LIUJing,SHIMing,QIAOGaixia.RestorationofVitrifiedTest-tubePlantletsofLyciumbarbarum[J].Northern Horticulture,2019,43(03):138-143.[doi:10.11937/bfyy.20181421]
枸杞玻璃化组培苗恢复技术
- Title:
- RestorationofVitrifiedTest-tubePlantletsofLyciumbarbarum
- 文章编号:
- 20181421
- 文献标志码:
- A
- 摘要:
- 摘要：以枸杞不同程度玻璃化组培苗为试材，采用组织培养法，研究了不同基本培养基、外源添加物、添加聚丙烯酰胺、改变培养条件及玻璃化苗脱水处理在玻璃化组培苗恢复中的作用，探索出最佳的枸杞玻璃化组培苗恢复方法。结果表明：1/2MS+6-BA05mg?L-1+活性炭05g?L-1为枸杞轻度玻璃化苗恢复最佳培养基，适宜枸杞中度玻璃化苗恢复培养基为1/4MS+6-BA05mg?L-1+活性炭10g?L-1。当添加聚丙烯酰胺8、16g?L-1，枸杞轻度、中度玻璃化苗恢复率均达到最高，聚丙烯酰胺对枸杞玻璃化的组培苗有明显的逆转效果，逆转后的组培苗生长良好。经过脱水处理后，玻璃化苗明显得到恢复。脱水2d轻度玻璃化苗的恢复率可达7761%。随着脱水的天数增加，枸杞中度玻璃化苗恢复率逐渐提高，当脱水天数达3d时，恢复率最高达7124%。培养期间增强封口膜的透气性和光照强度能有效提高枸杞玻璃化苗恢复率。
- Abstract:
- Abstract:Theeffectsofdifferentbasicmedium,exogenousadditives,addingpolyacrylamide,differentcultureconditionsandshootsofdehydrationonvitrificationshootsrecoverybydifferentvitrifiedseedlingsofLyciumbarbarumasexplantsfortissueculturewerestudied.Themainpurposeofthestudywasexplorethemeasuresofwhichmakethevitrificationshootsrecovery.Theresultsshowedthat1/2MS+6-BA05mg?L-1+activatedcarbon05g?L-1wasthebestmediumforthemildvitreousseedlingsrecoveryofLyciumbarbarum.ItwassuitableforthemoderatevitrifiedseedlingsrecoveryofLyciumbarbarum1/4MS+6-BA05mg?L-1+activatedcarbon10g?L-1.Whenpolyacrylamide8g?L-1and16g?L-1wereadded,therecoveryrateofmildvitreousandmoderatevitrifiedseedlingsofLyciumbarbarumwasthehighest.ItnotonlycouldsignificantlyinhibitthegenerationofvitrficationofLyciumbarbarum,butalsohadsignificantreversaleffectwithvitrifiedtissue-culturingplantlets,whichgrewwellafterreverse.Afterdehydrationtreatment,thevitrifiedseedlingswereobviouslyrecovered.Therecoveryrateoflightvitrifiedseedlingsofdehydrated2dayscouldreach7761%.Withtheincreaseofthenumberofdaysofdehydration,therecoveryrateofmediumvitrifiedseedlingsofLyciumbarbarumwasgraduallyincreased.Whenthenumberofdehydrationdaysreached3days,thehighestrecoveryratecouldreach7124%.Enhancingmembranepermeabilityandlightintensitycouldalsoraisetherecoveryrateofvitrifiedseedlings.
参考文献/References:
[1]胡彦,赵艳.植物组织培养技术的应用以及在培养过程中存在的问题[J].陕西师范大学学报(自然科学版),2004(32):130-134.[2]罗青,张曦燕,李晓莺,等.不同培养条件对枸杞组培苗玻璃化的影响[J].安徽农业科学,2008(22):9400-9401.[3]蔡祖国,徐小彪,周会萍.植物组织培养中的玻璃化现象及其预防[J].植物技术通讯,2005,16(3):35-31.[4]戴丽娜,于志鹏,吕国华,等.薰衣草玻璃化组培苗逆转技术研究[J].新疆农业科学,2012,49(11):2054-2061.[5]齐红岩,贾卓男,陈岩.网纹甜瓜试管苗玻璃化成因研究[J].河南农业科学,2009(3):86-88.[6]王丽华.银胶菊组培技术及玻璃化防治措施研究[D].雅安:四川农业大学,2005.[7]王宏伟.豆梨组织培养过程中玻璃化形成机制及其恢复技术研究[D].南京:南京农业大学,2011.
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备注/Memo
第一作者简介：刘静（1989-），女，硕士，助理研究员，现主要从事生物技术等研究工作。E-mail:1137416502@qq.com.基金项目：宁夏自然科学基金资助项目（NZ16216）。收稿日期：2018-07-12