SUN Xiaomei,WANG Sisi,YANG Hongguang,et al.Antioxidant Enzymes Activity of Paeonia lactiflora During Seed Germination[J].Northern Horticulture,2015,39(14):53-55.[doi:10.11937/bfyy.201514016]
芍药种子萌发过程中抗氧化酶活性变化规律的研究
- Title:
- Antioxidant Enzymes Activity of Paeonia lactiflora During Seed Germination
- Keywords:
- Paeonia lactiflora; seed germination; SOD activity; POD activity; CAT activity
- 文献标志码:
- A
- 摘要:
- 以芍药种子为试材，测定其萌发过程中抗氧化酶活性的动态变化，分析探究芍药种子萌发过程中抗氧化酶活性变化规律。结果表明：超氧化物歧化酶（SOD）活性和过氧化物酶（POD）活性与种子萌发呈负相关，过氧化氢酶（CAT）活性与萌发呈正相关且处于缓慢上升状态。在吸胀12 h左右时芍药种子开始萌发，且SOD活性较POD活性、CAT活性更早发挥作用。
- Abstract:
- Using the herbaceous Paeonia lactiflora seeds as the test materials，the dynamic changes of the activity of antioxidant enzymes in the process of its germination were determined,the relationship between the Paeonia lactiflora seeds germination process and the activity of antioxidant enzymes were analyzed and explored.The results showed that superoxide dismutase (SOD) activity and peroxidase (POD) activity were negatively correlated with seed germination，catalase (CAT) activity and germination were positively correlated and in the rising state slowly.Paeonia lactiflora seed began to germinate in about 12 hours’ imbibition，and SOD worked earlier than POD and CAT.
参考文献/References:
[1]杨洋.芍药种子萌发的生物学特性及破眠技术的研究[D].哈尔滨：东北林业大学,2009. [2]刘心民,程逸远,张霁,等.牡丹种子萌发特性与播种繁殖技术研究进展[J].河南林业科技,2005,25(4)：38-41. [3]宋春花,康晓飞,郭先锋,等.不同处理对芍药种子发芽的影响[J].林业实用技术,2010(2)：49-50. [4]孙晓梅,杨盼盼,杨宏光,等.芍药种子破除休眠方法研究[J].辽宁农业科学,2013(4)：20-22. [5]陈彩霞,王九龄,智信.国内外红松种子休眠及催芽问题研究动态[J].世界林业研究,1997,10(5)：3-9. [6]BOGDANOVIC J,RADOTIC K,MITROVIC A.Changes in activities of antioxidant enzymes during Chenopodium murale seed germination[J].Biologia Plantarum,2008,52(2)：396-400. [7]伍贤进,宋松泉,田向荣.玉米种子吸胀萌发过程中抗氧化酶活性的变化[J].吉林农业大学学报,2004,26(1)：6-9. [8]覃柳燕,唐美琼,梁莹,等.不同温度贮藏后山豆根种子酶活性与发芽率相关性研究[J].种子,2011,30(11)：8-10. [9]DUCIC T,LIRIC-RAJLIC I,MITROVIC A,et al.Activities of antioxidant systems during germination of Chenopodiumn rubrum seeds[J].Biologia Plantarum,2003,47(4)：2115-2121. [10]MCCORD J M，FRIDOVICH I.Superoxide dismutase：an enzymatic function for erythrocuprein (hemocuprein)[J].Journal of Biological Chemistry,1969,244：6049-6055. [11]叶常丰,戴心维.种子学[M].北京：中国农业出版社,1994. [12]赵永华,杨世林.西洋参种子休眠解除与磷酸戊糖途径关系的研究[J].中草药,2001,32(3)：259-261. [13]王艳华,高述民,李风兰,等.大山樱种子休眠机理的探讨[J].种子,2005,24(5)：12-16. [14]纪瑛,胡虹文.种子生物学[M].北京：化学工业出版社,2009：97. [15]张圆,王普昶,王慧慧,等.刺槐种子萌发过程中的水分与抗氧化酶活性变化[J].种子,2014(9)：33.
