[1]叶嘉,李建飞,陈江魁,等.涉县柴胡茎叶总黄酮提取优化及其抗氧化性[J].北方园艺,2022,(06):92-98.[doi:10.11937/bfyy.20214464]
　YE Jia,LI Jianfei,CHEN Jiangkui,et al.Optimization of Extraction Technology of Total Flavonoids and Its Antioxidant Capacity From Aerial Part of Shexian Bupleurum[J].Northern Horticulture,2022,(06):92-98.[doi:10.11937/bfyy.20214464]
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涉县柴胡茎叶总黄酮提取优化及其抗氧化性

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年06 页码: 92-98 栏目: 出版日期: 2022-03-30
Title:
Optimization of Extraction Technology of Total Flavonoids and Its Antioxidant Capacity From Aerial Part of Shexian Bupleurum
作者:
叶嘉1; 2; 李建飞3; 陈江魁1; 2; 施明丽1; 杨香瑜1; 2; 郭海燕1; 2
（1.邯郸学院 生命科学与工程学院，河北 邯郸 056005；2.河北省高校冀南太行山区野生资源植物应用研发中心，河北 邯郸 056005；3.涉县擎阳种业有限公司，河北 邯郸 056400）
Author(s):
YE Jia1; 2; LI Jianfei3; CHEN Jiangkui1; 2; SHI Mingli1; YANG Xiangyu1; 2; GUO Haiyan1; 2
(1.College of Life Science and Engineering，Handan University，Handan，Hebei 056005；2.Wild Plant Resources Research Centre in Taihang Mountain of Southern Hebei Province，Handan，Hebei 056005；3.Shexian Qingyang Seed Industry Co.Ltd.，Handan,Hebei 056400)
关键词:
柴胡茎叶; 总黄酮; 优化; 抗氧化活性
Keywords:
aerial part of bupleurum; total flavonoids; optimization; antioxidant activity
DOI:
10.11937/bfyy.20214464
文献标志码:
A
摘要:
以涉县柴胡茎叶为试材，采用单因素试验和四因素三水平的正交实验设计，研究优化了总黄酮乙醇回流浸提工艺，并分析测定提取物及与维生素C复配后的抗氧化活性，以期为涉县柴胡的进一步开发利用提供参考依据。结果表明：1）涉县柴胡茎叶总黄酮最佳提取工艺为料液比1∶20 g?mL-1，提取时间2 h，提取温度70 ℃，乙醇浓度60%；2）最优条件下涉县柴胡茎叶总黄酮对DPPH?、?OH、O?2的半抑制浓度分别为0.223、0.413 mg?mL-1和0.445 mg?mL-1；〖JP+1〗3）与维生素C复配后表现出一定协同作用，试验组内复配液（1∶3）协同作用最强。综上可知，涉县柴胡茎叶中总黄酮具有很好的抗氧化能力。
Abstract:
Taking the aerial part of Shexian bupleurum as test materials,the ethanol reflux extraction process of total flavonoids was studied and optimized by single factors test and four factors and three-levels orthogonal test.The antioxidant activity of the extract and compound with vitamin C were studied,in order to provide reference for the further development and utilization of bupleurum in Shexian.The results showed that,1) the best extraction process for total flavonoids from aerial part of bupleurum was solid-liquid ratio was 1∶20 g?mL-1，extraction time was 2 hours，extraction temperature was 70 ℃，and ethanol concentration was 60%.2) The half inhibition rate concentration (IC50) of total flavonoids from aerial part of bupleurum against DPPH?，?OH and O?2 radical were 0.223，0.413 mg?mL-1 and 0.445 mg?mL-1，respectively.3) It showed a certain synergistic effect after compounding with vitamin C，the best synergistic effect was compound solution (1∶3) in all experimental groups.In conclusion,the total flavonoids in the aerial part of Shexian bupleurum had good antioxidant capacity.

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备注/Memo

第一作者简介：叶嘉（1963-），女，硕士，教授，现主要从事资源植物开发利用等研究工作。E-mail:cjk02006@126.com.基金项目：河北省创新能力提升计划资助项目（20536404D）。收稿日期：2021-11-04

更新日期/Last Update: 2022-05-25