[1]朱雪妹,魏小红,金宝霞,等.番茄材料‘851’高效离体再生体系的建立[J].北方园艺,2020,44(13):1-8.[doi:10.11937/bfyy.20194441]
　ZHU Xuemei,WEI Xiaohong,JIN Baoxia,et al.Establishment of High Efficient in vitro Regeneration System of Material ‘851’ of Tomato[J].Northern Horticulture,2020,44(13):1-8.[doi:10.11937/bfyy.20194441]
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番茄材料‘851’高效离体再生体系的建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年13 页码: 1-8 栏目: 出版日期: 2020-07-15
Title:
Establishment of High Efficient in vitro Regeneration System of Material ‘851’ of Tomato
作者:
朱雪妹; 魏小红; 金宝霞; 张小芳; 刘放
（甘肃农业大学 生命科学技术学院，甘肃省作物遗传改良与种质创新重点实验室,甘肃省干旱生境作物学重点实验室，甘肃 兰州 730070）
Author(s):
ZHU Xuemei; WEI Xiaohong; JIN Baoxia; ZHANG Xiaofang; LIU Fang
(College of Life Science and Technology，Gansu Agricultural University/Gansu Key Lab of Crop Genetic & Germplasm Enhancement/Gansu Provincial Key Lab of Aridland Crop Science,Lanzhou，Gansu 730070)
关键词:
番茄; 外植体; IAA浓度筛选; 离体再生
Keywords:
tomato; explant; concentration screening of IAA; regeneration in vitro system
DOI:
10.11937/bfyy.20194441
文献标志码:
A
摘要:
以自育番茄‘851’为试材，采用植物组织培养的方法，研究了不同浸泡时间和光暗培养处理对番茄种子萌发和幼苗生长状态的影响；以MS+2.0 mg?L-1 6-BA为基础培养基研究不同IAA浓度、苗龄和外植体对愈伤和不定芽形成的影响；用不同处理的生根培养基研究不定芽对生根的影响，以期建立番茄材料‘851’高效离体再生体系。结果表明：番茄种子浸泡3 h，先暗培养3 d后光培养能够获得最佳生长状态的无菌苗且其发芽势、发芽率和发芽指数均为最高，分别为89%、93%和5.20。子叶节、下胚轴和子叶为外植体在IAA浓度为0.2、0.3、0.5 mg?L-1时最有利于愈伤组织和不定芽的形成，同时在10、14、8 d的苗龄下，不定芽诱导率分别为160%、91%和79%。最佳处理生根培养基为MS+0.5 mg?L-1 IAA，生根率为89%。
Abstract:
The self-cultivated tomato ‘851’ was used as the test material，and the method of plant tissue culture was used to study the effects of different soaking time,light and dark culture treatment on tomato seed germination and seedling growth status；6-BA was used as the basic medium to study the effects of different IAA concentrations，seedling ages and explants on callus and adventitious bud formation；the rooting medium with different treatments was used to study the effect of adventitious buds on rooting with a view to establishing tomato material ‘851’ efficient in vitro regeneration system.The results showed that the tomato seeds were soaked for 3 hours，and then cultured in the dark for 3 days and then light cultured to obtain the sterile seedlings with the best growth state，and their,germination potential，germination rate and germination index were the highest，89%，93% and 5.20 respectively.Cotyledonary nodes，hypocotyls and cotyledons were explants.IAA concentrations of 0.2，0.3，0.5 mg?L-1 were most beneficial to the formation of callus and adventitious buds，and at the age of 10，14，8 days the induction rate of adventitious buds was 160%，91% and 79%，respectively.The optimal rooting medium for treatment was MS+0.5 mg?L-1 IAA with a rooting rate of 89%.

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备注/Memo

第一作者简介：朱雪妹（1994-），女，福建三明人，硕士研究生，研究方向为利用amiRNA技术培育番茄抗TYLCV。E-mail:2928143741@qq.com.责任作者：魏小红（1964-），女，甘肃天水人，博士，教授，博士生导师，现主要从事植物逆境生理与分子生理等研究工作。E-mail:weixh@gsau.edu.cn.基金项目：国家自然科学基金资助项目（31560663）。收稿日期：2020-03-09

更新日期/Last Update: 2020-09-02