[1]丁晓雯,程 洁,吴玉川,等.牡丹芽自由态IAA的变化及其与ARP基因的关系[J].北方园艺,2015,39(03):92-95.[doi:10.11937/bfyy.201503028]
　DING Xiao-wen,CHENG Jie,WU Yu-chuan,et al.Changes of Free IAA and Relationship Between PsARP in Tree Peony[J].Northern Horticulture,2015,39(03):92-95.[doi:10.11937/bfyy.201503028]
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牡丹芽自由态IAA的变化及其与ARP基因的关系

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年03 页码: 92-95 栏目: 出版日期: 2015-02-15
Title:
Changes of Free IAA and Relationship Between PsARP in Tree Peony
作者:
丁晓雯1; 程 洁1; 吴玉川2; 周荣全3; 劳俊峰1; 穆 平1
（1.青岛农业大学 农学与植保学院，山东省旱地作物水分高效利用创新团队，山东 青岛 266109；2.山东省济宁市农业局,山东 济宁272100；3.山东省定陶县冉堌镇农技站，山东 定陶 274100）
Author(s):
DING Xiao-wen1; CHENG Jie1; WU Yu-chuan2; ZHOU Rong-quan3; LAO Jun-feng1; MU Ping1
(1.College of Agronomy and Plant Protection，Qingdao Agricultural University，Innovation Team of Drought Resistance in Shandong Province，Qingdao,Shandong 266109；2.Jining Bureau of Agriculture，Jining,Shandong 272100；3.Rangu Agrotechnical Station，Dingtao,Shandong 274100)
关键词:
牡丹; 休眠解除; 自由态IAA; 荧光定量
Keywords:
Paeonia suffroticosa; dormancy release; free IAA; real-time PCR
DOI:
10.11937/bfyy.201503028
文献标志码:
A
摘要:
以牡丹品种‘鲁荷红’为试材，在不同低温处理下研究PsARP基因的表达变化及花芽内源IAA的含量变化，以阐明牡丹花芽休眠解除期间自由态IAA的变化及与PsARP基因表达之间的关系。结果表明：在低温处理前期，随着低温时间的增加，自由态IAA的含量不断降低，达花芽休眠解除临界点时IAA含量最低。在低温处理的后期，随着低温时间的增加，自由态IAA的含量呈下降趋势，但仍保持较高的含量，12 d和18 d之间是个转折点。实时荧光定量分析表明，低温处理前期，PsARP基因的表达量逐渐增加，且在12 d时达到最高点；低温处理第二阶段，PsARP基因的表达量快速下降，30 d时快速下降至最高表达量的50%。内源自由态IAA含量的变化可以反映花芽休眠解除的状态。
Abstract:
Using Paeonia suffroticosa‘Luhehong’as plant materials，the expression pattern of PsARP and changes of free IAA were conducted under different chilling treatments.In order to reveal the changes of free IAA and the relationship between PsARP during dormancy release by chilling treatment in tree peony.The results showed that during the first 12 days of chilling treatment，the content of free IAA continuously reduced with the increase of chilling treatment.The lowest point was in accordance with the dormancy release of tree peony.In the later part of chilling treatment，although a reduction situation was also maintained，a relatively high level of content of free IAA was detected.Chilling treatment between 12 days and 18 days was a key point.By Real-time PCR，it showed that the expression of PsARP was enhanced and continuously increased in the buds of tree peony during the first step of dormancy release by chilling treatment，reaching the highest value during 12 days chilling treatment.At the second part of chilling treatment，the expression level of PsARP reduced to 50% than that of the highest level.The variation of free IAA could reflect the dormancy release degree of the flower bud.

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备注/Memo

第一作者简介：丁晓雯（1990-），女，山东枣庄人，硕士研究生，研究方向为植物分子育种。E-mail:709939160@qq.com.责任作者：穆平（1971-），男，山东博兴人，博士，教授，现主要从事作物遗传育种等研究工作。E-mail：muping@qau.edu.cn.基金项目：山东省农业良种工程资助项目；山东省抗旱节水创新团队专项资金资助项目。

更新日期/Last Update: 2015-03-03