WANG Fang,HAN Haozhang,WANG Xiaoli,et al.Effect of Saline-alkali Stress on the Number of Microorganisms in Rhizosphere Soil of Cinnamomum bodinieri Seedlings[J].Northern Horticulture,2020,44(09):111-117.[doi:10.11937/bfyy.20193237]
盐碱胁迫对猴樟幼苗根际土壤微生物数量的影响
- Title:
- Effect of Saline-alkali Stress on the Number of Microorganisms in Rhizosphere Soil of Cinnamomum bodinieri Seedlings
- Keywords:
- saline-alkali stress; soil microorganism; Cinnamomum bodinieri; soil physical and chemical properties
- 文献标志码:
- A
- 摘要:
- 以2年生盆栽猴樟幼苗为试材,采用不同浓度的Na2CO3和NaHCO3（物质的量比Na2CO3∶NaHCO3=1∶1）的混合溶液进行浇灌处理，采用定期测定土壤中真菌、细菌和放线菌的数量以及土壤可溶性盐含量、pH和电导率的方法，旨在探讨盐碱胁迫对猴樟幼苗根际土壤微生物数量的影响。结果表明：盐碱胁迫提高猴樟幼苗根际土壤pH、电导率和水溶性盐总量，浓度越高越明显。随着胁迫天数的增加，pH变化不明显，电导率先降低再明显升高，可溶性盐总量明显升高。盐碱胁迫初期，100、200 mmol·L-1浓度处理的根际土壤细菌数量显著高于对照，而300 mmol·L-1浓度处理的显著低于对照。盐碱胁迫促使放线菌数量显著低于对照、真菌数量显著高于对照，浓度越高越明显；随着胁迫天数的增加，对照和盐碱处理的猴樟幼苗根际土壤中细菌、真菌和放线菌的数量呈升高趋势，第25天后趋于稳定。盐碱胁迫降低土壤放线菌数量和细菌数量，提高了土壤真菌数量，低浓度盐碱胁迫初期提高细菌数量，真菌和细菌可能与猴樟的耐盐碱性有关。
- Abstract:
- The two-year-old potted seedlings of Cinnamomum bodinieri were taken as the material,were watered with mixed solution of Na2CO3 and NaHCO3 with different concentration（material ratio:Na2CO3∶NaHCO3=1∶1）,and tested the quantity of fungi,bacteria and actinomycetes in soil and the content of soil soluble salt,pH and electrical conductivity.Effects of saline-alkali stress on the number of microorganisms in rhizosphere soil of Cinnamomum bodinieri seedlings were studied.The results showed that pH,electrical conductivity and the content of soluble salt of rhizosphere soil of Cinnamomum bodinieri seedlings could be improved by saline-alkali stress,which was more obvious with higher concentration.But with the increase of stress time,changing in pH was not obvious,the electrical conductivity decreased first and then increased,and the content of soluble salt increased obviously.At the beginning of saline-alkali stress,the number of bacteria in rhizosphere soil at 100 mmol·L-1 and 200 mmol·L-1 concentrations were higher than that of the control,while the number of bacteria in rhizosphere soil at 300 mmol·L-1 concentrations was lower than that of the control.The number of actinomycetes was lower than that of control significantly forced by saline-alkali stress and the number of fungi was higher than that of control significantly,it was more obvious if the concentration was higher.The number of bacteria,fungi and actinomycetes in rhizosphere soil of Cinnamomum bodinieri seedlings of control and those treated by saline-alkali stress increased with the increase of stress time,and after the 25 days,it tended to be stable.Saline-alkali stress could reduce the number of soil actinomycetes and bacteria and increase the number of soil fungi.At the beginning of low concentration saline-alkali stress,the number of bacteria would be increased.Fungi and bacteria might be related to the salt and alkali resistance of Cinnamomum bodinieri.
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备注/Memo
第一作者简介：王芳（1982-），女，江苏宿迁人，硕士，讲师，现主要从事土壤生态学等研究工作。E-mail:835729199@qq.com.责任作者：韩浩章（1978-），男，江苏宿迁人，博士，副教授，现主要从事樟属植物种质资源创新与应用等研究工作。E-mail:123117186@qq.com.基金项目：江苏省苏北科技发展计划-科技富民强县资助项目（SZ-SQ2018047）；宿迁市重点研发计划资助项目（L201702）。收稿日期：2019-11-12