[1]陈为,张琳.油用牡丹FAD2基因的生物信息学分析[J].北方园艺,2018,42(07):77-81.[doi:10.11937/bfyy.20173113]
　CHEN Wei,ZHANG Lin.Bioinformatic Analysis of [STHX]FAD2 Gene in Paeonia ostii[J].Northern Horticulture,2018,42(07):77-81.[doi:10.11937/bfyy.20173113]
点击复制

油用牡丹FAD2基因的生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年07 页码: 77-81 栏目: 出版日期: 2018-04-10
Title:
Bioinformatic Analysis of [STHX]FAD2 Gene in Paeonia ostii
作者:
陈为; 张琳
（洛阳理工学院 环境工程与化学学院，河南 洛阳 471000）
Author(s):
CHEN Wei; ZHANG Lin
(School of Environmental Engineering and Chemistry,Luoyang Institute of Science and Technology,Luoyang,Henan 471000)
关键词:
FAD2; 牡丹; 生物信息学
Keywords:
FAD2; Paeonia ostii; bioinformatics
DOI:
10.11937/bfyy.20173113
文献标志码:
A
摘要:
以油用牡丹“凤丹”（Paeonia ostii）为试材，采用生物信息学方法，对调控多不饱和脂肪酸合成的关键酶ω-6脂肪酸脱氢酶（Fatty acid desaturase,FAD2）基因进行生物信息学分析，以期为进一步研究PoFAD2基因调控牡丹多不饱和脂肪酸合成提供参考。结果表明：油用牡丹3个FAD2蛋白被聚类到了3个分支，预示着这3个基因存在功能上的差异。其中PoFAD2-1 cDNA全长1 695 bp，编码383个氨基酸。PoFAD2-1无信号肽结构，是非分泌型蛋白，有3个FAD2特有的组氨酸保守区和4个跨膜结构域，亚细胞定位预测该基因定位于内质网，与目前所报道的其它物种的FAD2-1一致。
Abstract:
In order to further study the PoFAD2 gene in regulating polyunsaturated fatty acid synthesis,the FAD2 gene coding ω-6 fatty acid dehydrogenase in peony ‘Fengdan’(Paeonia ostii) was analyzed via bioinformatics methods.The results showed that the three FAD2 proteins of Paeonia ostii were clustered into 3 branches,indicating the varied functions among them.The full-length cDNA of PoFAD2-1 was 1 695 bp,encoding 383 amino acids.It was a secreted protein without signal peptide structure.PoFAD2-1 had four transmembrane regions,including three conserved histidine clusters,located in the endoplasmic reticulum,which were in line with the FAD2 proteins in other species.

参考文献/References:

 

[1]LI S S,YUAN R Y,CHEN L G,et al.Systematic qualitative and quantitative assessment of fatty acids in the seeds of 60 tree peony (Paeonia section Moutan DC.) cultivars by GC-MS[J].Food Chemistry,2015,173:133-140.

[2]OKULEY J,LIGHTNER J,FELDMANN K,et al. Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis[J].Plant Cell,1994,6(1):147-158.

[3]SCHLUETER J A,VASYLENKO-SANDERS I F,DESHPANDE S,et al.The FAD2 gene family of soybean:Insights into the structural and functional divergence of a paleopolyploid genome[J].Crop Science,2007,47(1):14-26.

[4]HOSHINO T,TAKAGI Y,ANAI T.Novel GmFAD2-1b mutant alleles created by reverse genetics induce marked elevation of oleic acid content in soybean seeds in combination with GmFAD2-1a mutant alleles[J].Breeding Science,2010,60(4):419-425.

[5]PHAM A T,LEE J D,SHANNON J G,et al.Mutant alleles of FAD2-1A and FAD2-1B combine to produce soybeans with the high oleic acid seed oil trait[J].BMC Plant Biology,2010,10(1):195.

[6]PHAM A T,LEE J D,SHANNON J G,et al.A novel FAD2-1A allele in a soybean plant introduction offers an alternate means to produce soybean seed oil with 85% oleic acid content[J].Theoretical & Applied Genetics,2011,123(5):793-802.

[7]ZHANG D,PIRTLE I L,PARK S J,et al.Identification and expression of a new delta-12 fatty acid desaturase (FAD2-4) gene in upland cotton and its functional expression in yeast and Arabidopsis thaliana plants[J].Plant Physiology & Biochemistry,2009,47(6):462-471.

[8]LOO F J V D,BROUN P,TURNER S,et al.An oleate 12-hydroxylase from Ricinus communis L.is a fatty acyl desaturase homolog[J].Proceedings of the National Academy of Sciences of the United States of America,1995,92(15):6743-6747.

[9]BROUN P,SOMERVILLE C.Accumulation of ricinoleic,lesquerolic,and densipolic acids in seeds of transgenic Arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean[J].Plant Physiology,1997,113(3):933-942.

[10]LEE M,LENMAN M,BANAS〖KG-2mm〗〖JX-+1mm〗＇ 〖JX+1mm〗A,et al.Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation[J].Science,1998,280(5365):915-918.

[11]VOINNET O,RIVAS S,MESTRE P,et al.An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus[J].Plant Journal for Cell & Molecular Biology,2003,33(5):949-956.

[12]CAHOON E B,KINNEY A J.Dimorphecolic acid is synthesized by the coordinate activities of two divergent Delta12-oleic acid desaturases[J].Journal of Biological Chemistry,2004,279(13):495-502.

