GUO Xiaoyue,DING Yadi,DENG Jia,et al.Separation and Identification of Pathogenic Fungi in Blueberry Storage Period[J].Northern Horticulture,2015,39(24):104-108.[doi:10.11937/bfyy.201524030]
蓝莓贮藏期病原真菌的分离与鉴定
- Title:
- Separation and Identification of Pathogenic Fungi in Blueberry Storage Period
- Keywords:
- blueberry; pathogenic fungi; isolation; identification
- 文献标志码:
- A
- 摘要:
- 为明确云南省玉溪市蓝莓采后贮藏期引起果实腐烂的病原真菌种类，以采自澄江地区的蓝莓鲜果贮藏自然发病的果实为试验材料,采用组织分离法分离培养病原菌，经形态学观察和ITS rDNA（Internal transcribed spacer）区序列测定分析，对相关病原真菌进行了分类鉴定。结果表明:从采后贮藏过程中发生病害的蓝莓果实上分离到3株病原真菌，经鉴定3株真菌分别为枝孢菌属（Cladosporium）、链格孢属（Alternaria）和匍柄霉属（Stemphylium）；该研究可为澄江地区蓝莓贮藏期腐烂病害的预防和控制及进一步深入研究提供一定的参考理论依据。
- Abstract:
- In order to determine the pathogenic fungi species of storage rot blueberry in Yuxi City，Yunnan Province，the fruits were collected at Chengjiang County.Naturally infected fruits were used as material.Pathogenic bacteria was cultivated with tissue isolation.After the isolation and purification of fungi，pathogenic fungi were identified with morphological observation，ITS rDNA (Internal transcribed spacer) area sequence analysis.The results showed that three pathogenic fungi were separated from the disease occur on blueberry fruit during storage.Three fungi were Cladosporium，Alternaria and Stemphylium.The results of this study provided a theoretical foundation for blueberry storage rot disease prevention and control in Chengjiang County.
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备注/Memo
第一作者简介：郭晓月（1989-），女，硕士研究生，研究方向为果树学。E-mail:qiang_yuer @163.com.责任作者：邓佳（1983-），女，博士，讲师，现主要从事果树培育与利用等研究工作。E-mail:dengjia1983@163.com.基金项目：云南省教育厅科学研究基金资助项目（2014Y331）；国家自然科学基金资助项目（31460214）。