[1]刘国丽,陈珣,马晓颖,等.平菇耐高温种质原生质体杂交育种及SSR遗传分析[J].北方园艺,2026,(9):9-16.[doi:10.11937/bfyy.20253720]
　LIU Guoli,CHEN Xun,MA Xiaoying,et al.Germplasm Protoplast Hybridization Breeding of High-temperature Tolerance Pleurotus ostreatus and SSR Genetic Analysis[J].Northern Horticulture,2026,(9):9-16.[doi:10.11937/bfyy.20253720]
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平菇耐高温种质原生质体杂交育种及SSR遗传分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年9 页码: 9-16 栏目: 出版日期: 2026-05-15
Title:
Germplasm Protoplast Hybridization Breeding of High-temperature Tolerance Pleurotus ostreatus and SSR Genetic Analysis
文章编号:
1001-0009(2026)09-0009-08
作者:
刘国丽; 陈珣; 马晓颖; 张鹏; 龚娜
（辽宁省农业科学院 食用菌研究所，辽宁 沈阳 110161）
Author(s):
LIU Guoli; CHEN Xun; MA Xiaoying; ZHANG Peng; GONG Na
(Institute of Edible Fungi，Liaoning Academy of Agricultural Sciences,Shenyang，Liaoning 110161)
关键词:
平菇; 原生质体单核体; 耐高温; SSR
Keywords:
Pleurotus ostreatus; protoplast monokaryon; high-temperature tolerance; simple sequence repeat
分类号:
S646.14
DOI:
10.11937/bfyy.20253720
文献标志码:
A
摘要:
以性状互补、耐高温能力差异显著的糙皮侧耳菌株GP20、GP26和GP27为试材，采用原生质体单核化方法及单杂交育种获得杂交菌株，采用显微镜观察锁状联合结构和拮抗试验初步鉴定杂交子，研究了杂交菌株的耐高温能力及SSR分子标记遗传多样性，以期为平菇耐高温菌株选育提供遗传材料和参考依据。结果表明：利用原生质体单核化技术获得GP20原生质体单核体菌株9份，原生质体单核化率为9.47%。交配型偏分离严重，均为A1B1交配型，通过将GP20原生质体单核体与GP26、GP27孢子单核体两两配对杂交后获得140组杂交组合，通过显微观察，初步获得35杂交子。经过培养传代，淘汰生长弱的菌株9份，将26个杂交菌株及3个亲本菌株进行耐高温能力评价，高温胁迫后菌丝生长速度显示杂交菌株LZ16、LZ18、LZ21、LZ40、LZ42、LZ45、LZ47、LZ62具有超双亲的优势，菌丝耐高温能力强。SSR分子标记聚类分析将26个菌株分为两大类群，在遗传距离0.84处可将亲本菌株与杂交菌株分开。
Abstract:
Taking Pleurotus ostreatus strains GP20，GP26 and GP27 with complementary agronomic traits and significantly divergent high temperature tolerance as test materials，hybrid strains were obtained via protoplast monokaryotization combined with mono-mono hybridization breeding.Hybrids were preliminarily identified through microscopic observation of clamp connection structures and antagonistic tests.Furthermore，the high-temperature tolerance and genetic diversity based on SSR molecular markers of the obtained hybrid strains were systematically investigated，in order to provide genetic resources and references for the breeding of high-temperature tolerance P.ostreatus strains.The results showed that 9 protoplast monokaryon strains of GP20 were obtained through protoplast monokaryotization，with a monokaryotization rate of 9.47%.Severe segregation distortion of mating types was observed，and all monokaryons belonged to the A1B1 mating type.A total of 140 hybrid combinations were obtained by pairwise hybridization between GP20 protoplast monokaryons and spore monokaryons of GP26 or GP27.Through microscopic observation，35 hybrid progeny were initially identified.After subculture，9 strains with weak growth were eliminated.The high-temperature tolerance of 26 hybrid strains and 3 parental strains was evaluated.Under high-temperature stress，the mycelial growth rate indicated that hybrid strains LZ16，LZ18，LZ21，LZ40，LZ42，LZ45，LZ47，and LZ62 exhibited transgressive heterosis，with strong mycelial high-temperature tolerance.SSR molecular marker clustering analysis divided the 26 strains into 2 major groups.Most hybrids were clustered with the parents，while some strains showed significant genetic variation and were not grouped with the parents.

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备注/Memo

第一作者简介：刘国丽（1986-），女，硕士，副研究员，现主要从事食用菌遗传育种等研究工作。E-mail：liugl12@163.com.责任作者：龚娜（1982-），女，硕士，副研究员，现主要从事食（药）用菌育种及栽培生理等研究工作。E-mai：doll52133@163.com.基金项目：辽宁省科技重大专项资助项目（2025JH1/11700018-02）；辽宁省科技特派行动专项计划资助项目（2025JH5/10400137,2025JH5/10400138）；辽宁省农业科学院院长基金资助项目（2024QN1804）。收稿日期：2025-10-27

更新日期/Last Update: 2026-05-19