[1]苏秀丽,李惠敏,唐辉,等.黄花倒水莲内生真菌多样性及抑菌活性分析[J].北方园艺,2022,(06):99-106.[doi:10.11937/bfyy.20213732]
　SU Xiuli,LI Huimin,TANG Hui,et al.Diversity and Antifungal Activities of Endophytic Fungi in Polygala fallax Hemsl[J].Northern Horticulture,2022,(06):99-106.[doi:10.11937/bfyy.20213732]
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黄花倒水莲内生真菌多样性及抑菌活性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年06 页码: 99-106 栏目: 出版日期: 2022-03-30
Title:
Diversity and Antifungal Activities of Endophytic Fungi in Polygala fallax Hemsl
作者:
苏秀丽1; 2; 李惠敏1; 唐辉2; 张玉仙2; 3; 刘宝玉2; 3
（1.广西师范大学 珍稀濒危动植物生态与环境保护教育部重点实验室，广西 桂林 541006；2.广西壮族自治区中国科学院广西植物研究所，广西 桂林 541006；3.广西植物功能物质研究与利用重点实验室，广西 桂林 541006）
Author(s):
SU Xiuli1; 2; LI Huimin1; TANG Hui2; ZHANG Yuxian2; 3; LIU Baoyu2; 3
(1.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection，Guangxi Normal University，Guilin，Guangxi 541006；2.Guangxi Institute of Botany，Chinese Academy of Sciences，Guangxi Zhuang Autonomous Region，Guilin，Guangxi 541006；3.Key Laboratory of Plant Functional Substance Research，Guangxi Institute of Botany，Guilin，Guangxi 541006)
关键词:
黄花倒水莲; 内生真菌; 多样性; 抑菌活性
Keywords:
Polygala fallax Hemsl; endophytic fungi; diversity; antifungal activity
DOI:
10.11937/bfyy.20213732
文献标志码:
A
摘要:
以桂林市灵川县的黄花倒水莲为试材，采用组织块分离法、ITS测序和平板对峙等方法，研究了黄花倒水莲内生真菌的菌群组成及抗菌活性，以期为开发生防菌提供参考依据。结果表明：从黄花倒水莲中共分离得到62株内生真菌，经分子鉴定属于29个属，优势属为刺盘孢属(Colletotrichum sp.)、间座壳属(Diaporthe sp.)、镰孢菌属(Fusarium sp.)和拟茎点霉属(Phomopsis)。筛选到10株对香蕉黑条叶斑病病菌、柑橘树脂病病菌、叶点霉病菌具有较强抑菌活性的菌株。药用植物黄花倒水莲具有较为丰富的抑菌活性内生真菌资源，其抑菌活性值得深入研究。
Abstract:
Polygala fallax Hemsl collected from Lingchuan in Guilin was used as the test material.The tissue block separation,ITS sequencing and plate sputum method were performed to study the composition and antifungi activity of endophytic fungi of Polygala fallax Hemsl,in order to provide reference for the development of biocontrol fungi.The results showed that a total of 62 isolates of endophytic fungi were obtained.They were classified into 29 different genus according to their ITS characters,and Colletotrichum sp.,Diaporthe sp.,Fusarium sp.,Phomopsis sp.were the dominant genus.Ten isolates showed antifungi activity against Mycosphaerella fijiensis,Diaporthe citri (Fawcett) wolf,Phyllosticta zingiberi.The medicinal plant Polygala fallax Hemsl showed relatively abundant endophytic fungal resource for pathogenic inhibition,and its antifungi inhibition was worthwhile for further study.

参考文献/References:

