[1]刘柳,南宇航,沙彤芸,等.西瓜种质资源对枯萎病和白粉病的抗性评价[J].北方园艺,2018,42(11):43-49.[doi:10.11937/bfyy.20174762]
　LIU Liu,NAN Yuhang,SHA Tongyun,et al.Evaluation of Watermelon Germplasm Resistant to Fusarium Wilt and Powdery Mildew[J].Northern Horticulture,2018,42(11):43-49.[doi:10.11937/bfyy.20174762]

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西瓜种质资源对枯萎病和白粉病的抗性评价

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷期数: 2018年11 页码: 43-49 栏目: 出版日期: 2018-06-10

Title:: Evaluation of Watermelon Germplasm Resistant to Fusarium Wilt and Powdery Mildew

作者:: 刘柳1; 2; 南宇航1; 2; 沙彤芸1; 2; 张璐澎1; 2; 陈克农1; 2; 王学征1; 2; （1．农业部东北地区园艺作物生物学与种质创制重点实验室，黑龙江哈尔滨 150030； 2.东北农业大学园艺园林学院，黑龙江哈尔滨 150030）

Author(s):: LIU Liu1; 2; NAN Yuhang1; 2; SHA Tongyun1; 2; ZHANG Lupeng1; 2; CHEN Kenong1; 2; WANG Xuezheng1; 2; (1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region), Ministry of Agriculture,Harbin,Heilongjiang 150030；2.College of Horticulture & Landscape Architecture,Northeast Agricultural University，Harbin,Heilongjiang 150030)

关键词:: 西瓜; 枯萎病; 白粉病; 抗病性评价

Keywords:: watermelon（Citrullus lanatus）; Fusarium wilt; powdery mildew; resistant evaluation

DOI:: 10.11937/bfyy.20174762

文献标志码:: A

摘要:: 以44份西瓜种质资源为试材，采取单一接种和复合接种的方法,评价了其对枯萎病和白粉病抗病性，以期为西瓜抗病种质资源评价和筛选提供参考依据。结果表明：单一接种法鉴定出对枯萎病高抗的西瓜种质5份，对白粉病高抗的西瓜种质有2份。复合接种法筛选出对2种病害同时具有抗性的西瓜种质有22份。将复合接种鉴定结果与单一接种鉴定结果进行相关性分析，二者相关性高、准确且科学,复合接种方法可以代替单一接种方法，有利于提高选择效率。

Abstract:: Forty-four watermelon germplasm resources were used as materials,the resistance to Fusarium wilt and powdery mildew were studied by single and composite inoculated approaches,in order to provide the reference basis for evaluating and screening watermelon germplasm resources of disease resistant.The results showed that five watermelon germplasms with high resistance to Fusarium wilt,and two watermelon species resist to powdery mildew were screened by single inoculation identification；22 watermelon germplasm resources for two kinds of disease resistant were screened by compound inoculation at the same time.The compound inoculation tests and single appraisal results were high correlation,accurate and scientific.Therefore,compound inoculation method could replace single method,which was conducive to improve the efficiency of selection.

参考文献/References:

[1]ZHANG Z,ZHANG J,WANG Y,et al.Molecular detection of Fusarium oxysporum f.sp.niveum and Mycosphaerella melonis in infected plant tissues and soil[J]．FEMS Microbiol Lett,2005,249:39-47．

[2]DIENER A C,AUSUBEL F M.Resistance to Fusarium oxysporum 1,a dominant Arabidopsis disease-resistance gene,is not race specific[J].Genetics,2005,171：305-321．

[3]CHEN K S,LIOU T D,CHANG P F L,et al.Selection for resistance of watermelon varieties (lines) to Fusarium wilt and theirgenetic analysis of inheritance[J].Plant Pathol Bull,2003(12):173-180．

[4]SITTERLY W R．Powdery mildew of cucurbits[M]//In：SPENCER D M(ed.) The powdery mildews．New York：Academic Press，1978：359-379．

[5]BORA T，ZAKTAN H，GRE E，et al.Biological control of Fusarium oxysporum f．sp．melonis by wettable powder formulations of the two strains of Pseudomonas putida[J]．Journal of Phytopathology,2004,152(8/9):471-475．

[6]包海青，许勇，杜永臣，等．海南三亚地区葫芦科作物白粉病菌生理小种分化的鉴定[J]．长江蔬菜，2008(1)：49-50.

[7]王登明，张学军，冯炯鑫，等.甜瓜枯萎病接种方法比较试验初报[J].中国瓜菜,2009,22(5):38-39.

[8]徐志豪，寿伟林，黄凯美，等．白粉病菌的生理小种及其对不同基因型甜瓜的致病性[J]．浙江农业学报，1999,11(5)：245-248．

[9]王冰，顾三军，孙小镭.黄瓜苗期复合接种多抗性鉴定方法研究[J].山东农业科学，1997(1)：38-41.

[10]李光，李淑菊，王惠哲.兼抗黄瓜枯萎病、角斑病和黑星病育种材料苗期筛选[J].天津农业科学，2007，13(3)：23-25.

[11]赵国云，王惠哲，李淑菊.黄瓜病害苗期多抗性鉴定方法的评价[J].北方园艺，2008(9)：178-180.

[12]王惠哲，李淑菊.黄瓜枯萎病-白粉病-褐斑病-黑星病多抗性鉴定技术研究[J].中国瓜菜，2010,23(5)：6-8.

[13]宋英，张国芹，刘凤军．不同黄瓜品种对枯萎病，白粉病及霜霉病的抗性鉴定[J]．江苏农业科学，2012,40(7)：121-122．

[14]马鸿艳，祖元刚，栾非时．甜瓜种质资源苗期抗白粉病鉴定[J]．东北农业大学学报，2009,40(12)：18-23．

[15]张学军，李寐华，张龑,等.甜瓜苗期复合接种多抗性方法研究[J].北方园艺，2013(1):15-17.

[16]周凤珍，康国斌，西瓜部分抗枯萎病材料的抗性遗传研究[J].中国西瓜甜瓜，1996(2):17-28.

[17]肖光辉，刘建雄，肖兰异，等.瓠瓜枯萎病抗性导入西瓜的遗传研究与利用[J].湖南农业大学学报,2000,26(2)：90.

[18]顾卫红，杨红娟，马坤，等.西瓜种质资源的抗蔓枯病性鉴定及其应用[J].上海农业学报，2004,20(1):65-67.

[19]张素勤，顾兴芳，张圣平，等.黄瓜对霜霉病和白粉病抗性的相关性研究[J]．中国蔬菜,2007(9):9-11．

[20]许向阳，李景富，王超.番茄枯萎病、叶霉病和根结线虫病苗期多抗性鉴定方法的研究[C]//成都：全国蔬菜遗传育种学术讨论会,2002.

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备注/Memo

第一作者简介：刘柳（1994-），女，硕士研究生，研究方向为西甜瓜遗传育种。E-mail：465824275@qq.com.责任作者：王学征（1978-），女，博士，教授，硕士生导师，研究方向为西甜瓜遗传育种。E-mail：xz6206815@163.com.基金项目：国家自然科学基金资助项目（31372088）；东北农业大学学术骨干资助项目（15XG05）。收稿日期：2018-03-02

更新日期/Last Update: 2018-06-26