HE Hua,WANG Yan,PAN Hui,et al.Annual Investigation of Kiwifruit Disease in Pingbian Area of Yunnan Province and Construction of Control Model[J].Northern Horticulture,2021,(11):26-36.[doi:10.11937/bfyy.20203998]
云南屏边猕猴桃病害周年调查及防治模型构建
- Title:
- Annual Investigation of Kiwifruit Disease in Pingbian Area of Yunnan Province and Construction of Control Model
- Keywords:
- kiwifruit; disease; canker; soft rot; control model construction
- 文献标志码:
- A
- 摘要:
- 以云南屏边县7个代表性猕猴桃园区周年调查所获得的病害样本为试材，通过采集大量病害样本，结合分离纯化、生物学特征、分子生物学鉴定及反接验证结果对病原菌进行分析，研究了7个园区四季发生的病害种类和分布，并提出针对性的防治措施，以此构建了屏边猕猴桃园区的病害防治模型，以期为屏边县猕猴桃病害的综合防治提供参考依据，促进猕猴桃产业的发展。结果表明：屏边县猕猴桃主要病害为细菌性溃疡病、真菌性黑斑病、褐斑病、叶斑病、炭疽病、果实软腐病;1、3、5号园区的春季和4号园区的春夏出现细菌性溃疡病；3号园区发生间座壳菌和拟盘多毛孢属2种软腐病；秋冬季软腐病为最主要病害，1、2、3、4号园区均有发生；7号园区有黑孢菌属引起的叶斑病发生。可参考该防治模型进行综合防治才能显著改善该地区猕猴桃病害。
- Abstract:
- The disease samples gained by the annual investigation in the seven typical kiwifruit orchards in Pingbian county of Yunnan were used as test materials，and the research was performed by collecting a large number of diseases，combining with the separation and purification，biological characteristics and molecular identification and reverse validation，analyzing the pathogens，studying the disease types and distribution，and putting forward corresponding control measures，which contribute to construct the Pingbian disease control model of kiwifruit orchards，provide a reference basis for the integrated control of diseases，and promote the development of kiwifruit industry.The results showed that，the main kiwifruit diseases in Pingbian include bacterial canker disease，fungal black spot disease，brown spot disease，leaf spot disease，anthrax，and fruit soft rot；bacterial canker disease was the main disease in the spring of No.1，No.3，and No.5，and the spring and summer of No.4 orchard；the 2 kinds of soft rot Diaporthe sp.and Pestalotiopsis sp.happened in No.3；soft rot was the main disease in autumn and winter，and happening in No.1，No.2，No.3 and No.4；the leaf spot diseasec aused by Nigrospora sp.occurred in No.7.Integrated control based on the control model could be used to significantly improve the disease.
参考文献/References:
[1]孙雷明,方金豹.我国猕猴桃种质资源的保存与研究利用[J].植物遗传资源学报，2020,21(6):1483-1493.[2]KAI K，BI W，SUI Y,et al.Curcumin inhibits Diaporthe phaseolorum and reduces postharvest decay in kiwifruit[J].Scientia Horticulturae,2020,259:108860.[3]PAN L，ZHAO X，CHEN M,et al.Effect of exogenous methyl jasmonate treatment on disease resistance of postharvest kiwifruit[J].Food Chemistry,2020,305:125483.[4]潘慧,邓蕾,陈美艳,等.浙江省泰顺县猕猴桃周年病害调查及病原菌鉴定[J].江苏农业科学,2019,47(14):104-111.[5]SAWADA H，FUJIKAWA T.Genetic diversity of Pseudomonas syringae pv.actinidiae，pathogen of kiwifruit bacterial canker[J].Plant Pathology,2019,68(7):1235-1248.[6]李黎,陈美艳,张鹏,等.猕猴桃软腐病的病原菌鉴定[J].植物保护学报,2016,43(3):527-528.[7]潘慧,陈美艳,张胜菊,等.云南省屏边县猕猴桃春季主要病害调查及病原鉴定[C].昆明:中国园艺学会2017年年会,2017.[8]段程久.屏边县猕猴桃产业发展现状分析[J].云南农业,2019(11):46-48.[9]REES-GEORGE J，VANNESTE J L，CORNISH D A,et al．Detection of Pseudomonas syringe pv.Actinidiae using polymerase chain reaction (PCR) primers based on the 16S-23S rDNA inter-transcribed spacer region and comparison with PCR primers based on other gene regions[J].Plant Pathology，2010，59(3):453-464.