HUANGXiaohui,WANGChunhui,XUNing,et al.ToleranceandAccumulationofFourHeavyMetalsinMyceliumofFlammulinavelutipesandPleurotuseryngii[J].Northern Horticulture,2019,43(03):143-150.[doi:10.11937/bfyy.20182862]
金针菇、杏鲍菇菌丝对四种重金属耐受和富集特性的研究
- Title:
- ToleranceandAccumulationofFourHeavyMetalsinMyceliumofFlammulinavelutipesandPleurotuseryngii
- 文章编号:
- 20182862
- Keywords:
- Keywords:heavymatal; Lentinulaedodes; Flammulinavelutipes; mycelia; tolerance; accumlation
- 文献标志码:
- A
- 摘要:
- 摘要：以金针菇和杏鲍菇菌为试材，在平板培养基和液体发酵培养基中添加不同浓度梯度Pb、Cd、As、Hg重金属，通过测量菌丝日均生长速度、菌丝体干质量、菌丝体及发酵液中重金属含量等指标，进行重金属富集模型建模，研究不同菌类对不同种类重金属耐受及富集特征，以期为菌类富集重金属机理提供参考依据。结果表明：重金属Pb、Cd、As、Hg对菌丝生长及菌丝球量表现为低浓度（低于1mg?L-1）促进、高浓度（高于5mg?L-1）抑制，适宜浓度（1～5mg?L-1浓度）的重金属Pb、Cd、As对菌丝的生长有一个明显前期抑制后期菌丝恢复正常生长的现象。金针菇和杏鲍菇菌丝体均表现为对重金属Cd和Pb高度富集，最大富集量分别达到Cd619、424mg?kg-1DW以及Pb449、844mg?kg-1DW，对重金属As和Hg富集量较小，最大富集量分别为As49、21mg?kg-1DW以及Hg44、40mg?kg-1DW。不同菌类菌丝体对同一种重金属的富集饱和点不同，如金针菇菌丝体对Pb的富集饱和点是452mg?kg-1DW，杏鲍菇菌丝体对Pb的富集饱和点是902mg?kg-1DW。同一菌类对不同重金属的富集饱和点差异较大，如杏鲍菇对Cd的富集饱和点为429mg?kg-1DW，对As的富集饱和点为224mg?kg-1DW。
- Abstract:
- Abstract:TheexperimentofheavymetaltoleranceandaccumulationinFlammulinavelutipesandPleurotuseryngiimyceliumwascarriedoutthroughexternallyaddingdifferentconcentrationgradientsofPb,Cd,As,Hgtotheplatemediumandliquidfermentationmedium.Thetoleranceandaccumulationcharacteristicsofdifferentkindsofheavymetalsindifferentfungiwerestudiedbymeasuringtheaveragegrowthrateofmycelium,thedryweightofmycelium,aswellastheheavymetalconcentrationinmyceliumandfermentationbroth.Basedonthis,theheavymetalaccumulationmodelwasestablished,onthepurposeofprovidingfundamentaldatafortheheavymetalaccumulationmechanisminfungi.TheresultsshowedthatPb,Cd,As,Hg,atlowerconcentration(below1mg?L-1),promotedthegrowthofmyceliumandincreasedtheamountofmyceliumball,whiletheheavymetals,athigherconcentration(above5mg?L-1),hadaninhibitoryeffect.AndPb,CdandAs,atthemediumconcentration(1-5mg?L-1),hadanobviousinhibitoryeffectattheformerstage,butthemyceliumrecoveredtonormalgrowingspeedatthelatterstage.ThemyceliumofFlammulinavelutipesandPleurotuseryngiiaccumulatedhighamountofCdandPbwithCdreaching619mg?kg-1DW,424mg?kg-1DWandPbreaching449mg?kg-1DW,844mg?kg-1DW,butlowamountofAsandHgwasaccumulated,withAsonlyreaching49mg?kg-1DW,21mg?kg-1DWandHgreaching44mg?kg-1DW,40mg?kg-1DW.Differentmyceliumshaddifferentaccumulationsaturabilityforthesameheavymetal.TheaccumulationsaturationpointofPbinFlammulinavelutipesmyceliumwas452mg?kg-1DW,whilethatofPbinPleurotuseryngiimyceliumwasashighas902mg?kg-1DW.Meanwhile,thesamespeciesalsohaddifferentsaturabilityfordifferentfungi,andthedifferencewaslarge.TheaccumulationsaturationpointofCdinPleurotuseryngiimyceliumwas429mg?kg-1DW,butthatofAswas224mg?kg-1DW.
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备注/Memo
第一作者简介：黄晓辉（1984-），女，硕士，助理研究员，现主要从事食用菌副产业循环利用及农业微生态修复等研究工作。E-mail:38534098@qq.com.基金项目：国家科技计划课题资助项目（2013BAD16B04）；湖南省重点研发资助项目（2016NK2054）。收稿日期：2018-10-17