LIN Ying,SI Chuncan,FENG Tangkai,et al.Influence of Different Carbon,Nitrogen and Potassium Sources on Phosphate Solubilization by Phosphate-solubilizing Bacteria From Toona sinensis Rhizosphere[J].Northern Horticulture,2018,42(20):1-7.[doi:10.11937/bfyy.20174226]
不同碳氮钾源对香椿根际解磷菌溶磷效果的影响
- Title:
- Influence of Different Carbon,Nitrogen and Potassium Sources on Phosphate Solubilization by Phosphate-solubilizing Bacteria From Toona sinensis Rhizosphere
- Keywords:
- carbon source; nitrogen source; potassium source; phosphate solubilizing microorganisms; phosphate solubilizing ability
- 文献标志码:
- A
- 摘要:
- 以香椿根际土壤为试验材料，采用稀释平板涂布法对香椿根际土样的解磷菌进行了分离，对选取的溶磷圈直径/菌落直径比值最大为2.73的解磷菌XCP9进行了16S rDNA分子鉴定，采用钼锑抗比色法，研究了不同碳源、氮源、钾源对XCP9溶解磷酸三钙效果的影响，以期为香椿微生物磷肥的开发提供一定的参考价值。结果表明：XCP9菌株与伯克氏菌(Burkholderia sp.)具有很高的同源性，不同碳氮钾源对XCP9的溶磷效果有极显著影响，且菌株XCP9的溶磷量与pH之间存在极显著负相关。当以甘露醇为碳源、硫酸铵为无机氮源、氯化钾为钾源时，菌株XCP9的溶磷能力达到最高为122.31 μg·mL-1。
- Abstract:
- In order to provide some reference value for the development of microbial phosphate fertilizer for Toona sinensis.Rhizosphere soil of Toona sinensis was taken as test materials，phosphate-solubilizing bacteria were isolated from Toona sinensis rhizosphere using serial dilution plate method.The strain XCP9 performed maximum ratio of dissolving zone diameter to colony diameter were selected for 16S rDNA molecular identification and the effect of different carbon,nitrogen and potassium sources on phosphate solubilization by XCP9 were studied.The results showed that the strain XCP9 had a very high homology with Burkholderia sp.，the phosphate solubilizing efficiencies of strain XCP9 among different carbon,nitrogen and potassium sources were very significant and there was a very strong significant negative correlation between phosphate solubilizing efficiencies and pH.When using mannitol as carbon source,ammonium sulfate as inorganic nitrogen source,potassium chloride as potassium source.Maximum P solubilization of strain XCP9 achieved with 122.31 μg·mL-1.
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备注/Memo
第一作者简介：林英（1985-），女，博士，讲师，现主要从环境微生物资源的研究与开发等工作。E-mail：jslinying@163.com.基金项目：江西省教育厅科学技术研究资助项目（GJJ151277，GJJ151279）；景德镇市科技资助项目（2015NYZD020）。收稿日期：2018-02-05