[10]余卓桐,罗大全,谢艺贤,等.橡胶树主要病害防治决策模型研究[J].中国热带农业,2006(3):26-29.[11]潘慧,胡秋舲,张胜菊,等.贵州六盘水市猕猴桃病害调查及病原鉴定[J].植物保护,2018,44(4):125-131.[12]张文娟.猕猴桃溃疡病拮抗菌株筛选及田间药效试验[J].陕西农业科学,2020,66(5):36-38.[13]YE Z F，WEI P F，XUE S Z,et al.Characterization of Pseudomonas syringae pv.actinidiae Biovar 3 strain and cultivar tolerance to kiwifruit bacterial canker in China[J].Journal of Plant Pathology，2019，102(2):443-450.[14]KINUGAWA M，SATO T.Kiwifruit dieback caused by Diaporthe sp.and Botryosphaeria dothidea，kiwifruit soft rot fungi[J].Annals of the Phytopathological Society of Japan,2003,69(4):373-383.[15]DIAZ G A，LATORRE B A，JARA S,et al.First report of Diaporthe novem causing postharvest rot of kiwifruit during controlled atmosphere storage in chile[J].Plant Disease，2014,98(9):1274.[16]ZHANG D，BI W，KAI K,et al.Effect of chlorogenic acid on controlling kiwifruit postharvest decay caused by Diaporthe sp.[J].LWT,2020,132:109805.[17]范先敏.猕猴桃软腐病拮抗菌筛选及初步拮抗机理研究[D].武汉:华中农业大学,2017．[18]PAN H，CHEN M Y，DENG L,et al.First report of Didymella glomeratacausing black spot disease of kiwifruit in China[J].Plant Disease,2018,102(12):2654.[19]MORAL J，LICHTEMBERG P S F，PAPAGELIS A,et al.Didymella glomerate causing leaf blight on pistachio[J].European Journal of Plant Pathology,2018,151(4):1095-1099.[20]张献辉,李红敏,李全胜.解淀粉芽孢杆菌ZJ01及其复配制剂对枣黑斑病的防治效果[J].江苏农业科学,2019,47(13):137-139.[21]王坦,陶爱丽,黄思良,等.2种药剂混用对金叶女贞黑斑病的防治效果[J].农药，2012,51:64-65.[22]李爱华,郭小成.秦美猕猴桃软腐病的发生规律与防治初探[J].陕西农业科学,1994(3):44.[23]王小洁,李士谣,李亚巍,等.猕猴桃软腐病病原菌的分离鉴定及其防治药剂筛选[J].植物保护学报,2017,44(5):826-832.[24]管斌,吕兴萍,徐超,等.红叶石楠小孢拟盘多毛孢叶斑病化学防治试验[J].西北林学院学报,2013,28(2):131-135.[25]张凯东,秦双林,周英,等.生防菌对猕猴桃两种病原真菌的抑制效果[J].中国南方果树,2020,49(1):123-125.[26]赵金梅,高贵田,谷留杰,等.中华猕猴桃褐斑病病原鉴定及抑菌药剂筛选[J].中国农业科学,2013,46(23):4916-4925.[27]KIM P I，RYU J，KIM Y H,et al.Production of biosurfactant lipopeptides Iturin A，fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides[J].Journal of Microbiology and Biotechnology,2010,20(1):138-145.[28]ASHWINI N，SRIVIDYA S.Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1.3[J].Biotech,2014,4(2)：127-136.[29]胡容平,石军,陈晓娟,等.四川省猕猴桃贮藏期炭疽病生物学特性及防治研究[J].安徽农业科学,2019,47(13):132-135.[30]CHEN X Y L，ZHANG C，YANG H,et al.First report of leaf spot on Chenopodium album caused by Nigrospora pyriformis in China[J].Plant Disease，2020,104(6):1872.[31]吴琪.大连市草坪病害调查及草坪草黑孢霉叶斑病的初步研究[D].长春:吉林农业大学,2008.[32]林多多.棉花叶斑病病原真菌鉴定及室内药剂筛选[D].南京:南京师范大学,2009.[33]马叶,张春冬.几种杀菌剂防治香蕉叶斑病田间药效试验[J].中国南方果树,2011,40(5):55-56.[34]GHASEMI S，AHMADIAN G，JELODAR N B,et al.Antifungal chitinases from Bacillus pumilus SG2：Preliminary report[J].World Journal of Microbiology and Biotechnology,2010,26(8):1437-1443.[35]AHMAD Z，WU J，CHEN L,et al.Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR[J].Scientific Reports,2017,7(1):1-13.
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备注/Memo
第一作者简介：何华（1981-），女，硕士，讲师，现主要从事园林植物病虫害等研究工作。E-mail：395919116@qq.com.责任作者：陈凯（1986-），男，博士，讲师，现主要从事采后生物技术等研究工作。E-mail：kadychen2015@163.com.基金项目：国家自然科学基金面上资助项目（31972156）；湖北工业大学博士科研启动基金资助项目（BSQD2019035）。收稿日期：2020-09-16