PAN Mei,FU Rui-kan,HUANG Sai,et al.Screening Study on Proliferation Medium of Micropropagation Technique for Callicarpa nudiflora[J].Northern Horticulture,2014,38(04):96-99.
药用裸花紫珠微繁技术增殖培养基筛选研究
- Title:
- Screening Study on Proliferation Medium of Micropropagation Technique for Callicarpa nudiflora
- 文章编号:
- 1001-0009(2014)04-0096-04
- Keywords:
- Callicarpa nudiflora; clustered buds; proliferation culture; screening
- 分类号:
- Q 943.1
- 文献标志码:
- A
- 摘要:
- 以裸花紫珠丛生芽为外植体，在光照时间9 h/d,光照强度1 500 lx,温度(26±2)℃,pH 5.8的培养条件下，研究比较了不同无机盐量MS培养基、不同植物生长调节剂种类及配比、蔗糖浓度、培养周期等因素对裸花紫珠生长状况的影响，以期筛选出裸花紫珠微繁技术最佳增殖培养基。结果表明：丛生芽〖JP4〗继代增殖的最佳配方为:MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+蔗糖30 g/L，30 d的增殖系数达到10以上,丛生芽长势好。
- Abstract:
- Taking the clustered buds of Callicarpa nudifloraas the explant，the effect of inorganic salt types and concentrations of plant growth regulators，sucrose concentration，culture cycle and other factors on growth ofCallicarpa nudiflorawere studied under the conditions as light time 9 h/d，light intensity of 1 500 lx,temperature of (26±2)℃ and pH 5.8,in order to screen the optimum proliferation medium of micropropagation technique.The results showed that the best formula for clustered buds subculture proliferation was MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+sucrose 30 g/L，proliferation coefficient was over 10 in 30 d and clustered buds growth were potential.
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备注/Memo
第一作者简介：潘梅（1962-），女，广西人，高级园艺师，现主要从事植物组织培养等研究工作。E-mail：panmei200@sina.com.