[1]张宇君,赵丽丽,陈超,等.燕麦萌发期幼苗对PEG胁迫的生理响应[J].北方园艺,2017,41(21):24-30.[doi:10.11937/bfyy.20171463]
　ZHANG Yujun,ZHAO Lili,CHEN Chao,et al.Physiological Response of Oat Seedlings to PEG Stress at Germination Stage[J].Northern Horticulture,2017,41(21):24-30.[doi:10.11937/bfyy.20171463]
点击复制

燕麦萌发期幼苗对PEG胁迫的生理响应

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年21 页码: 24-30 栏目: 出版日期: 2017-11-15
Title:
Physiological Response of Oat Seedlings to PEG Stress at Germination Stage
作者:
张宇君1; 赵丽丽1; 陈超1; 王普昶2; 李继伟3
1.贵州大学 动物科学学院，贵州 贵阳 550025；2.贵州省草业研究所，贵州 贵阳 550025；3.北京佰青源畜牧业科技发展有限公司，北京 100010
Author(s):
ZHANG Yujun1; ZHAO Lili1; CHEN Chao1; WANG Puchang2; LI Jiwei3
1.College of Animal Science,Guizhou University,Guiyang,Guizhou 550025；2.Guizhou Provincial Institute of Prataculture,Guiyang,Guizhou 550025;3.Beijing Source of Green Animal Husbandry Science and Technology Development Co.Ltd.,Peking 100010
关键词:
燕麦; 干旱胁迫; 生理指标; GGE双标图
Keywords:
oat; drought stress; physiological index; GGE-biplot
DOI:
10.11937/bfyy.20171463
文献标志码:
A
摘要:
以6个燕麦品种为试材，设置不同的PEG胁迫强度，研究各燕麦品种主要生理指标的变化情况，以探究不同燕麦品种萌发期幼苗对PEG胁迫的生理适应机制。结果表明：不同燕麦品种幼苗的丙二醛含量随PEG胁迫强度的加重而不断累积，细胞膜损伤逐渐加重；抗氧化酶活性以及可溶性蛋白质（SP）含量在低浓度胁迫时逐渐增大，维持细胞内的渗透势，保护细胞膜免遭伤害，高浓度胁迫时表现为抑制作用，生理系统破坏严重；结合GGE双标图，发现不同燕麦品种适应PEG胁迫的作用生理指标不同：“甘早燕麦”通过SOD调节；“奥塔燕麦”和“青海444燕麦”由SP和MDA调节适应；“甜燕1号燕麦”通过POD、CAT调节；-0.4 MPa渗透胁迫环境具有较好的区分力和代表性，可作为燕麦生产的最佳胁迫强度和优良燕麦品种筛选的浓度。
Abstract:
In order to investigate the physiological and biochemical effects of different drought stress on Oat seedlings.To explore the mechanism of physiological response of oat seedlings to PEG stress at germination-stage,six oat varieties (Anena sativa L.) were subjected to different drought stress simulated by PEG.The results showed that the MDA content of seedlings of different oat varieties with PEG stress intensity increased the accumulation of cell membrane damage gradually increased;antioxidant enzyme activity and soluble protein content increased at low concentration stress,maintain the osmotic potential of cells,protect the cell membrane from injury,high stress concentration showed inhibitory effect and physiological serious destruction;combined with GGE-biplot,found that different oat varieties to adapt to the role of PEG-6000 stress physiological indicators are different:‘Sweet early oat’ was regulated by SOD;‘Ota oat’ and ‘Qinghai 444 oat’ were regulated by SP and MDA;‘Sweet Yan No.1’ was regulated by POD,CAT.The osmotic stress environment of -0.4 MPa had good regional component and representativeness,which could be used as the best stress intensity of oat production and the concentration of excellent oat varieties.

参考文献/References:

[1]刘建新,王金成,王瑞娟,等.旱盐交叉胁迫对燕麦幼苗生长和渗透调节物质的影响[J].水土保持学报,2012,26(3):244-248.

