LU Mingmei,LIU Ping,SHI Ben,et al.Screening of Multifunctional Growth-promoting Bacteria in Tree Peony Rhizosphere and Analysis of Their Promoting Potential[J].Northern Horticulture,2024,(24):45-53.[doi:10.11937/bfyy.20241535]
牡丹根际多功能促生菌的筛选及其促生潜力分析
- Title:
- Screening of Multifunctional Growth-promoting Bacteria in Tree Peony Rhizosphere and Analysis of Their Promoting Potential
- 文章编号:
- 1001-0009(2024)24-0045-09
- Keywords:
- tree peony; growth-promoting rhizobacteria; growth promoting characteristics; microbial fertilizers
- 分类号:
- S 685.11
- 文献标志码:
- A
- 摘要:
- 以牡丹根际土壤为试材，采用筛选培养基对牡丹根际促生菌进行分离纯化，对筛选所得促生菌株的固氮、溶磷、分泌吲哚乙酸（IAA）、产铁载体、1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性等促生特性进行检测，同时利用盆栽试验分析筛选菌株对小白菜幼苗的促生效果，以期从牡丹根际筛选出具有多种促生功能的菌株，为开发高效微生物菌剂提供参考依据和优良菌种资源。结果表明：从牡丹根际土壤中筛选出8株多功能促生菌株，其中菌株N3、P2和N16兼具固氮、溶磷、产铁载体、分泌IAA能力，且菌株N3分泌IAA含量最高，为27.90 mg·L-1，P2溶解无机磷能力最强，溶磷圈直径/菌落直径（D/d）为3.77，其他菌株（L1、L5、L10、K5和N26）均具有2种及以上促生特性。菌株16S rDNA序列测定及系统发育分析结果表明菌株N3和P2属于泛菌属（Pantoea），菌株L1、L5和K5属于假单胞菌属（Pseudomonas），N16属于假黄色单胞菌属（Pseudoxanthomonas），L10和N26属于农杆菌属（Agrobacterium）。盆栽试验结果显示4株代表菌株对小白菜均有一定的促生效果，表明牡丹根际促生菌具有制备微生物肥料的潜力。
- Abstract:
- The rhizosphere soil of tree peony was used as the experimental material,the tree peony rhizosphere growth promoting bacteria were isolated and purified by the screening medium.The growth promoting characteristics of the screened strains,such as nitrogen fixation,phosphorus solubilization,indole-3-acetic acid (IAA) secretion,iron carrier production,and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity,were studied.At the same time,pot cultivation experiments were used to analyze the growth promoting effect of the screened strains on Chinese cabbage seedlings,in order to screen strains with multiple growth promoting functions from the tree peony rhizosphere and provide reference and excellent bacterial resources for the development of efficient microbial agents.The results showed that eight composite growth promoting strains were screened from the tree peony rhizosphere soil.Among them，N3，P2，and N16 showed the ability of nitrogen-fixing，phosphate-dissolving，siderophore and IAA production.The highest content of IAA secreted by N3 was 27.90 mg·L-1.P2 showed the strongest ability to dissolve inorganic phosphorus，and the ratio of the phosphorus solubilizing circle diameter and colony diameter (D/d) was 3.77.Other strains (L1，L5，L10，K5，and N26) all showed two or more growth promoting characteristics.The 16S rDNA sequencing and phylogenetic analysis of these strains indicated that N3 and P2 belonged to the genus Pantoea，L1，L5 and K5 belonged to the genus Pseudomonas，N16 belonged to the genus Pseudoxanthomonas，L10 and N26 belonged to the genus Agrobacterium.The results of the pot experiment showed that four representative strains had a certain promoting effect on the growth of Chinese cabbage，indicating that tree peony rhizosphere promoting bacteria had the potential to prepare microbial fertilizers.
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备注/Memo
第一作者简介：卢明媚（1988-），女，博士，讲师，现主要从事微生物资源开发利用等研究工作。E-mail:lumingmei163@163.com.责任作者：杨瑞先（1979-），女，博士，副教授，现主要从事植物病害及生物防治等研究工作。E-mail:fairy19790805@163.com.基金项目：洛阳理工学院高层次人才启动资助项目（2019BZ14）。收稿日期：2024-04-10