MO Yun-cai,ZENG Ling-jie,FENG Hong-yao,et al.Effect of Enhanced Ultraviolet-B Radiation on Protective Enzymes in Dendrobium officinale Kimura et Migo Seedling Leaves[J].Northern Horticulture,2015,39(05):170-173.[doi:10.11937/bfyy.201505049]
增强UV-B辐射对铁皮石斛保护酶的影响
- Title:
- Effect of Enhanced Ultraviolet-B Radiation on Protective Enzymes in Dendrobium officinale Kimura et Migo Seedling Leaves
- Keywords:
- Dendrobium officinale Kimura et Migo; enhanced UV-B radiation; protective-enzyme activation; MDA
- 文献标志码:
- A
- 摘要:
- 以铁皮石斛品种“云南红杆”为试材，研究了5.1 μW/cm2(L1)、10.3 μW/cm2(L2)和15.6 μW/cm2(L3)3个强度的UV-B辐射处理下，铁皮石斛叶片保护酶活性及MDA含量的变化差异。结果表明：在整个辐射处理期间，L1处理叶片的SOD活性总体高于CK，幅度波动不明显；L2和L3处理的SOD活性总体趋势呈先升高后降低的变化趋势。3个处理组的POD均呈现出先升高后降低的趋势，之后升高再降低。L1处理的CAT活性先缓缓下降，然后逐渐上升，最后趋于平稳；L2和L3的波动幅度较大，最后都明显降低。随着辐射时间的延长，叶片中MDA含量明显增加，如L2和L3。而L1处理下叶片中MDA含量与CK相比，含量变化不大。分析表明，UV-B辐射造成抗氧化系统的变化，可能是由于UV-B辐射下活性氧积累导致的。
- Abstract:
- Taking ‘Yunnan Honggan’ which was a type of Dendrobium officinale Kimura et Migo as test material，the study was done to reveal the changes of protective-enzyme activation and MDA in Dendrobium officinale Kimura et Migo seedling leaves，exposing to three kinds radiations：ultraviolet-B radiation(UV-B)of 5.1 μW/cm2，10.3 μW/cm2 and 15.6 μW/cm2.The results showed that the activities of SOD in L1 treatment were higher than that of CK and with a small fluctuation.The overall trend of the SOD in L2 and L3 treatments were rising and then declining.The activities of POD of L1，L2 as well as L3 showed a rising and declining trend in turn.The activity of CAT in L1 treatment showed a result as below：at first increased slowly，then decreased gradually，and finally tended to be smooth and stable；the results in L1 and in L3 treatments were different from that in L1：firstly with a big fluctuation，and finally with an obvious decline.With radiated time going on，the contents of MDA in the leaves increased obviously，such as in L2 and in L3 treatments.However，comparing with CK，the content of MDA in L1 treatment had a small fluctuation.The analysis indicated that the accumulation of Reactive Oxygen Species(ROS) induced by UV-B radiation may influence the biosynthesis of antioxidant systems.
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备注/Memo
第一作者简介：莫运才（1991-），男，广东湛江人，硕士研究生，研究方向为中药质量评价。E-mail:1210309308@qq.com. 责任作者：曾令杰（1970-），男，湖南人，博士，教授，研究方向为中药材GAP种植与中药质量研究。E-mail:598479380@qq.com. 基金项目：广东省科技计划重点资助项目（2012A030100012)。