SHANG Wenqian,ZHANG Chunling,HE Songlin,et al.Effects of Different Light Qualities on Sucrose Metabolism and Related Enzyme Activities in vitro Plantlets of Paeonia suffruticosa[J].Northern Horticulture,2022,(13):79-86.[doi:10.11937/bfyy.20220358]
不同光质对牡丹试管苗糖代谢及相关酶活性的影响
- Title:
- Effects of Different Light Qualities on Sucrose Metabolism and Related Enzyme Activities in vitro Plantlets of Paeonia suffruticosa
- Keywords:
- Paeonia suffruticosa; light quality; sugar metabolism; in vitro
- 文献标志码:
- A
- 摘要:
- 以牡丹‘洛阳红’为试验材料，采用不同LED光源（红光(658 nm)、蓝光(461 nm)、绿光(520 nm)、黄光(596 nm)和普通荧光灯（CK））光照处理的方法，研究了不同光质对牡丹试管苗生长、糖代谢及相关酶活性的影响，以期为提高牡丹试管苗品质提供参考依据。结果表明：试管苗在红光下整体长势较好，在蓝光处理下鲜质量（总、茎、叶）和干质量（总、叶）最高，红光处理下茎干质量最高，黄光处理下的叶鲜质量和总鲜质量最低，CK处理下的鲜质量（茎）和干质量（总、茎、叶）最低，总鲜质量在绿光处理下高于红光处理，总干质量则与之相反。可溶性糖、蔗糖和果糖含量在红光处理下最高，葡萄糖在蓝光处理下最高；蔗糖合成酶（SS）和蔗糖磷酸合成酶（SPS）活性均在红光处理下最高，绿光处理和黄光处理下的SS和SPS活性均较低；酸性转化酶（AI）和中性转化酶（NI）均在蓝光处理下最高，红光处理下AI活性最低，黄光处理下NI活性最低。综上所述，蓝光有利于牡丹试管苗干鲜质量的提高；红光提高牡丹试管苗可溶性糖、蔗糖和果糖含量，蓝光提高其葡萄糖含量；黄光不利于试管苗的生长；绿光与红光干鲜质量的变化，可能与红光处理下高活性的SS活性和SPS活性、低活性的AI和NI有关，导致蔗糖含量升高，从而提高干质量相关。
- Abstract:
- Paeonia suffruticosa ‘Luoyanghong’ was used as experimental material，and different LED light sources （red light (658 nm)，blue light (461 nm)，green light (520 nm)，yellow light (596 nm) and common fluorescent lamp (CK)） were used to study the effects of different light qualities on the growth，sugar metabolism and related enzyme activities of Paeonia suffruticosa in vitro, in order to provide reference for improving the quality of Paeonia suffruticosa in vitro.The results showed that overall growth of test-tube seedlings were better under red light，the fresh weights (total，stem，leaf) and dry weights (total，leaf) were the highest under blue light treatment，the stem dry weight was the highest under red light treatment，and the leaf fresh weight and total fresh weight were the lowest in the yellow light treatment.The fresh weight (stem) and dry weights (total，stem and leaf) were the lowest under the CK treatment，the total fresh weight was higher in the green light treatment than in the red light treatment，and the total dry weight was the opposite.The contents of soluble sugar，sucrose and fructose were the highest under red light treatment，and glucose was the highest under blue light treatment.The activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) were both highest in the red light treatment，and the activities of SS and SPS were lower under green light treatment and yellow light treatment.The activities of acid invertase (AI) and neutral invertase (NI) were the highest under blue light treatment，AI activity was the lowest under red light treatment，and NI activity was the lowest under yellow light treatment.In conclusion，it showed that blue light treatment was beneficial to the dry and fresh weight of Paeonia suffruticosa in vitro；red light treatment increased the contents of soluble sugar，sucrose and fructose，and blue light treatment increased the glucose content；yellow light was unfavorable to the growth of test tube seedlings；the changes of dry and fresh weight of green light treatment and red light treatment were probably related to the high activity of SS and SPS and low activity of AI and NI under red light treatment，which led to the increase of sucrose content and thus dry weight.
