LI Shu-li.Effect of Temperature and Light Condition on Explants Browning of Populus×euramericanacv.Zhonghuahongye[J].Northern Horticulture,2014,38(04):94-95.
温度及光照条件对中华红叶杨外植体褐变的影响
- Title:
- Effect of Temperature and Light Condition on Explants Browning of Populus×euramericanacv.Zhonghuahongye
- 文章编号:
- 1001-0009(2014)04-0094-03
- Keywords:
- Populus×euramericana?cv Zhonghuahongye; temperature; light; browning; effect
- 分类号:
- S 792.11
- 文献标志码:
- A
- 摘要:
- 以中华红叶杨茎段为外植体,研究了温度及光照条件对其褐变的影响。结果表明:外植体低温预处理与简单培养基培养相结合,抑制褐化效果好;培养初期进行暗培养能有效降低褐化率,以暗培养10 d为宜;温度对褐化率也有影响,在一定范围内,温度越高,褐化越严重,低温可较好地减轻褐化;光照强度1 000~3 000 lx对褐化率的影响不大。
- Abstract:
- Taking the stem of Populus×euramericanacv.Zhonghuahongye?as explant,the effect of temperature and light condition on explants browning of it were studied.The results showed that low temperature pretreatment combined with simple culture medium culture could decrease browning rate and had a good browning decreasing effect;dark culture decreased browning rate effectively at the first stage and ten days was an optimum;temperature also affected browning rate,within a certain range the higher the temperature was,the more serious the browning was,and low temperature lightened browning;the light intensity in 1 000~3 000 lx could not cause an obvious effect for the browning rate.
参考文献/References:
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备注/Memo
作者简介:李树丽(1974-),女,硕士,讲师,现主要从事植物组织培养的教学与科研工作。