SUN Zhuo,LI Yadi,JI Jianwei.Response of Chlorophyll Fluorescence Parameters of Tomato Seedlings to Zn Stress[J].Northern Horticulture,2016,40(24):41-44.[doi:10.11937/bfyy.201624011]
番茄幼苗叶绿素荧光参数对锌胁迫的响应
- Title:
- Response of Chlorophyll Fluorescence Parameters of Tomato Seedlings to Zn Stress
- Keywords:
- tomato; Zn stress; chlorophyll fluorescence; chlorophyll content
- 文献标志码:
- A
- 摘要:
- 以番茄种子为试材,采用土壤基质培养方法,设置了不同锌处理浓度(10、50、100、150 μmol?L-1),以 0 μmol?L-1为对照,比较分析了不同浓度锌胁迫下番茄幼苗的根系生长、叶绿素含量以及叶片的叶绿素荧光变化。结果表明:锌处理浓度为10 μmol?L-1的番茄幼苗表现出更好的增长,而高浓度的锌对植物是有害的,可能会导致植物生长发育缓慢以及叶片萎黄病;过量的锌元素使番茄幼苗的光合系统遭到破坏,这是由于锌胁迫降低了番茄幼苗总叶绿素含量,并且提高了丙二醛(MDA)含量。
- Abstract:
- The tomato seeds were used as the test materials,by using soil culture method,different levels of Zn concentrations (10,50,100,150 μmol?L-1) were set,compared with 0 μmol?L-1,four groups of tomato seedling root growth,chlorophyll content and the change of chlorophyll fluorescence in leaf under Zn stress were analyzed.The results showed that,the tomato seedlings would be better when the Zn concentration was 10 μmol?L-1.While the high concentrations of Zn was harmful to plants,which might lead to slow growth and development of plants and the leaf chlorosis.At the same time,Zn excess would make the photosynthetic system of tomato seedlings be destroyed,which was because of the reducing of total chlorophyll content in tomato seedlings caused by Zn stress,and the content of MDA also increased.
参考文献/References:
[1]VALLEE B L,AULD D S.Zinc coordination,function,and structure of zinc enzymes and other proteins[J].Biochemistry,1990,29:5647-5659. [2]李娟,陈杰忠,黄永敬,等.锌营养在果树生理代谢中的作用研究进展[J].果树学报,2011,28(4):668-673. [3]ANDRADE S A L,GRATO P L,SCHIAVINATO S M A,et al.Mazzafera,Zn uptake,physiological response and stress attenuation in mycorrhizal jack bean growing in soil with increasing Zn concentrations[J].Chemosphere,2009,75:1363-1370. [4]JIANG H M,YANG J C,ZHANG J F.Effects of external phosphorus on the cell ultrastructure and the chlorophyll content of maize under cadmium and zinc stress[J].Environmental Pollution,2007,147:750-756. [5]HU H,SPARKS D.Zinc deficiency inhibits chlorophyll synthesis andgasexchange in ‘stuart’ pecan[J].HortScience,1991,26(3):267-268. [6]冯建灿,胡秀丽,毛训甲.叶绿素荧光动力学在研究植物逆境生理中的应用[J].经济林研究,2002,20(4):14-18,30. [7]李建明,潘铜华,王玲慧,等.水肥耦合对番茄光合、产量及水分利用效率的影响[J].农业工程学报,2014,30(10):82-90. [8]ZARCINAS B A,CARWRIGHT B,SPOUNCER L R.Nitric acid digestion and multi-element analysis of plant material by inductively coupled plasma spectrometry[J].Communications in Soil Science and Plant Analysis,1987,18:131-146. [9]INSKEEP W P,BLOOM P R.Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone[J].Plant Physiology,1985,77(2):483-485. [10]HODGES D M,DELONG J M,FORNEY C F,et al.Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds[J].Planta,1999,207(4):604-611. [11]SUBHASH N,MOHANAN C N.Curve-fit analysis of chlorophyllfluorescence spectra:application to nutrient stress detection in sunflower[J].Remote Sens Environ,1997,60:347-356. [12]张国红,眭晓蕾,郭英华,等.施肥水平对日光温室番茄光合生理的影响[J].沈阳农业大学学报,2006,37(3):317-321. [13]丁海东,齐乃敏,朱为民,等.镉\锌胁迫对番茄幼苗生长及其脯氨酸与谷胱甘肽含量的影响[J].中国生态农业学报,2006,14(2):53-55. [14]张阿宏,齐孟文,张晔晖.调制叶绿素荧光动力学参数及其计量关系的意义和公理化讨论[J].核农学报,2008,22(6):909-912.