参考文献/References:
[1]李岩,文莲莲,焦娟,等.不同转红光棚膜对甜椒光合特性及果实品质的影响[J].植物生理学报,2017,53(12)：2147-2156.[2]CHANG S,LI C,YAO X,et al.Morphological，photosynthetic，and physiological responses of rapeseed leaf to different combinations of red and blue lights at the rosette stage[J].Frontiers in Plant Science,2016,7(771):1144-1156.[3]张涛.不同LED光质和组配对叶用莴苣生理基础及营养品质的影响[D].郑州:河南农业大学,2018.[4]CHEN X L,YANG Q C.Effects of intermittent light exposure with red and blue light emitting diodes on growth and carbohydrate accumulation of lettuce[J].Scientia Horticulturae,2018,234:220-226.[5]CHOI H G,MOON B Y,KANG N J,Effects of LED light on the production of strawberry during cultivation in a plastic greenhouse and in a growth chamber[J].Scientia Horticulturae,2015,189:22-31.[6]陈晓丽,杨其长,张馨,等.LED绿光补光模式对生菜生长及品质的影响[J].中国农业科学,2017,50(21):4170-4177.[7]许大全,高伟,阮军.光质对植物生长发育的影响[J].植物生理学报,2015,51(8):1217-1234.[8]ROOK F,HADINGHAM S A,LI Y,et al.Sugar and ABA response pathways and the control of gene expression[J].Plant，Cell & Environment,2006,29(3):426-434.[9]耿艳秋,董肖昌,张春梅.园艺作物糖转运蛋白研究进展[J].园艺学报,2021,48(4):676-688.[10]靳晓白,成仿云,张启翔.国际栽培植物命名法规[M].北京:中国林业出版社,2013.[11]BERUTO M,LANTERI L,PORTOGALLO C.Micropropagation of tree peony (Paeonia suffruticosa)[J].Plant Cell，Tissue and Organ Culture,2004,79(2):249-255.[12]贺丹,王政,何松林.牡丹试管苗生根过程解剖结构观察及相关激素与酶变化的研究[J].园艺学报,2011,38(4):770-776.[13]张志良,瞿伟菁,李小方.植物生理学实验指导.4版[M].北京:高等教育出版社,2009.[14]江明艳,潘远智.不同光质对盆栽一品红光合特性及生长的影响[J].园艺学报,2006，33(2):338-343.[15]刘卫成,韦峰,韩泽宇,等.不同光质对黄瓜种子发芽、幼苗生长及抗寒性的影响[J].北方园艺,2016(19):59-62.[16]谢以萍,杨再强,苏天星,等.不同光质对瓜叶菊生长发育的影响[J].北方园艺,2010(3):53-56.[17]邵久之,韩杜斌,陈向荣,等.蓝光对黄瓜苗期形态和生理特性的影响[J].扬州大学学报(农业与生命科学版),2021,42(5):92-96,110.[18]王晓晶.LED绿光对蔬菜生长及品质的影响[D].太原:山西农业大学,2019.[19]刘敏玲.LED不同光质对金线莲生理特征及品质的影响[D].福州:福建农林大学,2013.[20]GUPTA S D,KUMAR A,AGARWAL A.Impact of light-emitting diodes (LEDs) on the growth and morphogenesis of encapsulated shoot buds of Curculigo orchioides Gaertn.an endangered medicinal herb[J].Acta Physiologiae Plantarum,2019,41(4):50.[21]张曼曼,赵晓红,魏宗法,等.LED不同光质对观赏海棠组培苗生长及生理特性的影响[J].山东农业科学,2018,50(10):43-47.[22]查凌雁,刘文科.不同光强下红蓝光配比对樱桃萝卜生长与产量的影响[J].中国农业气象,2018,39(3):162-167.[23]ZHU M,GENG S,CHAKRAVORTY D,et al.Metabolomics of red-light-induced stomatal opening in Arabidopsis thaliana:Coupling with abscisic acid and jasmonic acid metabolism[J].The Plant Journal,2020,101:1331-1348.[24]宋莹.不同光质对刺五加生理代谢影响[D].哈尔滨:东北林业大学,2021.[25]凌丹丹,雒佳铭,刘晓英,等.不同光质及组合对番茄开花初期碳氮代谢及其关键酶活性的影响[J].南京农业大学学报,2021,44(4):622-627.[26]孙娜,魏珉,李岩,等.光质对番茄幼苗碳氮代谢及相关酶活性的影响[J].园艺学报,2016,43(1):80-88.[27]HUANG Y W,NIE Y X,WAN Y Y,et al.Exogenous glucose regulates activities of antioxidant enzyme，soluble acid invertase and neutral invertase and alleviates dehydration stress of cucumber seedlings[J].Scientia Horticulturae,2013,162:20-30.[28]CHEN X L,WANG L C,LI T,et al.Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light[J].Scientific Reports,2019,9(1):6926.[29]孙娜.光质对番茄生长、生理代谢及果实产量品质的影响[D].泰安:山东农业大学,2015.
