MAO Yun,CHEN Ganggang,WANG Xianlei,et al.Location of Powdery Mildew Resistance Gene in Xinjiang Melon ‘PHHL’[J].Northern Horticulture,2017,41(11):135-139.[doi:10.11937/bfyy.201711027]
新疆甜瓜品种‘PHHL’白粉病抗病基因初步定位
- Title:
- Location of Powdery Mildew Resistance Gene in Xinjiang Melon ‘PHHL’
- Keywords:
- melon; powdery mildew resistant gene; SSR marker; gene mapping
- 文献标志码:
- A
- 摘要:
- 以新疆甜瓜白粉病抗病品种‘PHHL’与感病品种‘XM2’为亲本，构建F2代遗传分离群体，研究了抗病品种‘PHHL’中白粉病抗性基因的遗传规律。采用BSA(Bulked Segregation Analysis)法和SSR（Simple Sequence Repeat）分子标记技术，对白粉病抗性基因进行遗传定位,为抗白粉病育种提供种质资源。结果表明：甜瓜‘PHHL’品系对白粉病Podosphaera xanthii的抗性受一个显性基因控制；筛选11个SSR多态性标记，通过连锁分析，将该基因定位于LGVII标记SSR12510与ECM123之间；327、328号标记发生了偏分离现象且差异显著，这2个标记存在偏分离位点。
- Abstract:
- In this experiment,the seqregation of resistance was evaluated in F2 population derived from a cross between the resistance melon ‘PHHL’ and the susceptible melon ‘XM2’.The major resistance gene was located by methods of Bulked Segregation Analysis (BSA) and Simple Sequence Repeat (SSR) markers，in order to provide the germplasm resources for resistance breeding to powdery mildew.The results showed that the resistant to powdery mildew Podosphaera xanthii was controlled by a dominant gene in melon ‘PHHL’ varieties；11 polymorphic markers were identified.The resistance gene was located on LGVII between SSR12510 and ECM123；the phenomenon of partial separation was appeared in No.327,328 marker and the difference was significant,these two markers existed partial separation site.
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备注/Memo
第一作者简介：毛云（1989-），男，硕士研究生，研究方向为植物学。E-mail：413842015@qq.com.责任作者：李冠（1949-），男，硕士，教授，博士生导师，研究方向为植物生理生化与分子生物学。E-mail：guanli@xju.edu.cn.基金项目：国家自然科学基金资助项目（31260258）。