ZHANG Xibin,MA Dongmei.Genetic Diversity of SSR Markers in Salt Tolerant Alfalfa[J].Northern Horticulture,2018,42(20):40-47.[doi:10.11937/bfyy.20180572]
耐盐紫花苜蓿SSR标记遗传多样性分析
- Title:
- Genetic Diversity of SSR Markers in Salt Tolerant Alfalfa
- Keywords:
- alfalfa; genetic diversity; SSR; salt tolerant
- 文献标志码:
- A
- 摘要:
- 以5份不同耐盐紫花苜蓿为试材，利用5对SSR引物对其进行了遗传多样性检测，采用0/1法统计带型数据，并利用主成分分析和隶属函数法对5份紫花苜蓿的杂合度、香农指数、PIC、多态位点百分率、PNe/Ao综合评价。根据综合评价值（D值）对遗传变异大小进行排序，以期为后期遗传亲本的选择提供依据。结果表明：5个紫花苜蓿品种各位点各群体的平均DNA多态率为62.65%、有效等位基数平均数为3.66，低于等位基因观测值的平均数7.20、平均杂合度为0.736 6、平均PIC为0.689 0、平均香农指数为2.083；遗传变异程度大小为“巨能7号”（‘Juneng No.7’）>“抗旱15”（‘Kanghan 15’）>“巨能”（‘Juneng’）>“骑士T”（‘Qishi T’）>“阿迪娜”（‘Adina’）；遗传距离为0.434 2~0.865 6；聚类分析表明“骑士T”/“阿迪娜”之间的遗传距离最小，为0.314 4，“巨能7号”/“抗旱15”之间的遗传距离最大，为0.725 0。
- Abstract:
- Five different salt tolerant alfalfa varieties were used as materials for genetic diversity by 5 pairs of SSR primers,with statistical data by 0/1 method,and using principal component analysis and the method of membership function of 5 alfalfa heterozygosity,Shannon index,PIC,the percentage of polymorphic loci,PNe/Ao comprehensive evaluation.According to the comprehensive evaluation value (D value),the size of genetic variation was sorted,in order to provide the basis for the selection of later genetic parents.The results showed that the average DNA polymorphism of five alfalfa varieties to each group,effective rate was 62.65% and the average was 3.66,lower than the average number of the number of alleles observed value of 7.20,the average heterozygosity was 0.736 6,the average PIC was 0.689 0,the average Shannon-Wiener index was 2.083;the genetic variation was：‘Juneng No.7’> ‘Kanghan 15’>‘Juneng’>‘Qishi T’ >‘Adina’;genetic distance was 0.434 2-0.865 6;cluster analysis showed that the genetic distance between ‘Qishi T’/‘Adina’ was minimum(0.314 4),‘Juneng No.7’/‘Kanghan 15’ had the furthest genetic distance（0.725 0）.
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备注/Memo
第一作者简介：张喜斌(1989-)，男，硕士研究生，研究方向为植物基因工程。E-mail：1350302020@qq.com.责任作者：麻冬梅（1978-），女，博士，讲师，现主要从事植物抗逆生理等研究工作。E-mail：mdm319@tom.com.基金项目：宁夏回族自治区牧草育种专项资助项目（NYYZ-2014-Z12）。收稿日期：2018-04-17