[1]牛强,刘悦,刘畅,等.响应面法优化软枣猕猴桃枝条多糖提取工艺[J].北方园艺,2020,44(09):118-124.[doi:10.11937/bfyy.20192688]
　NIU Qiang,LIU Yue,LIU Chang,et al.Optimization of Extraction Technology of Polysaccharides From the Branches of Actinidia arguta by Response Surface Methodology[J].Northern Horticulture,2020,44(09):118-124.[doi:10.11937/bfyy.20192688]

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响应面法优化软枣猕猴桃枝条多糖提取工艺

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷期数: 2020年09 页码: 118-124 栏目: 出版日期: 2020-05-15

Title:: Optimization of Extraction Technology of Polysaccharides From the Branches of Actinidia arguta by Response Surface Methodology

作者:: 牛强1; 2; 刘悦2; 刘畅2; 孙宏莱2; N.V.斯克里普琴科2; 3; 刘德江2; （1.佳木斯大学药学院，黑龙江佳木斯 154007；2.中-乌农林技术开发与应用国际合作联合实验室，黑龙江佳木斯 154007；3.乌克兰国家科学院M.M.格里什科国家植物园，乌克兰基辅 01014）

Author(s):: NIU Qiang1; 2; LIU Yue2; LIU Chang2; SUN Honglai2; N.V.Skrypchenko2; 3; LIU Dejiang2; (1.College of Pharmacy,Jiamusi University,Jiamusi,Heilongjiang 154007；2.China-Ukraine Agriculture & Forestry Technology Development and Application International Cooperation Joint Lab,Jiamusi,Heilongjiang 154007；3.M.M.Gryshko National Botanical Gardens of National Academy of Sciences of Ukraine,Kyiv,Ukraine 01014)

关键词:: 软枣猕猴桃枝条; 多糖; 响应面优化; 提取

Keywords:: Actinidia arguta branch; polysaccharides; response surface method optimization; extraction

DOI:: 10.11937/bfyy.20192688

文献标志码:: A

摘要:: 以软枣猕猴桃枝条为试材，通过响应面法优化软枣猕猴桃枝条多糖（polysaccharides from the branches of Actinidia arguta）的提取工艺，采用苯酚-硫酸法对软枣猕猴桃枝条多糖定量分析，在单因素试验的基础上，利用响应面法对提取软枣猕猴桃枝条多糖的各个因素进行优化。结果表明：响应面法优化多糖提取的最佳工艺为液料比29∶1 mL·g-1，提取温度80 ℃，提取时间128 min，在最优条件下多糖的平均提取率为3.31%。该研究采用响应面法对软枣猕猴桃枝条多糖的提取条件优化合理，为今后软枣猕猴桃的开发利用提供参考依据。

Abstract:: Using the branches of Actinidia arguta as the raw material to optimize the extraction technology of polysaccharides from the branches of Actinidia arguta (Sieb.et Zucc.) Planch.ex Miq.by response surface methodology(RSM).Phenol-sulfuric acid method was used to quantitatively analyze.On the basis of single factor experiment,response surface methodology was applied to optimize the main extraction conditions.The best conditions for the response surface optimization were as follows,liquid-material ratio 29∶1 mL·g-1,extraction temperature 80 ℃,and extracting time 128 minutes.The extraction rate of polysaccharide was 3.31% under the optimal conditions.The extraction conditions of polysaccharides from the branches of Actinidia arguta (Sieb.et Zucc.)Planch.ex Miq.were optimized by response surface method.This result could provide a theoretical basis for the further exploitation and utilization of Actinidia arguta.

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备注/Memo

第一作者简介：牛强（1995-），男，硕士研究生，研究方向为植物药研究与开发。E-mail:niuqiang7777@163.com.责任作者：刘德江（1980-），男，博士，高级实验师，现主要从事寒地植物资源、生态、栽培及其提取物等研究工作。E-mail:Liudejiang2004@163.com.基金项目：佳木斯大学校长创新创业基金资助项目(XZYF2019-18)；科技部高端外国专家引进计划资助项目（G20190211004）；中央财政支持地方高校发展基金资助项目；北药与功能食品特色学科建设资助项目。收稿日期：2019-09-27

更新日期/Last Update: 2020-07-24