WANG Liying,QIAO Jun,SHI Yao,et al.Research Progress of Isolated Microspore Culture of Eggplant[J].Northern Horticulture,2015,39(13):190-194.[doi:10.11937/bfyy.201513053]
茄子游离小孢子培养研究进展
- Title:
- Research Progress of Isolated Microspore Culture of Eggplant
- 文章编号:
- 1001-0009（2015）13-0190-05
- Keywords:
- eggplant; microspore; isolated; differentiation; regeneration
- 分类号:
- S 641.103.6
- 文献标志码:
- A
- 摘要:
- 单倍体育种是进行种质资源创新并快速固定优良性状的有效方法，而游离小孢子培养技术是获得单倍体的最佳途径。现从取材、预培养、游离、分化和再生5个方面综述了茄子游离小孢子培养胚胎发生及植株再生的影响因素，并针对茄子游离小孢子培养中存在的一些问题提出了今后的研究方向。
- Abstract:
- Haploid breeding was an effective method for germplasm innovation and good traits fixtion.Haploid was obtained mainly by the method of isolated microspore culture.This paper reviewed the factors affecting the microspore embryogenesis and plant regeneration of eggplant，such as materials,pretreatment，microspores isolation,differentiation and plant regeneration and discussed the future research direction according to the existing problems in the isolated microspore culture of eggplant.
参考文献/References:
[1]Sharma P,Rajam M V.Genotype,explant and position effects on organogenesis and somatic embryogenesis in eggplant [J].Joumal of Experimental Botany,1995,46:135-141.[2]余凤群,刘后利.供体材料和培养基类型对甘蓝型油菜小孢子胚状体产量的影响 [J].华中农业大学学报,1995,14(4):327-331.[3]牛永超,李加纳,殷家明.基因型和处理温度对芥菜型油菜游离小孢子培养的影响 [J].西南大学学报,2010,32(8):23-28.[4] Raina S K,Iyer R D.Differentiation of diploid plants from pollen callus in anther cultures of Solanum melongena L [J].Pflanzenzüchtg,1973,70:275-280. [5]张子君,徐矿红,田云,等.辣椒花药培养诱导胚状体成苗 [J].辽宁农业科学,2000(4):43-45.[6]官春云.油菜小孢子培养和双单倍体育种研究 [J].作物学报,1995,21(6):665-670.[7] Kristiansen K,Andersen S B.Effects of donor plant temperature，photoperiod，and age on anther culture response of Capsicum annuum L [J].Euphytica,1993,67(1):105-109.[8]Maheshwari S C,Tyagi K,Malhotra,et al.Induction of haploidy from pollen grains in Angiosperms the current status [J].Theor Appl Genet,1980,58:193-206.[9]袁亦楠,朱德蔚,连勇,等.番茄游离小抱子培养形成胚状体的初步研究 [J].农业生物技术学报,1999,7(1):85-88.[10]宋彦平,申书兴,王彦华,等.茄子游离小孢子培养获得愈伤组织的研究 [J].河北农业大学学报,2007(3):32-35.[11]佟曦然,顾淑荣,朱至清,等.茄子游离小孢子培养中小孢子发育的细胞学观察 [J].农业生物技术学报,2007,15(5):861-866.[12]Sibi M R,de Vaulx D，Chambonnet D.Obtention de plantes haploids par androgenesis in vitro chezle piment(Capsicum annuum L.) Ann.Amelior [J].Plants,1979,29:583-606.[13]连勇,刘富中,陈钰辉,等.茄子体细胞杂种游离小孢子培养获得再生植株 [J].园艺学报,2004,31(2):233-235.[14]董艳荣,龚义勤.茄果类蔬菜花药和花粉培养研究进展 [J].长江蔬菜,2001(5):30-32.[15]Miyoshi K.Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L.) [J].Plant Cell Rep,1996(15):391-395.[16]Rotino G L,Falavigna A,Restaino F.Production of anther-derived plantlets of eggplant [J].Capsicum Newsl,1987(6):89-90.[17]Duncan E J,Heberl E.Effect of temperature shock on nuclear phenomena in microspores of Nicotiana tabacum and consequently on plantlet production [J].Protoplasma,1976,90(3):173-177.[18]Burgin J P,Nitcsh J P.Obtention de Nicotiana haploids apartir determine cultivars [J].Ann Physiol Veg,1967(9):377-382.[19]Barinova I,Clement C,Martiny L,et al.Regulation of developmental pathways in cultured microspores of tobacco and snapdragon by medium pH [J].Planta,2004，219:141-146.[20]朱朝辉,钟开勤,黄建都,等.茄子游离小孢子培养初探 [J].热带作物学报,2011,32(10):1883-1887.[21]王凤华,李光远,陈双臣,等.茄子愈伤组织诱导及悬浮细胞培养 [J].Agricultural Science and Technology，2013,14(9):1220-1223,1243.[22]范适.茄子小孢子培养研究 [D].长沙:湖南农业大学,2003.[23]张玉苗.不同茄子材料小孢子脱分化及影响因子研究 [D].北京:中国农业科学院研究生院,2011.[24]Ku S J.Plantlets from isolated pollen cultures of eggplant(Solanum melongena.L) [J].Acta Botanica Sinica,1979,21(1):30-35.[25]李华,孙振英,连勇,等.茄子小孢子热激效应和发育潜能的细胞学分析 [J].种子,2008,27(10):1-5.[26]马欣,申书兴,连勇,等.茄子花药和游离小孢子培养技术研究进展 [J].长江蔬菜,2006(7):39-41.[27]王烨,张宝玺,连勇,等.不同预处理对辣椒小孢子存活率的影响 [J].中国蔬菜,2004(4):4-6.[28]周广栋,王秀峰,谢冰,等.番茄花药离体培养中低温预处理对小孢子发育的影响 [J].中国农学通报,2005,21(2):192-195.[29]Johansson L,Eriksson T.Effects of carbon dioxide in anther culture [J].Physiol Plant,1983,59:397-403.[30]Kohlenbach H W,Wernicke W.Investigations on the inhibitory effect of agar and the function of active carbon in anther culture [J].F K Z Pflanzenphysiol, 1978,86:463-472.[31] Carlos J,Dias D S.Effects of activated charcoal on Brassica oleracea microspore culture embryogenesis [J].Euphytica,1999,108:65-69.[32]李春玲,邓晓梅,朱至清,等.甜(辣)椒未成熟小孢子直接诱导萌发成植株的方法 [P].2011,发明专利.
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备注/Memo
第一作者简介：王利英（1971-），男，硕士，副研究员，现主要从事茄果类育种等研究工作。E-mail:sxdtqj886@126.com.责任作者：李素文（1965-），女，本科，研究员，现主要从事科研管理工作。E-mail：tjvri@126.co基金项目：天津市农业科学院院长基金资助项目（12007）；天津市农业科技成果转化与推广资助项目（201304020）。