ZHANG Yuan,ZHANG Wanrong,LIN Chun,et al.Establishment of Tissue Culture and Regeneration System of Purple Tomato With Rich Anthocyanidin[J].Northern Horticulture,2018,42(18):47-51.[doi:10.11937/bfyy.20180312]
富花青素紫色番茄组织培养再生体系的建立
- Title:
- Establishment of Tissue Culture and Regeneration System of Purple Tomato With Rich Anthocyanidin
- Keywords:
- purple tomato; callus; adventitious bud; rooting; regeneration system
- 文献标志码:
- A
- 摘要:
- 以樱桃型紫色番茄“砚紫1号”种子为试材，通过无菌处理获得无菌苗，用无菌苗的幼嫩子叶为外植体，研究了诱导愈伤组织分化及生根情况， 以期建立高效的离体培养再生体系。结果表明：紫色番茄无菌苗子叶在MS+IAA 0.1 mg·L-1+6-BA 0.5 mg·L-1培养基条件下的愈伤组织诱导状态最佳；将愈伤组织进行暗培养处理，可诱导愈伤产生不定芽，其中最佳的培养基条件是MS+NAA 0.01 mg·L-1+6-BA 2.0 mg·L-1；诱导再生的不定芽经过2周光培养接种至MS+NAA 0.05 mg·L-1培养基中，诱导生根良好，可获得完整的再生植株。
- Abstract:
- Cherry purple tomato ‘Yanzi No.1’ seeds were taken as experimental materials,and the sterile seedlings were obtained after sterile treatment;and then the immature cotyledons of sterile seedlings were used as explants for callus induction,differentiation and rooting were studied,and a complete tissue culture regeneration system was established.The results showed that purple tomato cotyledon cultured in MS+IAA 0.1 mg·L-1+6-BA 0.5 mg·L-1 was the best under the condition of callus induction medium;callus dark culture could induce callus to produce adventitious buds,the culture medium MS+NAA 0.01 mg·L-1+6-BA 2.0 mg·L-1 was the best;inducing regeneration of adventitious buds were cultured after 2 weeks of light in MS+NAA 0.05 mg·L-1 medium,rooting was fine,a complete regeneration system could be obtained.
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备注/Memo
第一作者简介：张园（1986-），女，博士研究生，研究方向为植物组织培养。E-mail：136151701@163.com.责任作者：刘正杰（1984-），男，博士，讲师，研究方向为蔬菜功能基因组学。E-mail：lzj1022@163.com.基金项目：云南省应用基础研究计划资助项目(2015FB143)。收稿日期：2018-03-12