[13]BROUN P,BODDUPALLI S,SOMERVILLE C.A bifunctional oleate 12-hydroxylase:Desaturase from Lesquerella fendleri[J].Plant Journal,1998,13(2):201-210.

[14]CARLSSON A S,THOMAEUS S,HAMBERG M,et al.Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes[J].European Journal of Biochemistry,2004,271(14):2991-2997.

[15]LI S S,WANG L S,SHU Q Y,et al.Fatty acid composition of developing tree peony(Paeonia,section Moutan,DC.)seeds and transcriptome analysis during seed development[J].BMC Genomics,2015,16(1):208.

[16]MOCHE M,SHANKLIN J,GHOSHAL A,et al.Azide and acetate complexes plus two iron-depleted crystal structures of the di-iron enzyme delta9 stearoyl-acyl carrier protein desaturase:Implications for oxygen activation and catalytic intermediates[J].Journal of Biological Chemistry,2003,278(27):25072-25080.

[17]SHANKLIN J,WHITTLE E,FOX B G.Eight histidine residues are catalytically essential in a membrane-associated iron enzyme,stearoyl-CoA desaturase,and are conserved in alkane hydroxylase and xylene monooxygenase[J].Biochemistry,1994,33(43):12787-12794.

相似文献/References:

[1]郭丽丽,李 军,李金航,等.不同时期盆栽牡丹叶片RNA的提取研究[J].北方园艺,2014,38(12):99.
　GUO Li-li,LI Jun,LI Jin-hang,et al.Extraction of Total RNA From Potted Tree Peony Leaves at Different Stages[J].Northern Horticulture,2014,38(07):99.
[2]刘 娜,秦安臣,陈 雪,等.牡丹对B9花期调控的响应研究[J].北方园艺,2014,38(10):56.
　LIU Na,QIN An-chen,CHEN Xue,et al.Study on the Peony’s Flowering Regulation Response to B9[J].Northern Horticulture,2014,38(07):56.
[3]杨德翠,郑国生.柱枝孢叶斑病侵染对牡丹生理特性的影响[J].北方园艺,2014,38(01):57.
　YANG De-cui,ZHENG Guo-sheng.Effects of Infection by Cylindrocladium canadenseon Physiological Characteristics of Tree Peony[J].Northern Horticulture,2014,38(07):57.
[4]高宏秀,张莹.牡丹春节催花技术[J].北方园艺,2014,38(06):72.
　GAO Hong-xiu,ZHANG Ying.Flower Forcing Techniques of Tree Peony in Winter[J].Northern Horticulture,2014,38(07):72.
[5]郑玲,程彦伟.六个洛阳牡丹品种解剖结构与抗旱性关系研究[J].北方园艺,2013,37(01):65.
　ZHENG Ling,CHENG Yan-wei.Study on Relationship Between Anatomical Structures and Drought Resistance of Some Cultivars of Luoyang Paeonia suffruticosa Andr.[J].Northern Horticulture,2013,37(07):65.
[6]史倩倩,王雁,周琳,等.生根粉及不同基质对牡丹传统品种扦插的影响[J].北方园艺,2012,36(01):91.
　SHI Qian-qian,WANG Yan,ZHOU Lin,et al.Influence of Rooting Powder and Culture Substrata on Rooting of Different Traditional Cultivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(07):91.
[7]王 玮,张旭东,施 江,等.不同品种牡丹萌芽过程中组织抗冷性的变化[J].北方园艺,2013,37(06):52.
　WANG Wei,ZHANG Xu-dong,SHI Jiang,et al.Changes of Cold Resistance of Floral Organs During Sprout in Different Tree Peony Cultivars[J].Northern Horticulture,2013,37(07):52.
[8]刘帅,丰震,徐艳,等.牡丹不同品种花期差异的生理机理研究[J].北方园艺,2012,36(03):67.
　LIU Shuai,FENG Zhen,XU Yan,et al.Study on the Physiological Mechanism of Flowering Phase Difference in Cutivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(07):67.
[9]关丽霞.牡丹沙培嫩茎段离体培养的初步研究[J].北方园艺,2012,36(04):74.
　GUAN Li-xia.Preliminary Study on in Vitro Culture of Sand-cultured Tender Stem Segments of Paeonia suffruticosa[J].Northern Horticulture,2012,36(07):74.
[10]尤扬,贾文庆,王慧.牡丹顶芽组织培养的初步研究[J].北方园艺,2012,36(04):131.
　YOU Yang,JIA Wen-qing,WANG Hui.Preliminary Study on the Tissue Culture of Terminal Bud of Peony[J].Northern Horticulture,2012,36(07):131.

备注/Memo

第一作者简介：陈为（1981-），男，博士，讲师，现主要从事牡丹育种及油脂合成分子机理等研究工作。E-mail：parddy@hotmail.com.责任作者：张琳（1985-），女，博士，讲师，现主要从事牡丹育种及油脂合成分子机理等研究工作。E-mail：zlhelen@163.com.基金项目：河南省科技厅2017年科技攻关（农业）资助项目（172102110103）；河南省教育厅2016年高等学校重点科研资助项目（16A180030）；洛阳理工学院高层次人才科研资助项目（2014BZ02,2014BZ03）。收稿日期：2017-10-30

更新日期/Last Update: 2018-04-20