HU Shan,LIANG Weiqu,HUANG Hao,et al.Screening and Identification of Potassium Solubilizing Bacteria in Soil Composted with Chinese Medicine Residue[J].Northern Horticulture,2021,(09):87-92.[doi:10.11937/bfyy.20203346]
中药渣堆肥土壤中解钾细菌的筛选和鉴定
- Title:
- Screening and Identification of Potassium Solubilizing Bacteria in Soil Composted with Chinese Medicine Residue
- 文献标志码:
- A
- 摘要:
- 以堆肥2个月后的带土中药渣为试材，采用稀释平板法分离解钾细菌，并进行活性评估及鉴定,以期为制备高效生物有机肥储备苗株提供参考依据。结果表明：共分离6株解钾细菌（DGNK-JJ1、DGNK-JJ2、DGNK-JJ3、DGNK-JJ4、DGNK-JJ5、DGNK-JJ6），在30 ℃，150 r?min-1条件下发酵培养5 d后，DGNK-JJ1菌株的发酵液中速效钾含量最高，达到1.84 mg?L-1，比对照增加了93.72%。结合菌落形态特征、生理生化特征和16S rDNA序列分析结果，确定菌株DGNK-JJ1为纤维化纤维微菌（Cellulosimicrobium cellulans）。采用K-B纸片扩散法对菌株DGNK-JJ1进行药敏试验，其对氯霉素、多西环素、万古霉素、恩诺沙星、利福平、庆大霉素6种抗生素高度敏感。菌株DGNK-JJ1可作为开发生物钾肥的储备菌株。
- Abstract:
- Chinese medicine residue with soil after two months composting was used as test material.The isolation,activity evaluation and identification of postassium bacteria were studied by using dilution plate method,in order to provide a reference for the precparation of efficient bioorganic fertilizer reserve strains.The results showed that six strains (DGNK-JJ1,DGNK-JJ2,DGNK-JJ3,DGNK-JJ4,DGNK-JJ5 and DGNK-JJ6） were isolated.After five days fermentation under the condition of 30 ℃,150 r?min-1,the content of available potassium in fermentation liquid of DGNK-JJ1 reached a maximum of 1.84 mg?L-1.It was 93.72% higher than the blank control.Together with colony morphology,physiological and biochemical characteristics,16S rDNA sequence analysis revealed DGNK-JJ1 as Cellulosimicrobium cellulans.K-B disk diffusion method was used to test the drug sensitivity of DGNK-JJ1.The strain was highly sensitive to chloramphenicol,doxycycline,vancomycin,enrofloxacin,rifampicin,gentamicin.Strain DGNK-JJ1 can be used as a reserve strain for the development of biological potassium fertilizer.
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备注/Memo
第一作者简介：胡珊（1981-），女，硕士，高级农艺师，现主要从事农业生物技术等研究工作。E-mail:sh8199@126.com.责任作者：梁卫驱（1980-），男，硕士，高级农艺师，现主要从事农业生物技术等研究工作。E-mail:lwqkml@126.com.基金项目：广东省科技计划资助项目（2017A020208002）；东莞市科技转化与推广服务资助项目（2020B03025815）。收稿日期：2020-08-07