PEI Hua-li,LI Mei-qin,LIU Yong-guang,et al.Study on Tissue Culture and Regenerative System of Differernt Varieties of Tomato and Differernt Explants[J].Northern Horticulture,2013,37(03):119-121.
不同基因型番茄高效组培再生体系的建立
- Title:
- Study on Tissue Culture and Regenerative System of Differernt Varieties of Tomato and Differernt Explants
- 文章编号:
- 1001-0009(2013)03-0119-03
- Keywords:
- tomato; tissue culture; regeneration system
- 分类号:
- S 641.203.6
- 文献标志码:
- A
- 摘要:
- 以‘特大瑞光’、‘菜都982F1’和‘菜都六号’3种番茄为试材，用番茄的下胚轴、子叶、真叶为外植体，研究了不同基因型、不同外植体材料和不同激素浓度对番茄再生体系的影响，以期筛选出适宜不同基因型番茄离体培养的最佳培养条件。结果表明：3个番茄品种均在MS+2.0 mg/L BA+0.30 mg/L NAA培养基中的愈伤组织诱导率最高。‘特大瑞光’诱导不定芽分化的最佳培养基为MS+2.0 mg/L BA+0.10 mg/L NAA；“菜都982F1”诱导不定芽分化的最佳培养基为MS+2.0 mg/L BA+0.05 mg/L NAA；“菜都六号”诱导不定芽分化的最佳培养基为MS+3.0 mg/L BA+0.05 mg/L NAA。3个不同番茄品种在MS+0.05 mg/L NAA培养基中均获得了最佳的生根效果。
- Abstract:
- Taking ‘Tedaruiguang’，‘Caidu982F1’and ‘Caidu No.6’ as materials，the hypocotyls，with contyledon and true leaf of tomato as explants，the effect of different genotype,different explants and different concentrations of hormone on regenerative system of tomato were studied，in order to obtain the best way of regenerative system of different varieties of tomato.The results showed that callus of three different varietal tomatoes could be induced in MS media with 2.0 mg/L BA plus 0.30 mg/L NAA.The suitable differentiation medium for‘Tedaruiguang’ was MS+2.0 mg/L BA+0.10 mg/L NAA.The suitable differentiation medium for ‘Caidu982F1’was MS+2.0 mg/L BA+0.05 mg/L NAA.The suitable differentiation medium for ‘Caidu No.6’was MS+3.0 mg/L BA+0.05 mg/L NAA.The suitable rooting medium for three different varietal tomatoes was MS+0.05 mg/L NAA.
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[J].Northern Horticulture,2002,26(03):0.[doi:10.11937/bfyy.200205050]
备注/Memo
第一作者简介：裴华丽（1978-），女，山东潍坊人，硕士，讲师，现主要从事植物生物技术等研究工作。E-mail:peihuali2@163.com.