相似文献/References:
[1]傅巧娟,李春楠,陈一,等.我国一串红的研究进展[J].北方园艺,2013,37(04):192.
[2]石秋梅,邓翻云,吴敏言,等.罗布麻和大叶白麻种子萌发及幼苗生长耐盐性研究[J].北方园艺,2014,38(12):128.
SHI Qiu-mei,DENG Fan-yun,WU Min-yan,et al. Study on Salt Tolerance of Apocynum venetum Linn.and Poacynum hendersonii(Hook.f.) Woodson at Stages of Seed Germination and Seedlings Growth[J].Northern Horticulture,2014,38(14):128.
[3]金 兰,陈 志.青海肋果沙棘和中国沙棘种子萌发特性比较研究[J].北方园艺,2014,38(13):39.
JIN Lan,CHEN Zhi.Comparative Study on Germination Characteristics of Hippophae neurocarpa and Hippophae rhamnoides Seeds[J].Northern Horticulture,2014,38(14):39.
[4]梁 芳,衣采洁.不同浸种处理对小冠花及蛇鞭菊种子萌发的影响[J].北方园艺,2014,38(11):62.
LIANG Fang,YI Cai-jie.Infulence of Different Processing Methods on the Seed Germination of Coronilla varia and Liatris spieata [J].Northern Horticulture,2014,38(14):62.
[5]崔兴国.不同浓度碳酸氢钠胁迫对圆叶牵牛种子发芽的影响[J].北方园艺,2014,38(11):74.
CUI Xing-guo.Effect of Different Concentration of NaHCO3 Stress on Seed Germination of Pharbitis purpurea(L.)Voigt[J].Northern Horticulture,2014,38(14):74.
[6]常青山,张利霞,郑轶琦,等.盐胁迫下钙离子对苜蓿种子萌发的影响[J].北方园艺,2014,38(10):64.
CHANG Qing-shan,ZHANG Li-xia,ZHENG Yi-qi,et al.Effects of Ca2+?on Seed Germination Characteristics of Medicago sativa Under Salt Stress[J].Northern Horticulture,2014,38(14):64.
[7]张 娇,张大治,马 艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014,38(14):65.
ZHANG Jiao,ZHANG Da-zhi,MA Yan,et al.Effect of NaCl Simulation of Salt Stress on Seed Germination and Seedling Growth of Ammopiptanthus mongolicus[J].Northern Horticulture,2014,38(14):65.
[8]杨 飞,郭海波,吴 菊,等.NaCl胁迫对白菜种子萌发及幼苗生长的影响[J].北方园艺,2014,38(01):26.
YANG Fei,GUO Hai-bo,WU Ju,et al.Effect of NaCl Stress on the Seed Germination and Seedling Growth of Chinese Cabbage Cultivars[J].Northern Horticulture,2014,38(14):26.
[9]张雷,李永洁,王雅茹,等.海水胁迫对半枝莲种子萌发的影响[J].北方园艺,2013,37(13):69.
ZHANG Lei,LI Yong-jie,WANG Ya-ru,et al.Effect of Seawater Stress on Scutellaria barbata Seed Germination[J].Northern Horticulture,2013,37(14):69.
[10]何会流.外源水杨酸对盐胁迫下凤仙花种子萌发特性的影响[J].北方园艺,2013,37(13):75.
HE Hui-liu.Effect of Exogenous Salicylic Acid on the Seeds Germination Characters of Impatiens balsamina L.Under NaCl Stress[J].Northern Horticulture,2013,37(14):75.
备注/Memo
第一作者简介：孙晓梅（1970-），女，辽宁沈阳人，博士，教授，现主要从事园林植物种质资源与育种等研究工作。E-mail：xiaomei7280@126.com.基金项目：国家自然基金资助项目（31240028，31470696）。