[2]罗佳,叶鸿程,刘兴林,等.水分胁迫对燕麦生理指标的影响[J].高原山地气象研究,2012,32(3):58-61.
[3]倪香艳,顾军强,钟葵,等.燕麦品种的品质性状及聚类分析[J].中国粮油学报,2016,31(10):18-24.
[4]陈新,宋高原,张宗文,等.PEG-6000胁迫下裸燕麦萌发期抗旱性鉴定与评价[J].植物遗传资源学报,2014,15(6):1188-1195.
[5]张娜,赵宝平,张艳丽,等.干旱胁迫下燕麦叶片抗氧化酶活性等生理特性变化及抗旱性比较[J].干旱地区农业研究,2013,31(1):166-171.
[6]LAWLOR D W.Genetic engineering to improve plant performance under drought:Physiological evaluation of achievements,limitations,and possibilities[J].Journal of Experimental Botany,2013,64(1):83-108.
[7]白向历,齐华,何萍,等.水分胁迫对灌浆期燕麦叶片光合特性的影响[J].园艺与种苗,2006,26(1):25-27.
[8]刘建新,王金成,王瑞娟,等.干旱胁迫下一氧化氮对燕麦幼苗生长和生理特性的影响[J].中国草地学报,2015,37(2):41-45.
[9]刘锦春,CORNELISSEN H C.CO2浓度变化下燕麦对干旱胁迫的生理响应[J].草业科学,2015,32(7):1116-1123.
[10]姚玉波.不同品种亚麻种子萌发期抗旱性鉴定[J].核农学报,2015,29(10):2033-2039.
[11]ASHRAF M.Inducing drought tolerance in plants:Recent advances[J].Biotechnology Advances,2010,28(1):169-183.
[12]贾寿山,朱俊刚,王曙光,等.山西小麦地方品种萌发期的抗旱性[J].华北农学报,2011,26(2):213-217.
[13]李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社,2006.
[14]贾利强.金沙江热河谷造林树种抗旱特性的研究[D].北京:北京林业大学,2003.
[15]赵丽丽,王普昶,陈超,等.持续干旱对金荞麦生长、生理生态特性的影响及抗旱性评价[J].草地学报,2016,24(4):
825-833.
[16]裴帅帅,尹美强,温银元,等.不同品种谷子种子萌发期对干旱胁迫的生理响应及其抗旱性评价[J].核农学报,2014,28(10):1897-1904.
[17]许凯扬,叶万辉,段学武,等.PEG诱导水分胁迫下喜旱莲子草色生理适应性[J].浙江大学学报(农业与生命科学版),2004,30(3):271-277.
[18]吴敏,张文辉,周建云,等.干旱胁迫对栓皮栎幼苗细根的生长与生理生化指标的影响[J].生态学报,2014,34(15):4223-4233.
[19]胡宏远,马丹阳,李双岑,等.水分胁迫对赤霞珠葡萄主要抗旱生理指标及品质的影响[J].灌溉排水学报,2016,35(5):79-84.
[20]代英超,徐奎源,马凯,等.珍稀濒危植物堇叶紫金牛对持续干旱的生理响应[J].生态学报,2015,35(9):2954-2959.
[21]范苏鲁,苑兆和,冯立娟,等.干旱胁迫对大丽花生理生化指标的影响[J].应用生态学报,2011,22(3):651-657.
[22]喻泽莉,何平,张春平,等.干旱胁迫对决明种子萌发及幼苗生理特性的影响[J].西南大学学报(自然科学版),2012,34(2):39-44.
[23]张智猛,戴良香,宋文武,等.干旱处理对花生品种叶片保护酶活性和渗透物质含量的影响[J].作物学报,2013,39(1):133-141.
[24]裴斌,张光灿,张淑勇,等.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J].生态学报,2013,33(5):1386-1396.
[25]赵杰宏,谢升东,王轶,等.GGE双标图在中间香型烟叶特色彰显度分析中的应用[J].中国烟草学报,2013,19(4):28-34.
[26]陈四龙,李玉荣,程增书,等.用GGE双标图分析种植密度对高油花生生长和产量的影响[J].作物学报,2009,35(7):1328-1335.
[27]山仑,陈培元.旱地农业生理生态基础[M].北京:科学出版社,1998:1-17.

