MENG Heng-ling,ZHANG Wei,LU Bing-yue,et al.Effect of Copper Stress on Physiological Indexes in Lonicera japonica Seedling[J].Northern Horticulture,2015,39(05):167-169.[doi:10.11937/bfyy.201505048]
铜胁迫对金银花幼苗生理指标的影响
- Title:
- Effect of Copper Stress on Physiological Indexes in Lonicera japonica Seedling
- Keywords:
- Lonicera japonica; copper stress; physiological index
- 文献标志码:
- A
- 摘要:
- 以金银花品种“金花3号”为试材，分别采用不同浓度的CuSO4·5H2O溶液处理，研究金银花在不同铜浓度胁迫下的丙二醛（MDA）含量、脯氨酸含量、可溶性糖含量、超氧化物歧化酶（SOD）活性和过氧化物酶（POD）活性的变化情况，探讨重金属铜对“金花3号”生长的影响。结果表明：铜胁迫处理对金银花生长具有低促高抑的作用，随着营养液中铜含量的增加及胁迫时间的延续，抗氧化物酶SOD、POD活性随处理浓度的变化呈一定的变化规律，低浓度处理（100、200 mg/L）呈上升趋势，高浓度处理（400、800 mg/L）呈下降趋势。MDA含量变幅较小，脯氨酸含量先升高后降低，可溶性糖含量先降低后升高，脯氨酸和可溶性糖间存在补偿作用。该研究认为，金银花在铜元素含量低于25 mg/L的环境中能够正常生长，属于耐重金属污染较强的植物。
- Abstract:
- Taking Lonicera japonica“Jinhua No.3”as materials，the variation of physiological indexes in L.japonica，such as the contents of malondialdehyde(MDA)，proline，soluble sugar，and the activities of superoxide dismutase(SOD) and peroxidase (POD) under various concentrations copper stress were studied.The results showed that the copper damage phenomenon of L.japonica was present with the accumulating copper in Hoagland nutrient solution.The activities of SOD and POD demonstrated change rules with different copper concentrations that were raise under low concentrations treated(100 mg/L and 200 mg/L) and reducing under high concentrations treated (400 mg/L and 800 mg/L).And MDA content was reducing in different degree after copper stress 8 days.Proline content rose rapidly first then reduced.The soluble sugar content was lower first then rising after 6 days.Between the contents of proine and soluble sugar had compensating effect.L.japonica could normally grow that copper content was less than 25 mg/L environment. L.japonica was a good plant for resistance copper stress.
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备注/Memo
第一作者简介：孟衡玲(1981-)，女，云南宣威人，博士，讲师，现主要从事药用植物资源的开发与利用等研究工作。E-mail：menghengl@163.com. 责任作者：刘艳红（1968-），女，云南个旧人，博士，教授，现主要从事云南特色植物资源的开发与利用等研究工作。E-mail：kidliu1968@126.com. 基金项目：云南省科技厅应用基础研究资助项目（2012FB174）。