相似文献/References:
[1]吴晓燕,张丽荣,马建华,等.一种新型微生物制剂对设施番茄土壤微生物数量的影响[J].北方园艺,2013,37(04):42.
[2]刘配军,李景富,许向阳,等.番茄hp1和dg基因高效检测体系的建立[J].北方园艺,2013,37(04):99.
[3]王晓娥,李建国,赵姝荣,等.咸阳市设施番茄根结线虫病的发生与防治[J].北方园艺,2013,37(04):133.
[4]谷婧玥,李景富,许向阳,等.番茄不同果穗间品质性状差异性研究[J].北方园艺,2014,38(12):26.
GU Jing-yue,LI Jing-fu,XU Xiang-yang,et al.Study on Tomato’s Quality and Traits at Different Tassel[J].Northern Horticulture,2014,38(24):26.
[5]吕 涛,于 杰,赵 勇.日光温室越冬栽培番茄适宜品种筛选试验[J].北方园艺,2014,38(12):40.
LV Tao,YU Jie,ZHAO Yong.Selection of Overwintering Tomato Varieties Suitable for Solar Greenhouse in Heilongjiang[J].Northern Horticulture,2014,38(24):40.
[6]李美芹,裴华丽,乔 宁,等.TYLCV外壳蛋白基因植物表达载体构建及转化番茄的研究[J].北方园艺,2014,38(12):84.
LI Mei-qin,PEI Hua-li,QIAO Ning,et al.Study on the Construction of Plant Expression Vector of TYLCV-CP Gene and Its Transferring Into Tomato[J].Northern Horticulture,2014,38(24):84.
[7]李文枫,李景富,许向阳.不同hp基因类型番茄中番茄红素与主要品质性状的差异及相关分析[J].北方园艺,2014,38(13):97.
LI Wen-feng,LI Jing-fu,XU Xiang-yang.Correlation and Difference Analysis Between Lycopene Content and Main Quality Characters of Tomato with Different hp Gene Types[J].Northern Horticulture,2014,38(24):97.
[8]张 浩,李福云,徐志然,等.不同套作模式对温室连作番茄生长产量及土壤微生物与酶活性的影响[J].北方园艺,2014,38(13):46.
ZHANG Hao,LI Fu-yun,XU Zhi-ran,et al.Effects of Different Relay Intercropping Patterns on Continuous Cropping Tomato Growth,Yield and Soil Microflora and Enzymes Activities in Solar Greenhouse[J].Northern Horticulture,2014,38(24):46.
[9]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2014,38(11):158.
YANG Hui-fang,LIANG Xin-an,CHANG Jie-tian,et al.Effects of Foliar Spraying of Selenium Fertilizer on Selenium Enrichment of Different Vegetables and Yield[J].Northern Horticulture,2014,38(24):158.
[10]祁连弟,管建慧,其日格,等.不同比例牛羊粪便栽培基质对温室番茄苗期生长的影响[J].北方园艺,2013,37(22):47.
QI Lian-di,GUAN Jian-hui,QI Ri-ge,et al.Effects of Different Nursery Substrates with Cow and Sheep Dung on the Seedlings Growth of Tomato in Solar Greenhouse[J].Northern Horticulture,2013,37(24):47.
备注/Memo
第一作者简介:孙卓(1989-),男,硕士研究生,研究方向为农业生物环境智能化检测与控制系统。E-mail:461140401@qq.com.责任作者:纪建伟(1963-),男,博士,教授,博士生导师,现主要从事智能化检测与自动控制技术等研究工作。基金项目:2013年辽宁省教育厅优秀人才支持计划资助项目(LR2013033)。