DU Bao-wei,FANG Qing,HU Yue-hua,et al.Selection of the Suitable Proliferation Medium in the Rapid Propagation System of ‘Peach Dwarf Yunong No.1 Rootstock’[J].Northern Horticulture,2014,38(21):127-130.
“桃豫农矮砧1号”快繁体系中增殖培养基的初探
- Title:
- Selection of the Suitable Proliferation Medium in the Rapid Propagation System of ‘Peach Dwarf Yunong No.1 Rootstock’
- 文章编号:
- 1001-0009(2014)21-0127-04
- Keywords:
- peach; rapid propagation; proliferation medium; tissue culture
- 分类号:
- S 662.103.8
- 文献标志码:
- A
- 摘要:
- 以“桃豫农矮砧1号”的初代培养苗为外植体,以MS培养基为基本培养基,附加不同浓度的6-苄基腺嘌呤(6-BA)和 3-吲哚丁酸(IBA),筛选“桃豫农矮砧1号”快繁体系的最佳增殖培养基,从而为建立“桃豫农矮砧1号”高效的快繁体系提供参考依据。结果表明:MS+6-BA 1.5 mg/L+IBA 0.3 mg/L为最佳增殖培养基,在保证苗正常生长的同时,增殖倍数也最高,为2.33倍。
- Abstract:
- Taking original culture buds of ‘Peach dwarf Yunong No.1 rootstock’as explants,MS as basic medium,different concentration 6-BA and IBA were configured.The experiment was carried out by looking for the suitable proliferation medium of ‘Peach dwarf Yunong No.1 rootstock’ rapid propagation system,as a basis for establishing the ‘Peach dwarf Yunong No.1 rootstock’ rapid propagation system.The results showed that the best proliferation medium was MS+6-BA 1.5 mg/L+IBA 0.3 mg/L,which not only the culture seedling could be ensured,but also the highest proliferation multiples could be ensured,which was 2.33.
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备注/Memo
第一作者简介:杜保伟(1968-),男,硕士,副教授,现主要从事遗传育种与栽培等研究工作。E-mail:dubaowei626877@sina.com.
基金项目:河南省科技转化资助项目(30400224)。
收稿日期:2014-06-10