[1]高升华,李宁,王飞,等.辣椒抗疮痂病基因Bs2和Bs3[J].北方园艺,2020,44(13):9-15.[doi:10.11937/bfyy.20194298]
　Establishment and Application of Duplex PCR to Detection of Bacterial Spot Disease Resistance Genes Bs and Bs in Pepper.Establishment and Application of Duplex PCR to Detection of Bacterial Spot Disease Resistance Genes Bs2 and Bs3 in Pepper[J].Northern Horticulture,2020,44(13):9-15.[doi:10.11937/bfyy.20194298]
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辣椒抗疮痂病基因Bs2和Bs3

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年13 页码: 9-15 栏目: 出版日期: 2020-07-15
Title:
Establishment and Application of Duplex PCR to Detection of Bacterial Spot Disease Resistance Genes Bs2 and Bs3 in Pepper
作者:
高升华1; 李宁1; 王飞1; JUNTAWONG Niran2; 焦春海3; 姚明华1
（1.湖北省农业科学院 经济作物研究所/蔬菜种质创新与遗传改良湖北省重点实验室，湖北 武汉 430064；2.泰国农业大学，泰国 曼谷 10900;3.湖北省农业科学院，湖北 武汉 430064）
Author(s):
Establishment and Application of Duplex PCR to Detection of Bacterial Spot Disease Resistance Genes Bs2 and Bs3 in Pepper
(1.Cash Crops Research Institute，Hubei Academy of Agricultural Sciences，Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement，Wuhan，Hubei 430064;2.Kasetsart University，Bangkok，Thailand 10900;3.Hubei Academy of Agricultural Sciences，Wuhan，Hubei 430064)
关键词:
辣椒; 抗辣椒疮痂病基因Bs2和Bs3; 双重PCR标记
Keywords:
pepper; Bs2 and Bs3; duplex PCR marker
DOI:
10.11937/bfyy.20194298
文献标志码:
A
摘要:
以栽培辣椒和野生型辣椒为试验材料，采用双重PCR体系构建的方法，建立了辣椒抗疮痂病基因Bs2和Bs3的双重PCR反应体系，以期利用该反应体系提高辣椒抗疮痂病种质资源鉴定和育种分离世代材料分子标记辅助筛选的效率。结果表明：抗疮痂病基因Bs2和Bs3的双重PCR反应体系，以Bs2-For/Bs2-Rev和Bs3-For/Bs3-Rev为特异引物，在退火温度为52 ℃时，具有很好的多态性和稳定性。此外，用该体系对不同辣椒种材料进行抗病性鉴定，与接种鉴定的结果一致，进一步验证了该体系的可靠性。该双重PCR反应体系可用于辣椒抗疮痂病品种选育。
Abstract:
Taking cultivated and wild-type peppers as materials,the construction method of duplex PCR system was used to establish a novel duplex PCR marker for detection of bacterial spot disease (BS) resistance genes Bs2 and Bs3,in order to improve the efficiency of germplasm resource identification and molecular marker-assisted screening of breeding generations.The results showed that the duplex PCR marker for detection of bacterial spot disease (BS) resistance genes Bs2 and Bs3 had rich polymorphism and high stability,when Bs2-For/Bs2-Rev and Bs3-For/Bs3-Rev were used as specific primers pairs and annealing temperature was set at 52 ℃.In addition,the novel duplex PCR system was used to identify the resistance of different pepper species materials to bacterial spot disease,and the results were consistent with the inoculation assay,indicating that the duplex PCR system had high reliability.The application of the duplex PCR system in Bs2 and Bs3 gene could improve breeding selection efficiency.

参考文献/References:

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备注/Memo

第一作者简介：高升华（1986-），男，博士，助理研究员，现主要从事辣椒遗传育种等研究工作。E-mail：gaoshenghua1986@126.com.责任作者：姚明华（1969-），男，博士，研究员，现主要从事辣椒遗传育种等研究工作。E-mail：yaomh_2008@126.com.基金项目：国家重点研发计划资助项目(2017YFD0101903，2016YFE0205500)；博士后科学基金资助项目(2017M620305)；湖北省技术创新专项资助项目(2017ABA147)；现代农业产业技术体系建设专项资金资助项目(CARS-23-G28)。收稿日期：2020-03-10

更新日期/Last Update: 2020-09-02