WU Yaqiong,LIU Jing,WANG Guibin,et al.Tissue Culture and Rapid Propagation of Acer rubrum[J].Northern Horticulture,2016,40(20):97-102.[doi:10.11937/bfyy.201620025]
美国红枫的组织培养与快繁技术
- Title:
- Tissue Culture and Rapid Propagation of Acer rubrum
- Keywords:
- Acer rubrum; tissue culture; rooting
- 文献标志码:
- A
- 摘要:
- 以美国红枫腋芽茎段为外植体,采用组织培养方法,研究了不同激素配比对美国红枫无菌体系的建立、启动、增殖、壮苗培养、试管苗生根移栽的影响。结果表明:带腋芽茎段最佳消毒方式为以70%酒精浸泡30 s,1%升汞消毒6 min,成活率和抽芽率最高,分别达到75.22%和95.13%;NAA对启动培养的影响大于6-BA,接种于培养基MS+NAA 0.05 mg?L-1+6-BA 0.5 mg?L-1中的外植体诱导率最高,为62.80%,培养基及激素的最佳配比为NN69+2,4-D 0.1 mg?L-1+TDZ 0.3 mg?L-1,诱导率达到80.21%;增殖培养阶段的最佳培养基为NN69+NAA 0.1 mg?L-1+TDZ 0.3 mg?L-1,增殖系数为4.2;MS+2,4-D 0.1 mg?L-1+6-BA 0.5 mg?L-1能有效促进小苗的生长;生根培养最适培养基MS+NAA 0.1 mg?L-1+2,4-D 0.5 mg?L-1,生根率达99.8%,生根条数达7.9,根长达6.5 cm;练苗后移栽到蛭石+珍珠岩(1∶1)基质中,移栽成活率为75.2%。
- Abstract:
- Taking Acer rubrum as material, using transformation technology,the whole process from the sterile system establishment,start up cultivation,breeding,seedling cultivation,root culture and hardening-seedling transplanting were discussed by different homone proportion.The results showed that the best disinfection way of the stem with bud explants was disinfection by 70% alcohol immersion 30 seconds+6 minutes disinfection by 1% mercuric chloride.The highest survival rate and sprout rate were 75.22% and 95.13%.Adopting orthogonal test,medium and hormone ratio test to start training.The former result showed that the influence of NAA was greater than 6-BA,MS+NAA 0.05 mg?L-1+6-BA 0.5 mg?L-1 had the highest start rate of 62.80%.The latter result showed NN69+2,4-D 0.1 mg?L-1+TDZ 0.3 mg?L-1 had the highest start rate of 80.21%.The best medium for multiplication culture was NN69+NAA 0.1 mg?L-1+TDZ 0.3 mg?L-1,the multiplication factor was 4.2.The MS+2,4-D 0.1 mg?L-1+6-BA 0.5 mg?L-1 could effectively promote the growth of test-tube seedlings.In root culture stage,the optimal culture medium was MS+NAA 0.1 mg?L-1+2,4-D 0.5 mg?L-1.The rooting effect was best with root rate 99.8%,root number 7.9,root length 6.5 cm.Amounts of elements benefits test-tube plant rooting.After the culture-bottle seedlings were transplanted into the matrix composed of vermiculite and perlite (1∶1,V∶V),the transplanting survival rate was up to 75.2%.
参考文献/References:
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备注/Memo
第一作者简介:吴雅琼(1992-),女,硕士研究生,研究方向为森林培育。E-mail:347470439@qq.com.责任作者:汪贵斌(1970-),男,博士,教授,研究方向为森林培育。E-mail:gbwang@njfu.edu.cn.基金项目:江苏高校优势学科建设工程资助项目(PAPD)。