?L-1，D组：MS+6-BA 2.0 mg?L-1+NAA 1.0 mg?L-1+2,4-D 0.1 mg?L-1。试验表明，C组激素浓度组合最适合太行菊茎段的愈伤组织诱导。35 d后愈伤组织不再增加后进行继代培养，45 d后将植株转移至生根培养基1/2MS+NAA 0.12 mg?L-1中进行生根培养。
- Abstract:
- The leaves，petioles，stem tips and stem segments of Opisthopappus taihangensis were used as test materials，and different explants of Opisthopappus taihangensis were cultured with MS medium，and different callus were cultured to grow callus.The method of regenerating plants of the endangered Opisthopappus taihangensis was studied.The results showed that induction rate of callus induction from leaves and petioles of Opisthopappus taihangensis were 44.44% and 44.11%，respectively，which took longer time.Induction rate of callus induction from stem tip and stem segments were 48.44% and 68.75%，respectively and those took less time.Thus，we proved that stem segments were the most suitable materials for callus induction for Opisthopappus taihangensis.In order to effectively induce callus from stem segments，the combination of hormone concentration in callus of stem segments was studied，Group A,MS+6-BA 1.0 mg?L-1+NAA 0.5 mg?L-1+2,4-D 0.1 mg?L-1，Group B,MS+6-BA 1.0 mg?L-1+NAA 1.0 mg?L-1+2,4-D 0.1 mg?L-1，Group C,MS+6-BA 2.0 mg?L-1+NAA 0.5 mg?L-1+2,4-D 0.1 mg?L-1，Group D,MS+6-BA 2.0 mg?L-1+NAA 1.0 mg?L-1+2,4-D 0.1 mg?L-1.The test showed that the concentration of hormone in Group C was the most suitable for callus induction in the stem segment of Opisthopappus taihangensis.After 35 days，the callus was no longer increased and subcultured.After 45 days，the plants were transferred to rooting medium 1/2 MS+NAA 0.12 mg?L-1 for rooting culture.
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备注/Memo
第一作者简介：韩霜（1982-），女，博士，副教授，现主要从事植物光合作用等教学与科研工作。E-mail：htshd_012@163.com.责任作者：裴冬丽（1971-），女，博士，教授，现主要从事分子植物病原体互作等教学与科研工作。E-mail：peidongli@126.com.基金项目：河南省科技厅科技攻关资助项目（162102110161，162102110092）；河南省高等学校重点科研资助项目（17B210010）；国家自然科学基金资助项目（31571997）。收稿日期：2018-10-29