JIA Yinghua,XIN Jianpan,ZHU Yuan,et al.Effects of Exogenous Melatonin on Seed Germination and Seedling Growth Characteristics of Miscanthus sacchariflorus Under Cd Stress[J].Northern Horticulture,2025,(12):74-82.[doi:10.11937/bfyy.20250200]
外源褪黑素对镉胁迫下荻种子萌发和幼苗生长特性的影响
- Title:
- Effects of Exogenous Melatonin on Seed Germination and Seedling Growth Characteristics of Miscanthus sacchariflorus Under Cd Stress
- 文章编号:
- 1001-0009(2025)12-0074-09
- Keywords:
- Miscanthus sacchariflorus (Maxim.) Benth.; melatonin; cadmium stress; seed germination; seedling growth
- 分类号:
- S 688.4;S 682.32
- 文献标志码:
- A
- 摘要:
- 以荻种子为试材，采用培养皿滤纸法，研究了不同浓度外源褪黑素(MT)(0、50、100 μmol·L-1)预处理对20 mg·L-1 镉(Cd)胁迫下荻种子萌发和幼苗生理特性的影响，以期为促进荻在Cd污染环境修复中的应用提供参考依据。结果表明：与CK相比，Cd胁迫的发芽势和发芽率分别降低了85.11%和74.27%。与Cd胁迫相比，Cd胁迫下50 μmol·L-1 MT处理的发芽势显著增加了85.71%，Cd胁迫下50、100 μmol·L-1 MT处理的发芽率显著提高了64.23%、19.51%。同时，Cd胁迫显著抑制荻种子发芽指数，并降低幼苗苗高、根长、生根数及鲜质量。外源MT预处理能够提高Cd胁迫下荻种子活力指数，并改善幼苗生长，表现为苗高、鲜质量的增加，但仍显著低于CK。与CK相比，Cd胁迫下可溶性糖含量显著提高了23.96%。与单独Cd胁迫相比，Cd胁迫下50、100 μmol·L-1 MT处理导致可溶性糖含量显著增加了25.28%、25.91%。与Cd胁迫下100 μmol·L-1 MT处理相比，Cd胁迫下50 μmol·L-1 MT预处理下荻种子发芽势、发芽率和活力指数均显著增加。因此，外源MT能够有效缓解20 mg·L-1Cd胁迫对荻种子萌发和幼苗生长的毒害作用，以50 μmol·L-1 MT为宜。
- Abstract:
- Taking the seeds of Miscanthus sacchariflorus (Maxim.) Benth.as the experimental material，the petri dish filter paper method was employed to investigate the effects of pretreatment with different concentrations of exogenous melatonin (melatonin,MT) (0，50 μmol·L-1，and 100 μmol·L-1) on seed germination and seedling physiological characteristics under 20 mg·L-1 cadmium (Cd) stress,in order to provide reference for enhancing the application of M.sacchariflorus in the remediation of Cd-contaminated environments.The results showed that compared to the control (CK)，Cd stress significantly reduced germination potential and germination rate by 85.11% and 74.27%，respectively.Compared with Cd stress alone，the germination potential of 50 μmol·L-1 MT treatment under Cd stress was significantly increased by 85.71%，and the germination rate of 50,100 μmol·L-1 MT treatments under Cd stress was significantly increased by 64.23% and 19.51%.Moreover，Cd stress markedly inhibited the germination index of M.sacchariflorus seeds and decreased plant height，root length，rooting number，and fresh weight.Exogenous MT pretreatment improved the seed vigor index under Cd stress and promoted seedling growth，as indicated by increased plant height and fresh weight，although these parameters remained significantly lower than those of CK.Compared to CK，the soluble sugar content under Cd stress increased significantly by 23.96%.Relative to Cd stress alone，the application of 50 μmol·L-1 and 100 μmol·L-1 MT treatment under Cd stress resulted in significant increases in soluble sugar content by 25.28% and 25.91%，respectively.Furthermore，compared to the 100 μmol·L-1 MT treatment under Cd stress，the 50 μmol·L-1 MT pretreatment under Cd stress significantly enhanced germination potential，germination rate，and vigor index.In conclusion，exogenous MT effectively mitigates the toxic effects of 20 mg·L-1 Cd stress on seed germination and seedling growth of M.sacchariflorus，with 50 μmol·L-1 MT treatment identified as the optimal concentration.
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备注/Memo
第一作者简介：贾英华(2000-)，女，硕士研究生，研究方向为园林植物生态修复。E-mail：2183364284@qq.com.责任作者：田如男(1969-)，女，博士，教授，现主要从事园林植物生理与生态修复等研究工作。E-mail：tianrunan@njfu.edu.cn.基金项目：国家自然科学基金资助项目(30972408)。收稿日期：2025-01-17