相似文献/References:
[1]郭丽丽,李 军,李金航,等.不同时期盆栽牡丹叶片RNA的提取研究[J].北方园艺,2014,38(12):99.
GUO Li-li,LI Jun,LI Jin-hang,et al.Extraction of Total RNA From Potted Tree Peony Leaves at Different Stages[J].Northern Horticulture,2014,38(13):99.
[2]刘 娜,秦安臣,陈 雪,等.牡丹对B9花期调控的响应研究[J].北方园艺,2014,38(10):56.
LIU Na,QIN An-chen,CHEN Xue,et al.Study on the Peony’s Flowering Regulation Response to B9[J].Northern Horticulture,2014,38(13):56.
[3]陈祥伟,刘世琦,刘 庆,等.不同LED光源对小白菜生长及光合特性的影响[J].北方园艺,2013,37(22):1.
CHEN Xiang-wei,LIU Shi-qi,LIU Qing,et al.Effects of Different Light Qualities on Growth and Photosynthetic Characteristics of Pakchoi[J].Northern Horticulture,2013,37(13):1.
[4]杨德翠,郑国生.柱枝孢叶斑病侵染对牡丹生理特性的影响[J].北方园艺,2014,38(01):57.
YANG De-cui,ZHENG Guo-sheng.Effects of Infection by Cylindrocladium canadenseon Physiological Characteristics of Tree Peony[J].Northern Horticulture,2014,38(13):57.
[5]高宏秀,张莹.牡丹春节催花技术[J].北方园艺,2014,38(06):72.
GAO Hong-xiu,ZHANG Ying.Flower Forcing Techniques of Tree Peony in Winter[J].Northern Horticulture,2014,38(13):72.
[6]郑玲,程彦伟.六个洛阳牡丹品种解剖结构与抗旱性关系研究[J].北方园艺,2013,37(01):65.
ZHENG Ling,CHENG Yan-wei.Study on Relationship Between Anatomical Structures and Drought Resistance of Some Cultivars of Luoyang Paeonia suffruticosa Andr.[J].Northern Horticulture,2013,37(13):65.
[7]史倩倩,王雁,周琳,等.生根粉及不同基质对牡丹传统品种扦插的影响[J].北方园艺,2012,36(01):91.
SHI Qian-qian,WANG Yan,ZHOU Lin,et al.Influence of Rooting Powder and Culture Substrata on Rooting of Different Traditional Cultivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(13):91.
[8]孙文泰,尹晓宁,刘兴禄,等.不同海拔高度对“红富士”苹果果实品质的影响[J].北方园艺,2013,37(06):12.
SUN Wen-tai,YIN Xiao-ning,LIU Xing-lu,et al.Effect of Different Altitudes on Quality of ‘Red Fuji’ Apple[J].Northern Horticulture,2013,37(13):12.
[9]王 玮,张旭东,施 江,等.不同品种牡丹萌芽过程中组织抗冷性的变化[J].北方园艺,2013,37(06):52.
WANG Wei,ZHANG Xu-dong,SHI Jiang,et al.Changes of Cold Resistance of Floral Organs During Sprout in Different Tree Peony Cultivars[J].Northern Horticulture,2013,37(13):52.
[10]刘帅,丰震,徐艳,等.牡丹不同品种花期差异的生理机理研究[J].北方园艺,2012,36(03):67.
LIU Shuai,FENG Zhen,XU Yan,et al.Study on the Physiological Mechanism of Flowering Phase Difference in Cutivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(13):67.
备注/Memo
第一作者简介：尚文倩（1987-），女，博士，讲师，现主要从事园林植物栽培生理与生物技术等研究工作。E-mail：wenqianshang@henau.edu.cn.责任作者：何松林（1965-），男，博士，教授，现主要从事园林植物栽培生理与生物技术等研究工作。E-mail：hsl213@163.com.基金项目：上海市资源植物功能基因组学重点实验室开放课题资助项目（PFGR202102）；国家自然科学基金资助项目（31870698，32001353）；河南省重点研发与推广专项资助项目（202102110083）。收稿日期：2022-01-26