[1]薛志忠,杨雅华,李海山,等.五十八份菊芋种质资源遗传多样性SRAP分析[J].北方园艺,2017,41(21):31-36.[doi:10.11937/bfyy.20170802]
　XUE Zhizhong,YANG Yahua,LI Haishan,et al.Genetic Diversity of Fifty-eight Jerusalem Artichoke Germplasm Resources Revealed by Sequence Related Amplified Polymorphism(SRAP)[J].Northern Horticulture,2017,41(21):31-36.[doi:10.11937/bfyy.20170802]
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五十八份菊芋种质资源遗传多样性SRAP分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年21 页码: 31-36 栏目: 出版日期: 2017-11-15
Title:
Genetic Diversity of Fifty-eight Jerusalem Artichoke Germplasm Resources Revealed by Sequence Related Amplified Polymorphism(SRAP)
作者:
薛志忠1; 2; 杨雅华1; 2; 李海山2; 3; 张国新1; 2; 刘淑君1; 2
1.河北省农林科学院 滨海农业研究所，河北 曹妃甸 063299;2.河北省盐碱地绿化工程技术研究中心，河北 曹妃甸 063299;3.河北省农林科学院，河北 石家庄 050031
Author(s):
XUE Zhizhong1; 2; YANG Yahua1; 2; LI Haishan2; 3; ZHANG Guoxin1; 2; LIU Shujun1; 2
1.Coastal Agriculture Institute，Hebei Academy of Agriculture and Forestry Sciences，Caofeidian，Hebei 063299;2.Hebei Engineering Research Center for Saline-alkali Land Greening，Caofeidian，Hebei 063299;3.Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang,Hebei 050031
关键词:
菊芋; SRAP; 遗传多样性; 聚类分析
Keywords:
Jerusalem artichoke(Helianthus tuberosus L.); SRAP; genetic diversity; cluster analysis
DOI:
10.11937/bfyy.20170802
文献标志码:
A
摘要:
以58份菊芋种质资源为试材，利用SRAP分子标记技术对其进行遗传多样性研究，为选育耐盐新品种提供参考依据。结果表明：从110对引物中筛选出16对多态性好的引物，共扩增出123条条带，其中多态性条带90条，多态性比率为72.7%。利用NTSYS软件统计分析出菊芋资源间遗传距离为0.01~0.52。应用UPGMA 聚类可将58份菊芋资源划分为三大类群，第一大类群分为4个亚类群。表明利用SRAP技术更能充分揭示菊芋资源间的遗传差异，可作为种质鉴定依据。
Abstract:
Fifty-eight accessions of Jerusalem artichoke were used as materials，SRAP marker was used to analyze genetic diversities,in order to supply references for breeding salt-tolerant new variety.The results showed that 16 primers selected from 110 SRAP-primer were used for amplification and 123 DNA bands were obtained,aincluding 90 polymorphic bands.The percent of polymorphic was 72.7%.The genetic distance indexes of 58 cultivars based on the SRAP data varied from 0.01 to 0.52．Cluster analysis with UPGMA method showed that the 58 resources tested could be divided into three groups．The first group could be divided into four sub groups．The results showed that The SRAP could fully reveal genetic differences for Jerusalem artichoke resources,and could be used in Jerusalem artichoke germplasms identification.

参考文献/References:

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备注/Memo

第一作者简介：薛志忠（1983-），男，河北抚宁人，硕士，助理研究员，现主要从事植物生理及分子生物学等研究工作。E-mail：nksxzz@163.com.

基金项目：河北省自然科学基金资助项目（C2013301065）；河北省科技支撑计划资助项目（152776122D）。
收稿日期：2017-04-17

更新日期/Last Update: 2017-11-13