ZHAO Zheng,SHI Hongmei,GAI Shupeng,et al.Effects of Post-ripening on the Content and Components of Fatty Acid in Tree Peony Seed[J].Northern Horticulture,2019,43(04):106-111.[doi:10.11937/bfyy.20182607]
牡丹籽仁发育及后熟对其脂肪酸含量和成分的影响
- Title:
- Effects of Post-ripening on the Content and Components of Fatty Acid in Tree Peony Seed
- Keywords:
- tree peony; seed development; fatty acid; post-ripening; GC-MS
- 文献标志码:
- A
- 摘要:
- 以油用牡丹品种‘凤丹’为试材,记录了蓇葖果和籽仁不同发育时期的形态及质量,并利用GC-MS测定脂肪酸含量,分析了后熟对脂肪酸含量和成分的影响,以期为牡丹籽粒收获和脂肪酸生产提供指导。结果表明:籽仁发育过程可分为籽仁发育初期、籽仁快速生长期、籽仁发育定型期和籽仁成熟期4个时期。未经后熟处理的情况下,授粉后120 d时含油量最高,之后下降。后熟处理显著提高了籽仁的不饱和脂肪酸(油酸、亚油酸和α-亚麻酸)及总脂肪酸含量,其中100 d,后熟30 d的脂肪酸和不饱和脂肪酸含量最高,因此100 d采收后熟30 d是较佳的采收组合方式。研究结果对油用牡丹高产栽培具有重要指导意义,也为研究后熟机制奠定了基础。
- Abstract:
- The oil-used tree peony cultivar ‘Feng Dan’ was used as experiment material,and the effect of post-ripening on the content and components of fatty acids was analyzed by observing the morphology and weight of follicle and seed at differently developmental stage in order to provide guide for peony industry.The development processes were composed of the early formation stage,rapid growth stage,the developmental setting stage and mature stage,respectively.Under the non-post-ripening condition,the highest content of fatty acid was found in 120 days after pollination,and then followed by decline.Post-ripening treatments significantly increased the contents of unsaturated fatty acid and the total fatty acid,among which the contents of unsaturated fatty acid and the total fatty acid were the highest in 100 days after pollination and 30 days post-ripening.Therefore,the combination of harvesting after pollination 100 days adding post-ripening 30 days was a better way.The research results had important guiding significance for high-yield cultivation of oil-used tree peony and provided the theoretical basis for studying the mechanism of post-ripening in tree peony.
参考文献/References:
[1]WANG C Z,XU L,WU Q,et al.The importance of ultrahigh pressure processing over the quality of the extracted oil from peony seeds(Paeonia suffruticosa Andr.)[J].Industrial Crops and Products,2015,76:1142-1147.[2]NING C,JIANG Y,MENG J,et al.Herbaceous peony seed oil:A rich source of unsaturated fatty acids and γ-tocopherol[J].European Journal of Lipid Science and Technology,2015,117:532-542.[3]LI S S,YUAN R Y,CHEN L G,et al.Systematic qualitative and quantitative assessment of fatty acids in the seeds of 60 tree peony(Paeonia section Moutan DC.) cultivars by GC-MS[J].Food Chemistry,2015,173:133-140.[4]FAN J L,ZHU W X,KANG H B,et al.Flavonoid constituents and antioxidant capacity in flowers of different Zhongyuan tree peony cultivars[J].Journal of Functional Foods,2012,4(1):147-157.[5]刘炤,韩继刚,李晓青,等.‘凤丹’种子成熟过程中脂肪酸的累积规律[J].经济林研究,2015,33(4):75-80.[6]韩继刚,李晓青,刘炤,等.牡丹油用价值及其应用前景[J].粮食与油脂,2014,27(5):21-25.[7]李子璇,秦公伟,何建华,等.紫斑牡丹种仁种皮中脂肪酸组成比较分析[J].种子,2010,29(1):34-36.[8]HE C N,PENG Y,XIAO W,et al.Determination of chemical variability of phenolic and monoterpene glycosides in the seeds of Paeonia species using HPLC and profiling analysis[J].Food Chemistry,2013,138(4):2108-2114.