相似文献/References:

[1]方 晶.高压电场对干旱胁迫下黄瓜种子萌发的影响[J].北方园艺,2014,38(13):41.
　FANG Jing.Effect of High Voltage Electric Field on Germination of Cucumber Seeds Under Drought Stress[J].Northern Horticulture,2014,38(21):41.
[2]王贵平,王金政,薛晓敏,等.叶面喷施甜菜碱对干旱胁迫下苹果幼树[J].北方园艺,2014,38(12):10.
　WANG Gui-ping,WANG Jin-zheng,XUE Xiao-min,et al.Effect of Spraying Glycinebetaine on Physiological Responses of Apple Young Trees Under Drought Stress[J].Northern Horticulture,2014,38(21):10.
[3]张 爽,赵 纯,董 然,等.槭叶草对PEG-6000模拟干旱胁迫的生理响应[J].北方园艺,2013,37(22):74.
　ZHANG Shuang,ZHAO Chun,DONG Ran,et al.Physiological Responses of Mukdenia rossii Under PEG-6000 Drought Stress[J].Northern Horticulture,2013,37(21):74.
[4]蔡喜悦,陈晓德,刘成,等.外源钙对干旱胁迫下复羽叶栾树幼苗水分及光合特性的影响[J].北方园艺,2013,37(10):58.
　CAI Xi-yue,CHEN Xiao-de,LIU Cheng,et al.Effect of Drought Stress with Exogenous Ca2+on Relative Water Content and the Characteristics of Photosynthesis of Koelreuteria paniculata[J].Northern Horticulture,2013,37(21):58.
[5]梁蕊芳,康利平,徐 龙,等.干旱胁迫对樱桃番茄幼苗叶片生长特性的影响[J].北方园艺,2013,37(23):12.
　LIANG Rui-fang,KANG Li-ping,XU Long,et al.Effect of Drought Stress on Growth Characteristics of Leaves in Lycopersicom esculentum var. cerasiforme Seedlings[J].Northern Horticulture,2013,37(21):12.
[6]王少平,黄超.切花菊‘黄中黄’对干旱胁迫的生理响应[J].北方园艺,2013,37(15):64.
　WANG Shao-ping,HUANG Chao.Physiological Response of Cut Chrysanthemum ‘Huangzhonghuang’ to Drought Stress[J].Northern Horticulture,2013,37(21):64.
[7]董延龙,常缨.干旱胁迫对五种观赏蕨类植物叶绿素荧光特性的影响[J].北方园艺,2013,37(15):66.
　DONG Yan-long,CHANG Ying.Effects of Drought Stress on Chlorophyll Fluorescence Characteristics of Five Species of Ornamental Ferns[J].Northern Horticulture,2013,37(21):66.
[8]岳桦,石喜梅.聚乙二醇模拟干旱胁迫对兔儿伞生理特性的影响[J].北方园艺,2013,37(11):56.
　YUE Hua,SHI Xi-mei.Effects of Drought Stress Simulated by PEG-6000 on Physiological Characteristics of Syneilesis aconitifolia[J].Northern Horticulture,2013,37(21):56.
[9]邹原东,韩振芹,陈秀新,等.干旱胁迫对蓝羊草渗透调节物质和抗氧化酶活性的影响[J].北方园艺,2013,37(23):71.
　ZOU Yuan-dong,HAN Zhen-qin,CHEN Xiu-xin,et al.Effect of Drought Stress on Osmotic Adjustment Substance and Antioxidative Activity of Leymus chinensis[J].Northern Horticulture,2013,37(21):71.
[10]余莉琳,裴宗平,孔静,等.干旱胁迫下四种矿区生态修复植物的抗旱性研究[J].北方园艺,2013,37(12):61.
　YU Li-lin,PEI Zong-ping,KONG Jing,et al.Drought Resistance of Four Plant Species in Ecological Regeneration on Mining Area Under Drought Stress[J].Northern Horticulture,2013,37(21):61.

备注/Memo

第一作者简介：张宇君（1992-），女，硕士研究生，研究方向为牧草种质资源研究及育种。E-mail:zhangyj_92@163.com.

责任作者：赵丽丽（1981-），女，博士，副教授，现主要从事牧草种质资源等研究工作。E-mail:zhaolili_0508@163.com.
基金项目：国家重点研发计划资助项目（2016YFC0502607）；国家自然科学基金资助项目（31560664）；国家科技支撑计划资助项目（2014BAD23B03）；贵州省农业攻关计划资助项目（黔科合NY字[2014]3048号，黔科合支撑[2016]2516号）；贵州大学研究生创新基金资助项目（研农2017002）。
收稿日期：2017-07-13

更新日期/Last Update: 2017-11-13