[1]ZU J D，REN D Z，YANG S，et al.Characterization of Cd-，Pb-，Zn-resistant endophytic Lasiodiplodia sp.MXSF31 from metal accumulating Portulaca oleracea and its potential in promoting the growth of rape in metal-contaminated soils[J].Environmental Science and Pollution Research，2014,21(3)：2346-2357.[2]邓文祥，赵漫丽，李永梅，等.白及根部内生真菌多样性研究[J].菌物学报，2019,38(11):1907-1917.[3]颜桢灵，李国萍，骆海玉，等.血散薯内生真菌的分离鉴定及其抗菌活性研究[J].河南农业科学，2019,48(10):84-92.[4]赖清玉，赵明富，保颖，等.雪胆内生真菌的分离与鉴定研究[J].中药材，2019,42(8):1771-1776.[5]张誉荠，文湘穗，王雨濛，等.北五味子种子内生真菌的分离鉴定及抑菌性研究[J].中药材，2020,43(5):1087-1091.[6]李亮，张占英，孙慧英，等.农药危害与绿色植保技术探讨[J].湖北植保，2018(4):63-64.[7]邱德文.生物农药的发展现状与趋势分析[J].中国生物防治学报，2015,31(5):679-684.[8]KAUL S，GUPTA S，AHMED M，et al.Endophytic fungi from medicinal plants：A treasure hunt for bioactive metabolites[J].Phytochemistry Reviews,2012，11(4)：487-505.[9]KUSARI S，PANDEY S P，SPITELLER M.Untapped mutualistic paradigms linking host plant and endophytic fungal production of similar bioactive secondary metabolites[J].Phytochemistry，2013，91：81-87.[10]姜道宏.植物内生真菌及其展望[J].中国生物防治学报，2015,31(5):742-749.[11]SUSHEEL K，NUTAN K.Metabolites of endophytic fungi as novel source of biofungicide：A review[J].Phytochemistry Reviews，2012,11(4)：507-522.[12]王子威，何中声，刘金福.黄花倒水莲栽培及利用研究综述[J].中国野生植物资源，2016,35(4):48-52.[13]曹后康，韦日明，张可锋，等.黄花倒水莲多糖对四氯化碳致急性肝损伤小鼠的保护作用[J].中药材，2018,41(1):203-206.[14]张嫦丽，张可锋，许有瑞，等.黄花倒水莲的化学成分与药理活性研究进展[J].中国药房，2017,28(19):2724-2728.[15]姚志仁，李豫，朱开梅，等.黄花倒水莲不同极性部位抗氧化和降血糖活性研究[J].食品工业科技，2020,41(7):55-59.[16]YAO Y Q，LAN F，QIAO Y M，et al.Endophytic fungi harbored in the root of Sophora tonkinensis Gapnep：Diversity and biocontrol potential against phytopathogens[J].Microbiology Open，2017,6(3):e00437.[17]HAN D，WANG L，LUO Y.Isolation，identification，and the growth promoting effects of two antagonistic actinomycete strains from the rhizosphere of Mikania micrantha Kunth.[J].Microbiological Research，2018,208:1-11.[18]施文广，梁金虎，冯美茹，等.檀香紫檀内生真菌多样性与抗菌活性分析[J].南方农业学报，2021,52(2):538-546.[19]胡永志，杨鑫凤，周雅琴，等.两面针内生真菌遗传多样性分析及其抗菌活性研究[J].中国中药杂志，2021,46(13):3349-3355.[20]王嫚，吴智楠，周天赐，等.胡蔓藤内生真菌分离鉴定及抗菌活性研究[J].生物学通报，2021,56(2):43-47.[21]谭小明，周雅琴，陈娟，等.药用植物内生真菌多样性研究进展[J].中国药学杂志，2015,50(18):1563-1580.[22]MORGANE E，SANDRINE E，FABIENNE E，et al.Spatial and temporal variation of cultivable communities of co-occurring endophytes and pathogens in wheat[J].Frontiers in Microbiology，2016(7):403.[23]崔文霞，陈睿，张峰华，等.鱼腥草内生真菌多样性分析[J].西北农业学报，2017,26(10):1513-1519.[24]STPHANE C，KARI S，BIRGIT M，et al.Editorial special issue：Soil，plants and endophytes[J].Plant and Soil，2016,405(1):1-11.[25]PHILIPP H F，ALGA Z.Dissecting endophytic lifestyle along the parasitism/mutualism continuum in Arabidopsis[J].Current Opinion in Microbiology，2016(32):103-112.[26]XIAO-YU S，HUIHUA W，FEI R，et al.An endophytic diaporthe apiculatum produces monoterpenes with inhibitory activity against phytopathogenic fungi[J].Antibiotics，2019,8(4):231.[27]DONG H Y，XIAO Y S，HONG C L，et al.Antifungal activities of volatile secondary metabolites of four diaporthe strains isolated from Catharanthus roseus[J].Journal of Fungi，2018,4(2):65.[28]CAROLINE M D R，BRBARA V D R，GISELE P D R，et al.Antifungal and antibacterial activity of extracts produced from Diaporthe schini[J].Journal of Biotechnology，2019,294:30-37.[29]李丰名，陈嘉颖，吕镇城，等.酸模内生镰刀菌HU0298抗MRSA活性代谢成分[J].菌物学报，2021,40(5):1140-1147.[30]丁唯嘉，叶浚韬，佘志刚，等.一株红树林真菌Fusarium sp.R5固相发酵代谢产物[J].中山大学学报(自然科学版)，2021,60(5):136-140.[31]胡征波，吴友根，易天凤，等.50株诺丽内生真菌抑菌活性的比较及其菌株鉴定[J].热带生物学报，2018,9(4):433-439.[32]XIAO J，ZHANG Q，GAO Y，et al.Secondary metabolites from the endophytic Botryosphaeria dothidea of Melia azedarach and their antifungal，antibacterial，antioxidant，and cytotoxic activities[J].Journal of Agricultural and Food Chemistry，2014,62(16):3584.[33]毕江涛，潘润霞，李乐，等.牛心朴子内生真菌分离及其抑菌活性分析[J].草地学报，2014,22(1):188-193.[34]樊苗苗，陈肖学，张訸，等.樟叶越桔叶内生真菌的抑菌活性研究[J].西南林业大学学报(自然科学版)，2018,38(2):141-147.[35]陈肖学，樊苗苗，张訸，等.樟叶越桔1株内生真菌的鉴定及其抑菌活性[J].西南林业大学学报(自然科学版)，2018,38(4):187-192.

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备注/Memo

第一作者简介：苏秀丽（1996-），女，硕士研究生，研究方向为微生物学。E-mail:1808891670@qq.com.责任作者：唐辉（1972-），男，本科，研究员，现主要从事特色经济植物资源保护及可持续利用等研究工作。E-mail：913529761@qq.com.基金项目：广西科技重大专项资助项目（桂科AA18118015）；桂林市重大专项资助项目 (20190101)；桂林科技攻关资助项目(20190208-3)；广西植物功能物质研究与利用重点实验室资助项目（ZRJJ2018-7，ZRJJ2020-5）。收稿日期：2021-09-08

更新日期/Last Update: 2022-05-25