LI Xueyan,HU Xinying,WANG Weidong,et al.Establishment of in vitro Direct Regeneration System on Virus-free Bulblets of ‘Sorbonne’[J].Northern Horticulture,2021,(09):80-86.[doi:10.11937/bfyy.20201935]
无病毒百合‘Sorbonne’试管内小鳞茎直接再生体系建立
- Title:
- Establishment of in vitro Direct Regeneration System on Virus-free Bulblets of ‘Sorbonne’
- Keywords:
- Lilium; virus-free; bulblets in vitro; direct regeneration; breeder′s stock
- 文献标志码:
- A
- 摘要:
- 以‘Sorbonne’种球为试材，采用RT-PCR技术检测获得无CMV、LSV、LMoV的‘Sorbonne’种球，剥取其中外层鳞片通过组培获得无菌试管苗，以无菌苗鳞片为次级外植体，研究了不同植物生长调节剂对试管内小鳞茎直接再生的影响，以期为繁育优质的无病毒百合种球提供参考依据。结果表明，试管内小鳞茎直接再生的最适培养基为MS+2,4-D 1.0 mg?L-1，诱导率与诱导系数为100%、3.69；小鳞茎膨大最适培养基为MS+蔗糖90 g?L-1；生根最适培养基为MS+NAA 0.1 mg?L-1，移栽成活率可达100%。
- Abstract:
- Taking ‘Sorbonne’ bulbs as test material,the ‘Sorbonne’ bulbs without CMV，LSV and LMoV virus were obtained by RT-PCR.Scales excised of in vitro stock were used as secondary explants to explore the effects of different plant growth regulators on the direct regeneration of bulblets in vitro,in order to provide reference for breading high-quality virusfree lily bulbs.The results showed that the optimal medium for direct regeneration of bulblets in vitro was MS+2，4-D 1.0 mg?L-1，and the induction rate and induction coefficient were 100% and 3.69，respectively.The optimal medium for small bulb enlargement was MS+sucrose 90 g?L-1，MS+NAA 0.1 mg?L-1 was best for rooting，and the regenerated plantlets survived of ‘Sorbonne’ with highest rate of 100.00%.
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备注/Memo
第一作者简介：李雪艳（1986-），女，博士，助理研究员，现主要从事百合栽培与生物技术等研究工作。E-mail：lixueyan321@163.com.责任作者：杨迎东（1973-），男，硕士，研究员，现主要从事花卉栽培、育种及种球繁育技术等研究工作。E-mail：yangyingdong2011@163.com.基金项目：辽宁省自然科学基金资助项目（20170540503，20170520065，2019-MS-193，2019-ZD-0379）。收稿日期：2020-05-12