[9]NEHDI I A.Cupressus sempervirens var.horizentalis seed oil:Chemical composition,physicochemical characteristics,and utilizations[J].Industrial Crops and Products,2013,41:381-385.[10]魏冬峰,张利霞,常青山,等.氮磷钾平衡施肥对凤丹光合特性的影响[J].核农学报,2016,30(11):2265-2273.[11]任磊,王雁,周琳,等.牡丹〖STBX〗PsMADS5〖STBZ〗基因的克隆、表达及载体构建[J].核农学报,2011,25(5):939-944.[12]黄兴琳,陆俊杏,廖冰楠,等.油用牡丹脂肪酸脱氢酶基因〖STBX〗FAD3〖STBZ〗的克隆与表达分析[J].中国农业科学,2017,50(10):1914-1921.[13]史国安,郭香凤,张改娜,等.油用牡丹籽粒长期贮藏劣变对其油脂品质的影响[J].食品工业科技,2017,38(13):277-280.[14]LI S S,WANG L S,SHU Q Y,et al.Fatty acid composition of developing tree peony(Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development[J].BMC Genomics,2015(16):208.[15]PARVINI F,ZEINANLOO A A,EBRAHIMIE E,et al.Differential expression of fatty acid desaturases in Mari and Shengeh olive cultivars during fruit development and ripening[J].European Journal of Lipid Science and Technology,2015,117(4):523-531.[16]EVARISTO A B,GROSSI J A S,PIMENTEL L D,et al.Harvest and post-harvest conditions influencing macauba(Acrocomia aculeata) oil quality attributes[J].Industrial Crops and Products,2016,85:63-73.[17]TIBALDI G,EMANUELA F,NI S.Cultivation practices do not change the Salvia sclarea L.essential oil but drying process does[J].Journal of Food Agriculture and Environment,2010(8):790-794.[18]LIU C,DING F,HAO F,et al.Reprogramming of seed metabolism facilitates preharvest sprouting resistance of wheat[J].Scientific Reports,2016(6):20593.[19]MORSCHER F,KRANNER I,ARC E,et al.Glutathione redox state,tocochromanols,fatty acids,antioxidant enzymes and protein carbonylation in sunflower seed embryos associated with after-ripening and ageing[J].Annals of Botany,2015,116:669-678.[20]刘雪娇,鹿保鑫,马楠.大豆后熟期对主要脂肪酸的影响[J].黑龙江八一农垦大学学报,2018,30(2):43-46.[21]景新明,郑光华,裴颜龙.野生紫斑牡丹和四川牡丹种子萌发特性及其致濒的关系[J].生物多样性,1995,3(2):84-87.[22]崔虎亮,黄弄璋,刘欣,等.不同贮藏条件对油用牡丹种子出油率的影响[J].中国种业,2016(10):28-30.[23]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.动植物油脂,脂肪酸甲脂的气相色谱分析:GB/T17377-2008[S].北京:中国标准出版社,2008.[24]马雪情,刘春洋,黄少峻,等.牡丹籽粒发育特性与营养成分动态变化的研究[J].中国粮油学报,2016,31(5):71-75.[25]AZIM G,BERND H.Seed yield,oil content and fatty acid composition of Oenothera biennis L.affected by harvest date and harvest method[J].Industrial Crops and Products,2007,25(3):274-281.[26]叶珊,王为宇,周敏樱,等.不同采收成熟度和堆沤方式对香榧种子堆沤后熟品质的影响[J].林业科学,2017,53(11):43-51.[27]王衍彬,刘本同,秦玉川,等.不同品种香榧种子油脂肪酸及香味物质组成分析[J].中国油脂,2016,41(2):101-105.[28]FENG J L,YANG Z J,BAI W W,et al.Transcriptome comparative analysis of two Camellia species reveals lipid metabolism during mature seed natural drying[J].Trees,2017(31):1827-1848.[29]DUSˇKOVA′E,DUSˇEK K,INDRA′K P,et al.Postharvest changes in essential oil content and quality of lavender flowers[J].Industrial Crops and Products,2016,79:225-231.[30]TIBALDI G,FONTANA E,NICOLA S.Postharvest management affects spearmint and calamint essential oils[J].Journal of Agricultural and Food Chemistry,2013,93:580-586.[31]曹永庆,姚小华,任华东,等.油茶果实矿质元素含量和油脂积累的相关性[J].中南林业科技大学学报,2013,33(10):38-41.[32]牛一川,安建平,陈双恩,等.亚麻籽粒中主要脂肪酸积累过程研究[J].西北农业学报,2004,13(3):26-30.[33]张思河,尹田夫,王萍,等.3个熟期类型大豆品种发育籽粒脂肪酸组分变化[J].西北农业学报,2000,9(1):83-86.
