XU Qin,WANG Xiao-jun,WANG Hui,et al.Study on Isolation and Culture of the Mesophyll Protoplasts from Abutilon avicennae[J].Northern Horticulture,2013,37(07):170-172.
苘麻叶肉原生质体的分离和培养研究
- Title:
- Study on Isolation and Culture of the Mesophyll Protoplasts from Abutilon avicennae
- 文章编号:
- 1001-0009(2013)07-0170-03
- Keywords:
- Abutilon avicennae; protoplast; isolation
- 分类号:
- S 563.6
- 文献标志码:
- A
- 摘要:
- 以苘麻的叶片、种子为试材，对苘麻叶肉原生质体游离培养过程的影响因素进行了研究。结果表明：采用无菌苗幼叶作为原生质体分离起始材料效果较好；质壁分离4 h，纤维素酶0.5%+离析酶0.3%组合酶解12～14 h可以获得产量和活力较高的原生质体。在有机成分较丰富的DPD培养基中，细胞生长较为旺盛。
- Abstract:
- Taking the leaves and seeds of Abutilon avicennae as materials，the factors affecting the isolation mesophyll protoplasts from Abutilon avicennae were studied.The results showed that the best experiment materials were the leaves from the sterile seeding of Abutilon avicennae；if digested the leaves with combination of the enzyme solution 0.5% cellulase+0.3% macerozyme for 12~14 hours，more better protoplasts will be gotten.In the DPD liquid medium，the cells grew better.
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备注/Memo
第一作者简介：徐琴（1978-），女，硕士，助理研究员，现主要从事植物培养等研究工作。