YAN Han,YANG Ruixiu,YAO Yuan,et al.Diversity Analysis of Fusariumo oxysporum in Northeast of China[J].Northern Horticulture,2017,41(11):130-134.[doi:10.11937/bfyy.201711026]
东北地区甜瓜枯萎病菌遗传多样性分析
- Title:
- Diversity Analysis of Fusariumo oxysporum in Northeast of China
- Keywords:
- melon; Fusarium oxysporum; genetic diversity; SRAP; cluster analysis
- 文献标志码:
- A
- 摘要:
- 以44株来自东北不同地区甜瓜枯萎病株的尖孢镰孢菌为试材，采用SRAP分子标记技术，研究了甜瓜枯萎病菌的遗传多样性，以探究其遗传多样性与地理来源的关系。结果表明：从100对SRAP引物中筛选出多态性强、重复性好的8组SRAP引物组合，共扩增出53条条带，其中多态性条带53条，多态性比率为100%，平均每对引物扩增得到6.63条多态性条带。通过聚类分析，44株供试菌株相似系数为0.47～0.98，不同菌株之间的亲缘关系较近，遗传多样性较为丰富，但是仍存在遗传差异，且与地理来源没有明显的相关性。
- Abstract:
- Forty four isolates Fusarium oxysporum from melon in various areas of the Northeast were used as test materials,the genetic diversity were analyzed by SRAP,in order to explore the relationship between genetic diversity and geographical origin.The results indicated that 8 pairs primers which had rich product sand high polymorphism were selected，generated a total of 53 reproducible bands，53 of which were polymorphic,and similarity coefficient was 100%，with an average of 6.63 for each pair of primers.By cluster analysis,the similarity coefficients of 44 strains Fusarium oxysporum were ranged from 0.47 to 0.98,rich genetic variation existed among the tested isolates and there was no correlation between SRAP group and the geographic origin.
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备注/Memo
第一作者简介：延涵（1992-），女，内蒙古包头人，硕士研究生，研究方向为甜瓜枯萎病。E-mail:18609830557@163.com.责任作者：高增贵(1966-),男，内蒙古准格尔旗人，博士，教授，博士生导师，研究方向为玉米及蔬菜病害生物防治。E-mail：gaozenggui@sina.com.基金项目：公益性行业（农业）科研专项经费资助项目（201503110-04）；辽宁省联合基金资助项目（2015020759）。