[1]王珊珊,李俊萍,刘孟,等.茎枯病菌毒素对芦笋愈伤组织防御酶活性的影响[J].北方园艺,2018,42(01):52-56.[doi:10.11937/bfyy.20171722]
　WANG Shanshan,LI Junping,LIU Meng,et al.Effects of Stem Blight Toxin on Defensive Enzymes inCallus of Asparagus officinalis L.[J].Northern Horticulture,2018,42(01):52-56.[doi:10.11937/bfyy.20171722]
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茎枯病菌毒素对芦笋愈伤组织防御酶活性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年01 页码: 52-56 栏目: 出版日期: 2018-01-10
Title:
Effects of Stem Blight Toxin on Defensive Enzymes inCallus of Asparagus officinalis L.
作者:
王珊珊; 李俊萍; 刘孟; 乜兰春
河北农业大学 园艺学院，河北省蔬菜种质创新与利用重点实验室，河北省蔬菜产业协同创新中心，河北 保定 071000
Author(s):
WANG Shanshan; LI Junping; LIU Meng; NIE Lanchun
College of Horticulture，Agricultural University of Hebei/Key Laboratory of Vegetable Germplasm and Utilization of Hebei/Collaborative Innovation Center of Vegetable Industry in Hebei，Baoding,Hebei 071000
关键词:
芦笋; 愈伤组织; 茎枯病菌毒素; 防御酶; 再生苗
Keywords:
asparagus; callus; stem blight pathogen toxin; defense enzyme; regeneration seedling
DOI:
10.11937/bfyy.20171722
文献标志码:
A
摘要:
以‘阿波罗’‘冠军’和‘NJ987’3个芦笋品种的愈伤组织为试材，研究了茎枯病菌毒素对芦笋愈伤组织防御酶活性的影响及再生苗抗性的诱导效应。结果表明：3个芦笋品种愈伤组织在茎枯病菌毒素胁迫下，超氧化物歧化酶（SOD)、过氧化物酶（POD)、过氧化氢酶（CAT)、苯丙氨酸解氨酶（PAL)和多酚氧化酶（PPO)活性均较对照显著升高。其中SOD、POD、PPO活性均呈先迅速升高，稍有下降后再次升高的趋势，并分别在第2、8天先后出现2个峰值。CAT活性在第2天达到峰值，之后呈降低趋势。‘冠军’愈伤组织PAL在接种后2 d内迅速升高达到峰值，之后一直保持较高水平；‘NJ987’ 和‘阿波罗’愈伤组织PAL则分别在第2、4天后才开始迅速上升，在第8天时达到峰值。经毒素处理后存活的愈伤组织再生苗对茎枯病菌的抗性增强。
Abstract:
Callus of 3 asparagus cultivars were taken as materials,effects of stem blight toxin on defensive enzymes in the callus of Asparagus officinalis L. and its function in the induced resistance of regeneration seedlings were studied.The results showed that the activities of SOD，POD，CAT，PAL，PPO in callus showed a trend of sharply increased after toxin treatment.The activity of SOD，POD，PPO in the callus of toxin treatment showed a trend of decline after rising first，and were always higher than the corresponding control.It had two peak values which presented at 2 days and 8 days.The activity of CAT also had a peak at 2 days，then it showed a trend of decline.The activity of PAL in the callus of ‘Champion’ reached the peak value quickly at 2 days after inoculation and kept a high level.The callus of ‘NJ987’ and ‘Apollo’ began to increase quickly at 2 days and 4 days and got the peak value at 8 days.Besides，toxin induced ‘Apollo’‘Champion’ and ‘NJ987’ callus resistance enhanced.

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备注/Memo

第一作者简介：王珊珊（1991-），女，硕士研究生，研究方向为蔬菜逆境生理与分子生物学。E-mail:15028256911@126.com.

责任作者：乜兰春（1966-），女，博士，教授，现主要从事蔬菜生理生态及生长调控等研究工作。
基金项目：河北省现代农业产业技术体系蔬菜创新团队资助项目（HBCT2013050201）。
收稿日期：2017-07-14

更新日期/Last Update: 2018-01-12