相似文献/References:
[1]郭丽丽,李 军,李金航,等.不同时期盆栽牡丹叶片RNA的提取研究[J].北方园艺,2014,38(12):99.
GUO Li-li,LI Jun,LI Jin-hang,et al.Extraction of Total RNA From Potted Tree Peony Leaves at Different Stages[J].Northern Horticulture,2014,38(04):99.
[2]刘 娜,秦安臣,陈 雪,等.牡丹对B9花期调控的响应研究[J].北方园艺,2014,38(10):56.
LIU Na,QIN An-chen,CHEN Xue,et al.Study on the Peony’s Flowering Regulation Response to B9[J].Northern Horticulture,2014,38(04):56.
[3]杨德翠,郑国生.柱枝孢叶斑病侵染对牡丹生理特性的影响[J].北方园艺,2014,38(01):57.
YANG De-cui,ZHENG Guo-sheng.Effects of Infection by Cylindrocladium canadenseon Physiological Characteristics of Tree Peony[J].Northern Horticulture,2014,38(04):57.
[4]高宏秀,张莹.牡丹春节催花技术[J].北方园艺,2014,38(06):72.
GAO Hong-xiu,ZHANG Ying.Flower Forcing Techniques of Tree Peony in Winter[J].Northern Horticulture,2014,38(04):72.
[5]郑玲,程彦伟.六个洛阳牡丹品种解剖结构与抗旱性关系研究[J].北方园艺,2013,37(01):65.
ZHENG Ling,CHENG Yan-wei.Study on Relationship Between Anatomical Structures and Drought Resistance of Some Cultivars of Luoyang Paeonia suffruticosa Andr.[J].Northern Horticulture,2013,37(04):65.
[6]史倩倩,王雁,周琳,等.生根粉及不同基质对牡丹传统品种扦插的影响[J].北方园艺,2012,36(01):91.
SHI Qian-qian,WANG Yan,ZHOU Lin,et al.Influence of Rooting Powder and Culture Substrata on Rooting of Different Traditional Cultivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(04):91.
[7]王 玮,张旭东,施 江,等.不同品种牡丹萌芽过程中组织抗冷性的变化[J].北方园艺,2013,37(06):52.
WANG Wei,ZHANG Xu-dong,SHI Jiang,et al.Changes of Cold Resistance of Floral Organs During Sprout in Different Tree Peony Cultivars[J].Northern Horticulture,2013,37(04):52.
[8]刘帅,丰震,徐艳,等.牡丹不同品种花期差异的生理机理研究[J].北方园艺,2012,36(03):67.
LIU Shuai,FENG Zhen,XU Yan,et al.Study on the Physiological Mechanism of Flowering Phase Difference in Cutivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(04):67.
[9]关丽霞.牡丹沙培嫩茎段离体培养的初步研究[J].北方园艺,2012,36(04):74.
GUAN Li-xia.Preliminary Study on in Vitro Culture of Sand-cultured Tender Stem Segments of Paeonia suffruticosa[J].Northern Horticulture,2012,36(04):74.
[10]尤扬,贾文庆,王慧.牡丹顶芽组织培养的初步研究[J].北方园艺,2012,36(04):131.
YOU Yang,JIA Wen-qing,WANG Hui.Preliminary Study on the Tissue Culture of Terminal Bud of Peony[J].Northern Horticulture,2012,36(04):131.
备注/Memo
第一作者简介:赵正(1994-),男,硕士研究生,研究方向为牡丹分子生物学。E-mail:634449307@qq.com.责任作者:张玉喜(1977-),女,教授,硕士生导师,现主要从事牡丹分子生物学等研究工作。E-mail:Zhang-yuxi@163.com.基金项目:国家自然科学基金面上资助项目(31471908,31372104)。收稿日期:2018-10-08