WANG Fenghua,LI Guangyuan,LI Sheng,et al.Influence of Vernalization on Germination and Flowering of Cherry Radish[J].Northern Horticulture,2016,40(05):39-42.[doi:10.11937/bfyy.201605011]
低温春化对樱桃萝卜种子萌发及成花的影响
- Title:
- Influence of Vernalization on Germination and Flowering of Cherry Radish
- Keywords:
- radish; squaring; bolting; vernalization; endogenous hormone; flowering
- 文献标志码:
- A
- 摘要:
- 萝卜（Raphanus sativus L.）属十字花科根茎类蔬菜，在春季栽培中极易通过低温春化而抽薹开花。该研究以樱桃萝卜为试材，研究了低温春化对其种子萌发及抽薹开花的影响。结果表明：春化处理能够有效提高种子活力，促进种子萌发。低温春化时间越长，萝卜现蕾、抽薹及开花所需的时间就越短。成花过程中樱桃萝卜的可溶性蛋白质含量、叶绿素含量、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性在盛花期之前逐渐升高，之后逐渐下降。盛花期，春化处理20 d的可溶性蛋白质含量、POD活性、CAT〖KG-*4〗活性均显著高于处理5 d的。丙二醛（MDA）含量随着开花进程逐渐上升，春化时间的长短对叶绿素含量和MDA含量无显著影响。成花过程中可溶性糖含量变化不显著，但在现蕾期和抽薹期，春化处理20 d的可溶性糖含量显著高于处理5 d。成花过程中，玉米素（ZT）含量逐渐降低，20 d处理的ZT含量显著高于5 d处理。吲哚乙酸（IAA）含量随着抽薹开花逐渐升高，但5 d和20 d处理之间没有显著差异。该试验说明，樱桃萝卜抽薹开花与可溶性蛋白质含量、POD活性、CAT活性、MDA含量、ZT含量、IAA含量等的变化相关，春化时间长短在一定程度上影响上述指标。
- Abstract:
- Radish (Raphanus sativus L.) is one of root vegetables，which can easily go through vernalization，results in bolting and flowering then lost the edible value when cultivated in spring.The effect of vernalization on germination，bolting，flowering，and physiological index were studied in this research using cherry radish as material.The results showed that vernalization treatment improved the seed vigor，promoted seeds to germinate.The longer vernalization time，the shorter time was needed for squaring，bolting and flowering.The soluble protein content，Chl content，POD and CAT activities were all increased before full flowering，and then decreased.At the stage of full flowering，the soluble protein content，POD and CAT activities under 20-day-old treatment were all higher than those under 5-day-old treatment.While MDA content increased from squaring stage to flowering stage.No differences exited on Chl and MDA contents between 20-day-old and 5-day-old treatment.The content of soluble sugar kept in a stable level in the process of flowering.The content of soluble sugar under 20-day-old treatment was higher than that under 5-day-old treatment at the squaring and bolting stage.ZT content decreased in the process of flowing.ZT content under 20-day-old treatment was higher than that under 5-day-old treatment.On the contrary，IAA content increased from the stage of bolting to decline，but there were no differences between 5-day-old treatment and 20-day-old treatment.The test conclusions were bolting and flowering of cherry radish was related with the changes of soluble protein content，POD activity，CAT activity and MDA content，the content of ZT and IAA.Furthermore，the length of vernalization time affected the above index to a certain extent.
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备注/Memo
第一作者简介：王凤华（1972-），女，博士，副教授，研究方向为蔬菜生物学与生物技术。E-mail：fenghua123668@126.com.