WU Wenneng,ZHANG Qi,LEI Jiqing,et al.Identification and Pharmaceutical Screening of Kiwifruit Soft Rot Disease on ‘Guichang’ Gooseberry[J].Northern Horticulture,2018,42(16):47-54.[doi:10.11937/bfyy.20180087]
“贵长”猕猴桃软腐病病原菌分离鉴定及抑菌药剂筛选
- Title:
- Identification and Pharmaceutical Screening of Kiwifruit Soft Rot Disease on ‘Guichang’ Gooseberry
- 文献标志码:
- A
- 摘要:
- 为了明确“贵长”猕猴桃软腐病病原菌种类并筛选有效防治药剂，对贵州修文县部分果园“贵长”猕猴桃软腐病的致病菌株进行了分离、纯化、形态学及分子生物学鉴定，并对分离得到的病原菌开展致病性测定，同时采用菌丝生长速率法测定了20余种杀菌剂对猕猴桃主要致病菌的室内毒力。结果表明：“贵长”猕猴桃软腐病的致病菌株种类为葡萄座腔菌(Botryosphaeria dothidea)、拟茎点霉菌(Phomopsis sp.)、茎点霉菌(Phona sp.)和链格孢菌(Alternaria alternata)，其中葡萄座腔菌和拟茎点霉菌为软腐病的主要致病菌。葡萄座腔菌和拟茎点霉菌室内毒力表明，20余种杀菌剂中以肟菌酯戊唑醇、己唑醇、戊唑醇、异菌脲、苯醚甲环唑对猕猴桃软腐病主要致病菌葡萄座腔菌(Botryosphaeria dothidea)和拟茎点霉菌(Phomopsis sp.)均表现出较强的抑菌活性，其EC50分别为0.038 0~0.156 8 μg·mL-1和0.017 4~0.643 6 μg·mL-1，推荐作为猕猴桃软腐病的有效防控药剂。
- Abstract:
- To identify the pathogenic fungus of kiwifruit soft rot disease to ‘Guichang’ Gooseberry in Xiuwen county,and screen out effective chemicals for control of this disease.The pathogenic fungus of kiwifruit soft rot disease to ‘Guichang’ Gooseberry were isolated,purified and identified by using the morphological and molecular biological observation.Then,the toxicities of more than fungicides were determined by mycelial growth rate.The results showed that four kinds of fungi were responsible for fruit rot of kiwifruit,which were Botryosphaeria dothidea,Phomopsis sp.,Alternaria alternata and Phona sp..Among them,Botryosphaeria dothidea and Phomopsis sp.were the dominant pathogen.Trifloxystrobin tebuconazole,Tebuconazole,Hexaconazole,Iiprodione and Difenoconazole showed good inhibitive effect against Botryosphaeria dothidea and Phomopsis sp..The EC50 values of this five fungicides to Botryosphaeria dothidea were 0.038 0-0.1 56 8 μg·mL-1,respectively,and those to Phomopsis sp.were 0.017 4-0.643 6 μg·mL-1,which could be used as an effective control agent for soft rot of kiwifruit in the field.
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备注/Memo
第一作者简介：吴文能（1983-），男，博士，副教授，现主要从事农作物病虫害综合防治等研究工作。E-mail:wuwenneng123@126.com.责任作者：王瑞（1979-），男，博士，教授，现主要从事农产品采后贮藏与保鲜等研究工作。E-mail:wangrui060729@126.com.基金项目：贵州省自然科技基金资助项目（黔科合基础[2016]1006)）；贵州省普通高等学校科技拔尖人才支持计划资助项目（黔教合KY字[2016]091）；贵州省省级果蔬发展资金资助项目（黔农函[2017]12号）；贵阳市科技计划资助项目（筑科合同[20161001]农1-7）；贵阳学院人才引进基金资助项目；贵州省百人计划资助项目（20164022）。收稿日期：2018-02-01