HUANG Yanyan,ZHANG Jin,FU Junhui,et al.Artificial Propagation Technology of Dendrobium officinale[J].Northern Horticulture,2021,(02):115-123.[doi:10.11937/bfyy.20200459]
铁皮石斛人工繁育技术
- Title:
- Artificial Propagation Technology of Dendrobium officinale
- 文献标志码:
- A
- 摘要:
- 以铁皮石斛商品瓶苗和盆栽苗茎段为试材，添加不同浓度6-BA、NAA，诱导茎段腋芽、原球茎和不定根，建立再生体系，快速繁殖铁皮石斛组培苗，以期为秦岭淮河一线以北地区人工繁殖铁皮石斛提供参考依据。结果表明：外植体宜采用70%酒精浸泡20 s，0.1%升汞浸泡8 min消毒；MS+6-BA 3.0~5.0 mg?L-1+NAA 0.1~1.0 mg?L-1适合腋芽分化和增殖，分化率92%~100%；1/2MS+6-BA 2.0 mg?L-1+NAA 0.5 mg?L-1+土豆泥15%适合原球茎诱导增殖，增殖倍数7.7；1/2MS+NAA 0.3 mg?L-1生根率为100%；以松针土移栽，成活率83%。
- Abstract:
- In order to provide technical reference for artificial breeding of Dendrobium officinale situated areas north,taking purchased tissue culture seedlings and potted seedlings as test materials,different concentrations of 6-BA and NAA were used to induce axillary buds，protocorms and adventitious roots to establish regeneration system,rapid propagation tissue culture seedlings of Dendrobium officinale.The results showed that the explants should be disfected by 70% alcohol for 20 seconds and 0.1% mercuric chloride for 8 minutes.MS+6-BA 3.0-5.0 mg?L-1+NAA 0.1-1.0 mg?L-1 was suitable for axillary buds differentiation and proliferation.The differentiation rate was 92%-100%；1/2MS medium with 6-BA 2.0 mg?L-1+NAA 0.5 mg?L-1+ mashed potato 15% was suitable for protocorms proliferation with a proliferation multiple of 7.7.In 1/2MS medium with NAA 0.3 mg?L-1,the rooting rate was 100%.Transplanting with pine needle soil，the survival rate was 83%.
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备注/Memo
第一作者简介：黄艳艳（1978-），女，硕士，高级工程师，现主要从事药用植物生物育种等研究工作。E-mail：yanhuang0321@163.com.责任作者：仉劲（1989-），男，博士，农艺师，现主要从事药用植物资源等研究工作。E-mail：zhjin0214@163.com.基金项目：山东省农业良种工程资助项目(2017LZN039)；2018年中医药公共卫生服务补助专项资助项目(财社[2018]43号)。收稿日期：2020-02-10